Mouse Anti-Human FADD-SEPH

Cat. No.
10941-25  
Size
50 tests  
Price (USD)
$360.00 
Clone 1A11
Isotype Mouse (BALB/c) IgG1κ
Immunogen Recombinant FADD
Specificity Human FADD
Alternalte Name(s) FAS-associated death domain protein, GIG3, MGC8528, MORT1
Description Fas-associated death domain (FADD, also known as MORT1) is an important apoptosis adapter molecule involved in the Fas-mediated cell death pathway. FADD associates with the intracellular domain of FAS (CD95/APO-1) through their respective C-terminal death domains (DD). The N-terminal region of FADD contains a death effectors domain (DED) which recruits and binds cytosolic proteins such as FLICE/caspase-8. The subsequent activation of FLICE by proteolytic cleavage leads to the activation of the caspase-mediated (ICE-related proteases) apoptotic cascade. Overexpression of FADD induces apoptosis which is inhibited by CrmA, a specific inhibitor of the interleukin-1β-converting enzyme. FADD has also been shown under certain circumstances to promote cell survival and proliferation.
Format/Conjugate SEPH (Agarose)
Buffer Formulation Borate buffered saline, pH 8.2
Volume 2.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
ELISA – Quality tested
Western Blot – Quality tested
Immunoprecipitation – Quality tested
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2794561
  • Total cell lysates from Jurkat cells were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Human FADD-UNLB (SB Cat. No. 10941-01). Proteins were visualized using Goat Anti-Mouse IgG, Human ads-HRP (SB Cat. No. 1030-05) secondary antibody and chemiluminescent detection.

Total cell lysates from Jurkat cells were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Human FADD-UNLB (SB Cat. No. 10941-01). Proteins were visualized using Goat Anti-Mouse IgG, Human ads-HRP (SB Cat. No. 1030-05) secondary antibody and chemiluminescent detection.
 
Related Products (8)
Sepharose® is a registered trademark of GE Healthcare