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  • Rabbit F(ab')2 Anti-Sheep IgG(H+L), Human SP ads-UNLB

Rabbit F(ab')2 Anti-Sheep IgG(H+L), Human SP ads-UNLB

Cat. No.:
6016-01
Purified Rabbit F(ab')2 Anti-Sheep IgG(H+L) antibody with minimal reactivity to human serum proteins for use in ELISA assays.
$99.00
Size Price (USD) Quantity
0.5 mg $99.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Rabbit F(ab')2 IgG
Isotype Control Rabbit F(ab')2 IgG-UNLB
Specificity Reacts with the heavy and light chains of sheep IgG
Source Pepsin digest of Rabbit Anti-Sheep IgG(H+L), Human SP ads (SB Cat. No. 6156)
Cross Adsorption Human serum proteins (SP); may react with immunoglobulins from other species and the light chains of other sheep immunoglobulins
Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Clonality Polyclonal
Concentration 0.5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C
Applications Quality tested applications for relevant formats include -
ELISA 1
FLISA

RRID Number AB_2796095

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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Other Conjugates and Formulations

References and Citations

  1. 1. Sturgill JL, Mathews J, Scherle P, Conrad DH. Glutamate signaling through the kainate receptor enhances human immunoglobulin production. J Neuroimmunol. 2011;233:80-9. (ELISA)

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  3. Human pancreatic carcinoma cell line MIA PaCa-2 was blocked with Normal Rabbit Serum (SB Cat. No. 0040-01) and stained with Mouse Anti-Human CD44-UNLB (SB Cat. No. 9400-01; right) followed by Rabbit Anti-Mouse IgG(H+L), Human ads-BIOT (SB Cat. No. 6175-08), Streptavidin-FITC (SB Cat. No. 7100-02), and DAPI.
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  4. ELISA plate was coated with purified sheep IgG. Immunoglobulin was detected with Rabbit F(ab')<sub>2</sub> Anti-Sheep IgG(H+L)-UNLB (SB Cat. No. 6010-01) followed by Goat Anti-Rabbit Ig, Human ads-UNLB (SB Cat. No. 4010-05).
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  5. ELISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Donkey F(ab')<sub>2</sub> Anti-Rabbit IgG(H+L), Multi-Species SP ads-HRP (SB Cat. No. 6444-05).
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  6. ELISA plate was coated with Rabbit Anti-Sheep IgG(H+L)-UNLB (SB Cat. No. 6150-01). Serially diluted Sheep IgG-BIOT (SB Cat. No. 0133-08) was captured followed by Streptavidin-HRP (SB Cat. No. 7100-05) and quantified.
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  7. ELISA plate was coated with purified sheep IgG and human serum. Immunoglobulin and serum were detected with Rabbit Anti-Sheep IgG(H+L), Human SP ads-UNLB (SB Cat. No. 6156-01) followed by Goat Anti-Rabbit IgG(H+L), Mouse/Human ads-HRP (SB Cat. No. 4050-05).
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  8. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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