Rabbit Anti-Sheep IgG(H+L)-UNLB

Cat. No.
6150-01  
Size
1.0 mg  
Price (USD)
$50.00 
Specificity Reacts with the heavy and light chains of sheep IgG
Source Pooled antisera from rabbits hyperimmunized with sheep IgG
Cross Adsorption None; may react with immunoglobulins from other species and the light chains of other sheep immunoglobulins
Purification Affinity chromatography on sheep IgG covalently linked to agarose
Isotype Rabbit IgG
Isotype Control Rabbit IgG-UNLB
Format/Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Concentration 1.0 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Quality tested applications for relevant formats include -
ELISA 1-11
FLISA
Other referenced applications for relevant formats include -
Immunohistochemistry-Frozen Sections 12,13
Immunocytochemistry 14
Western Blot 8,15-18
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2796206
  • ELISA plate was coated with Rabbit Anti-Sheep IgG(H+L)-UNLB (SB Cat. No. 6150-01). Serially diluted Sheep IgG-BIOT (SB Cat. No. 0133-08) was captured followed by Streptavidin-HRP (SB Cat. No. 7100-05) and quantified.
  • FLISA plate was coated with Rabbit Anti-Sheep IgG(H+L)-UNLB (SB Cat. No. 6150-01). Serially diluted Sheep IgG-PE (SB Cat. No. 0133-09) was captured and fluorescence intensity quantified.

ELISA plate was coated with Rabbit Anti-Sheep IgG(H+L)-UNLB (SB Cat. No. 6150-01). Serially diluted Sheep IgG-BIOT (SB Cat. No. 0133-08) was captured followed by Streptavidin-HRP (SB Cat. No. 7100-05) and quantified.
 

FLISA plate was coated with Rabbit Anti-Sheep IgG(H+L)-UNLB (SB Cat. No. 6150-01). Serially diluted Sheep IgG-PE (SB Cat. No. 0133-09) was captured and fluorescence intensity quantified.
 
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References
1. Kennedy NJ, Spithill TW, Tennent J, Wood PR, Piedrafita D. DNA vaccines in sheep: CTLA-4 mediated targeting and CpG motifs enhance immunogenicity in a DNA prime/protein boost strategy. Vaccine. 2006;24:970-9. (ELISA)
2. Wee JL, Scheerlinck JY, Snibson KJ, Edwards S, Pearse M, Quinn C, et al. Pulmonary delivery of ISCOMATRIX influenza vaccine induces both systemic and mucosal immunity with antigen dose sparing. Mucosal Immunol. 2008;1:489-96. (ELISA)
3. Greenwood DL, Dynon K, Kalkanidis M, Xiang S, Plebanski M, Scheerlinck JY. Vaccination against foot-and-mouth disease virus using peptides conjugated to nano-beads. Vaccine. 2008;26:2706-13. (ELISA)
4. Carey AJ, Timms P, Rawlinson G, Brumm J, Nilsson K, Harris JM, et al. A multi-subunit chlamydial vaccine induces antibody and cell-mediated immunity in immunized koalas (Phascolarctos cinereus): comparison of three different adjuvants. Am J Reprod Immunol. 2010;63:161-72. (ELISA)
5. Vujanic A, Wee JL, Snibson KJ, Edwards S, Pearse M, Quinn C, et al. Combined mucosal and systemic immunity following pulmonary delivery of ISCOMATRIX™ adjuvanted recombinant antigens. Vaccine. 2010;28:2593-7. (ELISA)
6. Santarém VA, Chesine PA, Lamers BE, Rubinsky-Elefant G, Giuffrida R. Anti-Toxocara spp. antibodies in sheep from southeastern Brazil. Vet Parasitol. 2011;179:283-6. (ELISA)
7. Vujanic A, Snibson KJ, Wee JL, Edwards SJ, Pearse MJ, Scheerlinck JY, et al. Long-term antibody and immune memory response induced by pulmonary delivery of the influenza Iscomatrix vaccine. Clin Vaccine Immunol. 2012;19:79-83. (ELISA)
8. Mukherjee J, McCann C, Ofori K, Hill J, Baldwin K, Shoemaker CB, et al. Sheep monoclonal antibodies prevent systemic effects of botulinum neurotoxin A1. Toxins. 2012;4:1565-81. (ELISA, WB)
9. Tellez J, Van Vliet K, Tseng Y, Finn JD, Tschernia N, Almeida-Porada G, et al. Characterization of naturally-occurring humoral immunity to AAV in sheep. PLoS One. 2013;8(9):e75142. (ELISA)
10. Kollipara A, Polkinghorne A, Beagley KW, Timms P. Vaccination of koalas with a recombinant Chlamydia pecorum major outer membrane protein induces antibodies of different specificity compared to those following a natural live infection. PLoS One. 2013;8(9):e74808. (ELISA)
11. Gunawardana M, Baum MM, Smith TJ, Moss JA. An intravaginal ring for the sustained delivery of antibodies. J Pharm Sci. 2014;103:3611-20. (ELISA)
12. Raats CJ, van den Born J, Bakker MA, Oppers-Walgreen B, Pisa BJ, Dijkman HB, et al. Expression of agrin, dystroglycan, and utrophin in normal renal tissue and in experimental glomerulopathies. Am J Pathol. 2000;156:1749-65. (IHC-FS)
13. Ge Y, Jiang C, Sung SJ, Bagavant H, Dai C, Wang H, et al. Cgnz1 allele confers kidney resistance to damage preventing progression of immune complex-mediated acute lupus glomerulonephritis. J Exp Med. 2013;210:2387-401. (IHS-FS)
14. Peattie DA, Alonso RA, Hein A, Caulfield JP. Ultrastructural localization of giardins to the edges of disk microribbons of Giarida lamblia and the nucleotide and deduced protein sequence of alpha giardin. J Cell Biol. 1989;109:2323-35. (ICC)
15. Rosinha GM, Myioshi A, Azevedo V, Splitter GA, Oliveira SC. Molecular and immunological characterisation of recombinant Brucella abortus glyceraldehyde-3-phosphate-dehydrogenase, a T- and B-cell reactive protein that induces partial protection when co-administered with an interleukin-12-expressing plasmid in a DNA vaccine formulation. J Med Microbiol. 2002;51:661-71. (WB)
16. Kollipara A, George C, Hanger J, Loader J, Polkinghorne A, Beagley K, et al. Vaccination of healthy and diseased koalas (Phascolarctos cinereus) with a Chlamydia pecorum multi-subunit vaccine: evaluation of immunity and pathology. Vaccine. 2012;30:1875-85. (WB)
17. Hu D, Wang Y, Chen Z, Ma Z, You Q, Zhang X, et al. Artemisinin protects against dextran sulfate-sodium-induced inflammatory bowel disease, which is associated with activation of the pregnane X receptor. Eur J Pharmacol. 2014;738:273-84. (WB)
18. Hu D, Wang Y, Chen Z, Ma Z, You Q, Zhang X, et al. The protective effect of piperine on dextran sulfate sodium induced inflammatory bowel disease and its relation with pregnane X receptor activation. J Ethnopharmacol. 2015;169:109-23. (WB)