Rat Anti-Mouse CD4-APC/CY5.5

Cat. No.
1540-18  
Size
0.1 mg  
Price (USD)
$205.00 
Clone GK1.5
Isotype Rat (Lewis) IgG2bκ
Isotype Control Rat IgG2b-APC/CY5.5
Immunogen Mouse CTL clone V4
Specificity Mouse CD4
Alternalte Name(s) L3T4, Ly-4
Description CD4 is a type I transmembrane glycoprotein expressed on most thymocytes and the helper/inducer subpopulation of mature T lymphocytes of all mouse strains tested. CD4 has also been detected at low density on pluripotent hematopoietic stem cells, bone marrow myeloid precursors, and intrathymic lymphoid precursors. The CD4 antigen functions as a co-receptor, interacting with class II major histocompatability complex (MHC) molecules in the recognition of foreign antigens by T cells. In vivo and in vitro treatment with the GK1.5 monoclonal antibody effectively depletes CD4+ cells. GK1.5 also blocks helper T cell responses to MHC class II antigens, including cytolysis, proliferation, allogeneic B cell help, and release of lymphokines.
Format/Conjugate APC/CY5.5 (Allophycocyanin/Cyanine 5.5)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide and a stabilizer
Concentration 0.1 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C; Avoid exposure to light; Do not freeze
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 1,5,7,11-15
Immunohistochemistry-Frozen Sections – Reported in literature 2-4
Immunocytochemistry – Reported in literature 5
Immunoprecipitation – Reported in literature 1
Depletion – Reported in literature 1,7,8
Separation – Reported in literature 6,9,10
Blocking – Reported in literature 1,8
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2794841
  • BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD4-APC/CY5.5 (SB Cat. 1540-18) and Rat Anti-Mouse CD8α-FITC (SB Cat. No. 1550-02).

BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD4-APC/CY5.5 (SB Cat. 1540-18) and Rat Anti-Mouse CD8α-FITC (SB Cat. No. 1550-02).
 
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References
1. Dialynas DP, Quan ZS, Wall KA, Pierres A, Quintáns J, Loken MR, et al. Characterization of the murine T cell surface molecule, designated L3T4, identified by monoclonal antibody GK1.5: similarity of L3T4 to the human Leu-3/T4 molecule. J Immunol. 1983;131:2445-51. (Immunogen, FC, IP, Depletion, Block)
2. Delong P, Tanaka T, Kruklitis R, Henry AC, Kapoor V, Kaiser LR, et al. Use of cyclooxygenase-2 inhibition to enhance the efficacy of immunotherapy. Cancer Res. 2003;63:7845-52. (IHC-FS)
3. Xia RH, Yosef N, Ubogu EE. Clinical, electrophysiological and pathologic correlations in a severe murine experimental autoimmune neuritis model of Guillain-Barré syndrome. J Neuroimmunol. 2010;219:54-63. (IHC-FS)
4. Kosuge H, Ishihara T, Haraguchi G, Maejima Y, Okada H, Saiki H, et al. Treatment with telmisartan attenuates graft arteriosclerosis in murine cardiac allografts. J Heart Lung Transplant. 2010;29:562-7. (IHC-FS)
5. Choi J, Oh S, Lee D, Oh HJ, Park JY, Lee SB, et al. Mst1-FoxO signaling protects naïve T lymphocytes from cellular oxidative stress in mice. PloS One. 2009;4(11):e8011. (ICC, FC)
6. Alari-Pahissa E, Vega-Ramos J, Zhang J, Castaño AR, Turley SJ, Villadangos JA, et al. Differential effect of CD69 targeting on bystander and antigen-specific T cell proliferation. J Leukoc Biol. 2012;92:145-58. (Sep)
7. Grodeland G, Mjaaland S, Roux KH, Fredriksen AB, Bogen B. DNA vaccine that targets hemagglutinin to MHC class II molecules rapidly induces antibody-mediated protection against influenza. J Immunol. 2013;191:3221-31. (Depletion, FC)
8. Ye X, Robinson MB, Pabin C, Batshaw ML, Wilson JM. Transient depletion of CD4 lymphocyte improves efficacy of repeated administration of recombinant adenovirus in the ornithine transcarbamylase deficient sparse fur mouse. Gene Ther. 2000;7:1761-7. (Depletion, Block)
9. Travers H, Anderson G, Gentle D, Jenkinson E, Girdlestone J. Protocols for high efficiency, stage-specific retroviral transduction of murine fetal thymocytes and thymic epithelial cells. J Immunol Methods. 2001;253:209-22. (Sep)
10. Vega-Ramos J, Alari-Pahissa E, del Valle J, Carrasco-Marín E, Esplugues E, Borràs M, et al. CD69 limits early inflammatory diseases associated with immune response to Listeria monocytogenes infection. Immunol Cell Biol. 2010;88:707-15. (Sep)
11. Avagyan S, Glouchkova L, Choi J, Snoeck H. A quantitative trait locus on chromosome 4 affects cycling of hematopoietic stem and progenitor cells through regulation of TGF-β2 responsiveness. J Immunol. 2008;181:5904-11. (FC)
12. Barnes MJ, Krebs P, Harris N, Eidenschenk C, Gonzalez-Quintal R, Arnold CN, et al. Commitment to the regulatory T cell lineage requires CARMA1 in the thymus but not in the periphery. PloS Biol. 2009;7(3):e1000051. (FC)
13. Safari D, Dekker HA, Rijkers G, Snippe H. Codelivery of adjuvants at the primary immunization site is essential for evoking a robust immune response to neoglycoconjugates. Vaccine. 2011;29:849-54. (FC)
14. Berge T, Grønningsæter IH, Lorvik KB, Abrahamsen G, Granum S, Sundvold-Gjerstad V, et al. SH2D2A modulates T cell mediated protection to a B cell derived tumor in transgenic mice. PLoS One. 2012;7(10):e48239. (FC)
15. Jacobsen J, Haabeth OW, Tveita AA, Schjetne KW, Munthe LA, Bogen B. Naive idiotope-specific B and T cells collaborate efficiently in the absence of dendritic cells. J Immunol. 2014;192:4174-83. (FC)
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