Rat Anti-Mouse CD86-Alexa Fluor® 700

Cat. No.
0.1 mg  
Price (USD)
Clone GL1
Isotype Rat (LOU) IgG2aκ
Isotype Control Rat IgG2a-Alexa Fluor® 700
Immunogen LPS-activated CBA/Ca mouse spleen B cells
Specificity Mouse CD86
Alternalte Name(s) B7-2, B70, Ly-58
Description CD86, also known as B7-2, is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by B-cell specific stimuli with peak expression at 18-42 hours after stimulation. CD86, along with CD80 (B7-1), is an important accessory molecule in T cell costimulation via its interaction with CD28 and CD152 (CTLA-4). Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. The monoclonal antibody GL1 blocks mixed lymphocyte reactions and stimulation of T cells by antigen-presenting cells.
Format/Conjugate AF700 (Alexa Fluor® 700)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 0.2 mL
Storage & Handling 2-8°C; Avoid exposure to light
Please refer to product specific SDS
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 1,3-13
Immunohistochemistry-Frozen Sections – Reported in literature 4
Immunoprecipitation – Reported in literature 1
Blocking – Reported in literature 1,3,4
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795220
Gene ID
12524 (Mouse)
Gene Symbol
Cd86 (Mouse)
Gene Aliases B7; B70; MB7; B7-2; B7.2; CLS1; Ly58; ETC-1; Ly-58; MB7-2; Cd28l2; TS/A-2
Uniprot ID
P42082 (Mouse)
Uniprot Name
  • DBA/2 mouse mastocytoma cell line P815 and mB7.2-Ig transfected P815 cells were stained with Rat Anti-Mouse CD86-AF700 (SB Cat. No. 1735-27).

DBA/2 mouse mastocytoma cell line P815 and mB7.2-Ig transfected P815 cells were stained with Rat Anti-Mouse CD86-AF700 (SB Cat. No. 1735-27).
Related Products (6)
1. Hathcock KS, Laszlo G, Dickler HB, Bradshaw J, Linsley P, Hodes RJ. Identification of an alternative CTLA-4 ligand costimulatory for T cell activation. Science. 1993;262:905-7. (Immunogen, IP, FC, Block)
2. Laszlo G, Hathcock KS, Dickler HB, Hodes RJ. Characterization of a novel cell-surface molecule expressed on subpopulations of activated T and B cells. J Immunol. 1993;150:5252-62. (Immunogen)
3. Hathcock KS, Laszlo G, Pucillo C, Linsley P, Hodes RJ. Comparative analysis of B7-1 and B7-2 costimulatory ligands: expression and function. J Exp Med. 1994;180:631-40. (FC, Block)
4. Inaba K, Witmer-Pack M, Inaba M, Hathcock KS, Sakuta H, Azuma M, et al. The tissue distribution of the B7-2 costimulator in mice: abundant expression on dendritic cells in situ and during maturation in vitro. J Exp Med. 1994;180:1849-60. (FC, Block, IHC-FS)
5. Cheng X, Wang C, Qian G, Zhu B. CD80, but not CD86 were up-regulated on the spleen-derived dendritic cells from OVA-sensitized and challenged BALB/c mice. Immunol Lett. 2003;89:31-38. (FC)
6. Choi K, Kim J, Lee Y, Kim J, Suh B, Kim H, et al. Concurrent delivery of GM-CSF and B7-1 using an oncolytic adenovirus elicits potent antitumor effect. Gene Ther. 2006;13:1010-20. (FC)
7. Lee Y, Kim J, Choi K, Choi I, Kim H, Cho S, et al. Enhanced antitumor effect of oncolytic adenovirus expressing interleukin-12 and B7-1 in an immunocompetent murine model. Clin Cancer Res. 2006;12:5859-68. (FC)
8. Zaru R, Mollahan P, Watts C. 3-phosphoinositide-dependent kinase 1 deficiency perturbs toll-like receptor signaling events and actin cytoskeleton dynamics in dendritic cells. J Biol Chem. 2008;283:929-39. (FC)
9. Fink LN, Frøkiær H. Dendritic cells from Peyer's patches and mesenteric lymph nodes differ from spleen dendritic cells in their response to commensal gut bacteria. Scand J Immunol. 2008;68:270-9. (FC)
10. Wang Y, Bai C, Wang G, Wang D, Cheng X, Huang J, et al. Protection against the allergic airway inflammation depends on the modulation of spleen dendritic cell function and induction of regulatory T cells in mice. Genet Vaccines Ther. 2010;8:2. (FC)
11. Jacobsen JT, Lunde E, Sundvold-Gjerstad V, Munthe LA, Bogen B. The cellular mechanism by which complementary Id+ and anti-Id antibodies communicate: T cells integrated into idiotypic regulation. Immunol Cell Biol. 2010;88:515-22. (FC)
12. Wismar R, Brix S, Lærke HN, Frøkiær H. Comparative analysis of a large panel of non-starch polysaccharides reveals structures with selective regulatory properties in dendritic cells. Mol Nutr Food Res. 2011;55:443-54. (FC)
13. Li J, Liu Y, Gao X, Gao X, Cai H. TLR2 and TLR4 signaling pathways are required for recombinant Brucella abortus BCSP31-induced cytokine production, functional upregulation of mouse macrophages, and the Th1 immune response in vivo and in vitro. Cell Mol Immunol. 2014;11:477-94. (FC)
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