Mouse Anti-Mouse I-Ab-PE

Cat. No.
1896-09  
Size
0.1 mg  
Price (USD)
$215.00 
Clone 25-9-3
Isotype Mouse (C3H) IgMκ
Isotype Control Mouse IgM-PE
Immunogen C3H.SW mouse splenocytes
Specificity Mouse I-Ab
Alternalte Name(s) MHC Class II
Description The monoclonal antibody 25-9-3 reacts specifically with the I-Ab haplotype of MHC Class II molecules. Class II antigens are predominantly expressed on antigen-presenting cells including B lymphocytes, macrophages, dendritic cells, and certain epithelial cells.
Format/Conjugate PE (R-phycoerythrin)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide and a stabilizer
Concentration 0.1 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C; Avoid exposure to light; Do not freeze
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested
Immunohistochemistry-Frozen Sections – Reported in literature 2
Complement Mediated Cell Depletion – Reported in literature 3-6
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795451
Gene ID
14961 (Mouse)
Gene Symbol
H2-Ab1 (Mouse)
Gene Aliases AI845868; Abeta; H-2Ab; H2-Ab; I-Abeta; IAb; Ia-2; Ia2; Rmcs1
Uniprot ID
P14483 (Mouse)
Uniprot Name
  • C57BL/6 mouse splenocytes were stained with Mouse Anti-Mouse I-Ab-PE (SB Cat. 1896-09) and Rat Anti-Mouse CD3ε-FITC (SB Cat. No. 1535-02)

C57BL/6 mouse splenocytes were stained with Mouse Anti-Mouse I-Ab-PE (SB Cat. 1896-09) and Rat Anti-Mouse CD3ε-FITC (SB Cat. No. 1535-02)
 
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References
1. Ozato K, Sachs DH. Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression. J Immunol. 1981;126:317-21. (Immunogen)
2. Komano H, Fujiura Y, Kawaguchi M, Matsumoto S, Hashimoto Y, Obana S, et al. Homeostatic regulation of intestinal epithelia by intraepithelial γδ T cells. Proc Natl Acad Sci USA. 1995;92:6147-51. (IHC-FS)
3. Sperling AI, Green JM, Mosley RL, Smith PL, DiPaolo RJ, Klein JR, et al. CD43 is a murine T cell costimulatory receptor that functions independently of CD28. J Exp Med. 1995;182:139-46. (CMDC)
4. Lenschow DJ, Sperling AI, Cooke MP, Freeman G, Rhee L, Decker DC, et al. Differential up-regulation of the B7-1 and B7-2 costimulatory molecules after Ig receptor engagement by antigen. J Immunol. 1994;153:1990-7. (CMDC)
5. Khattri R, Auger JA, Griffin MD, Sharpe AH, Bluestone JA. Lymphoproliferative disorder in CTLA-4 knockout mice is characterized by CD28-regulated activation of Th2 responses. J Immunol. 1999;162:5784-91. (CMDC)
6. Rulifson IC, Szot GL, Palmer E, Bluestone JA. Inability to induce tolerance through direct antigen presentation. Am J Transplant. 2002;2:510-9. (CMDC)