Rat Anti-Mouse IgD-BIOT

Cat. No.
1120-08  
Size
0.5 mg  
Price (USD)
$195.00 
Clone 11-26
Isotype Rat IgG2aκ
Isotype Control Rat IgG2a-BIOT
Specificity Mouse IgD
Format/Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 1-11,14
Immunohistochemistry-Frozen Sections – Reported in literature 10-17
Immunohistochemistry-Paraffin Sections – Reported in literature 23
Immunoprecipitation – Reported in literature 18,19
Separation – Reported in literature 5,20
Costimulation – Reported in literature 21
Proximity Ligation Assay – Reported in literature 22
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2631189
  • Frozen mouse spleen tissue was stained with Goat Anti-Mouse IgM, Human ads-TXRD (SB Cat. No. 1020-07) and Rat Anti-Mouse IgD-BIOT (SB Cat. No. 1120-08) followed by Streptavidin-AF488.

    Image from Ren W, Lagerstedt K, Grimsholm O, Stern A, Sun J, Fang Y, et al. Uncoupling of natural IgE production and CD23 surface expression levels. PLoS One. 2013;8(4):e62851. Figure 2(c)
  • BALB/c mouse splenocytes were stained with Rat Anti-Mouse IgD-BIOT (SB Cat. No. 1120-08) and Rat Anti-Mouse CD19-PE (SB Cat. No. 1575-09) followed by Streptavidin-FITC (SB Cat. No. 7100-02).

Frozen mouse spleen tissue was stained with Goat Anti-Mouse IgM, Human ads-TXRD (SB Cat. No. 1020-07) and Rat Anti-Mouse IgD-BIOT (SB Cat. No. 1120-08) followed by Streptavidin-AF488.

Image from Ren W, Lagerstedt K, Grimsholm O, Stern A, Sun J, Fang Y, et al. Uncoupling of natural IgE production and CD23 surface expression levels. PLoS One. 2013;8(4):e62851. Figure 2(c)
 

BALB/c mouse splenocytes were stained with Rat Anti-Mouse IgD-BIOT (SB Cat. No. 1120-08) and Rat Anti-Mouse CD19-PE (SB Cat. No. 1575-09) followed by Streptavidin-FITC (SB Cat. No. 7100-02).
 
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References
1. Rahman ZS, Rao SP, Kalled SL, Manser T. Normal induction but attenuated progression of germinal center responses in BAFF and BAFF-R signaling-deficient mice. J Exp Med. 2003;198:1157-69. (FC)
2. Gorelik L, Cutler AH, Thill G, Miklasz SD, Shea DE, Ambrose C, et al. Cutting edge: BAFF regulates CD21/35 and CD23 expression independent of its B cell survival function. J Immunol. 2004;172:762-6. (FC)
3. Cariappa A, Shoham T, Liu H, Levy S, Boucheix C, Pillai S. The CD9 tetraspanin is not required for the development of peripheral B cells or for humoral immunity. J Immunol. 2005;175:2925-30. (FC)
4. Erlandsson L, Licence S, Gaspal F, Lane P, Corcoran AE, Mårtensson I. Both the pre-BCR and the IL-7Rα are essential for expansion at the pre-BII cell stage in vivo. Eur J Immunol. 2005;35:1969-76. (FC)
5. Mattsson N, Duzevik EG, Pelsue SC. Expansion of CD22lo B cells in the spleen of autoimmune-prone flaky skin mice. Cell Immunol. 2005;234:124-32. (FC)
6. Klein U, Casola S, Cattoretti G, Shen Q, Lia M, Mo T, et al. Transcription factor IRF4 controls plasma cell differentiation and class-switch recombination. Nat Immunol. 2006;7:773-82. (FC, Sep)
7. Cariappa A, Boboila C, Moran ST, Liu H, Shi HN, Pillai S. The recirculating B cell pool contains two functionally distinct, long-lived, posttransitional, follicular B cell populations. J Immunol. 2007;179:2270-81. (FC)
8. Xiang Y, Garrard WT. The downstream transcriptional enhancer, Ed, positively regulates mouse Ig κ gene expression and somatic hypermutation. J Immunol. 2008;180:6725-32. (FC)
9. Mayne CG, Amanna IJ, Nashold FE, Hayes CE. Systemic autoimmunity in BAFF-R-mutant A/WySnJ strain mice. Eur J Immunol. 2008;38:587-98. (FC)
10. Coffey F, Manser T. Expression of cellular FLIP by B cells is required for their participation in an immune response. J Immunol. 2010;184:4871-9. (FC, IHC-FS)
11. Seeley-Fallen MK, Liu LJ, Shapiro MR, Onabajo OO, Palaniyandi S, Zhu X, et al. Actin-binding protein 1 links B-cell antigen receptors to negative signaling pathways. Proc Natl Acad Sci USA. 2014;111:9881-6. (FC, IHC-FS)
12. Pasparakis M, Alexopoulou L, Episkopou V, Kollias G. Immune and inflammatory responses in TNFα-deficient mice: a critical requirement for TNFα in the formation of primary B cell follicles, follicular dendritic cell networks and germinal centers, and in the maturation of the humoral immune response. J Exp Med. 1996;184:1397-411. (IHC-FS)
13. Wu X, Jiang N, Fang Y, Xu C, Mao D, Singh J, et al. Impaired affinity maturation in Cr2-/- mice is rescued by adjuvants without improvement in germinal center development. J Immunol. 2000;165:3119-27. (IHC-FS)
14. Rahman ZS, Niu H, Perry D, Wakeland E, Manser T, Morel L. Expression of the autoimmune Fcgr2b NZW allele fails to be upregulated in germinal center B cells and is associated with increased IgG production. Genes Immun. 2007;8:604-12. (FC, IHC-FS)
15. Gibb DR, Shikh ME, Kang D, Rowe WJ, El Sayed R, Cichy J, et al. ADAM10 is essential for Notch2-dependent marginal zone B cell development and CD23 cleavage in vivo. J Exp Med. 2010;207:623-35. (IHC-FS)
16. Simonetti G, Carette A, Silva K, Wang H, De Silva NS, Heise N, et al. IRF4 controls the positioning of mature B cells in the lymphoid microenvironments by regulating NOTCH2 expression and activity. J Exp Med. 2013;210:2887-902. (IHC-FS)
17. Soni C, Wong EB, Domeier PP, Khan TN, Satoh T, Akira S, et al. B cell-intrinsic TLR7 signaling is essential for the development of spontaneous germinal centers. J Immunol. 2014;193:4400-14. (IHC-FS)
18. Campbell KS, Cambier JC. B lymphocyte antigen receptors (mIg) are non-covalently associated with a disulfide linked, inducibly phosphorylated glycoprotein complex. EMBO J. 1990;9:441-8. (IP)
19. Hutchcroft JE, Harrison ML, Geahlen RL. Association of the 72-kDa protein-tyrosine kinase PTK72 with the B cell antigen receptor. J Biol Chem. 1992;267:8613-9. (IP)
20. Bowman EP, Kuklin NA, Youngman KR, Lazarus NH, Kunkel EJ, Pan J, et al. The intestinal chemokine thymus-expressed chemokine (CCL25) attracts IgA antibody-secreting cells. J Exp Med. 2002;195:269-75. (Sep)
21. El Shikh ME, El Sayed RM, Szakal AK, Tew JG. T-independent antibody responses to T-dependent antigens: a novel follicular dendritic cell-dependent activity. J Immunol. 2009;182:3482-91. (Costim)
22. Kläsener K, Maity PC, Hobeika E, Yang J, Reth M. B cell activation involves nanoscale receptor reorganizations and inside-out signaling by Syk. Elife. 2014;3:e02069. (Proximity ligation assay)
23. SouthernBiotech published data (IHC-PS)