Mouse Anti-Goat IgG Fc-UNLB

Cat. No.
6158-01  
Size
0.5 mg  
Price (USD)
$175.00 
Clone SB115d
Isotype Mouse (BALB/c) IgG2aκ
Isotype Control Mouse IgG2a-UNLB
Immunogen Pooled goat IgG
Specificity Goat IgG Fc
Minimal reactivity to goat IgG Fab and sheep, rabbit, human, monkey, rat, hamster, canine, and swine IgG
Format/Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
ELISA – Quality tested 1
FLISA – Quality tested
Western Blot – Reported in literature 2
Immunohistochemistry-Frozen Sections 3
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2796221
  • ELISA plate was coated with purified goat IgG Fc and IgG Fab. Immunoglobulins were detected with serially diluted Mouse Anti-Goat IgG Fc-UNLB (SB Cat. No. 6158-01) followed by Goat Anti-Mouse IgG2a, Human ads-HRP (SB Cat. No. 1080-05).

ELISA plate was coated with purified goat IgG Fc and IgG Fab. Immunoglobulins were detected with serially diluted Mouse Anti-Goat IgG Fc-UNLB (SB Cat. No. 6158-01) followed by Goat Anti-Mouse IgG2a, Human ads-HRP (SB Cat. No. 1080-05).
 
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References
1. Tang J, Sun X, Yang H, Zhang B, Li Z, Lin Z, et al. Well-oriented ZZ-PS-tag with high Fc-binding onto polystyrene surface for controlled immobilization of capture antibodies. Anal Chim Acta. 2013;776:74-8. (ELISA)
2. Mohapatra A, Van Dyken SJ, Schneider C, Nussbaum JC, Liang H, Locksley RM. Group 2 innate lymphoid cells utilize the IRF4-IL-9 module to coordinate epithelial cell maintenance of lung homeostasis. Mucosal Immunol. 2016;9:275-86. (WB)
3. SouthernBiotech published data (IHC-FS)