Mouse Anti-Human CD11b-FITC

Cat. No.
9546-02  
Size
100 tests  
Price (USD)
$180.00 
Clone ICRF44
Isotype Mouse (BALB/c) IgG1κ
Isotype Control Mouse IgG1-FITC
Immunogen Rheumatoid synovial cells and human monocytes
Specificity Human/Porcine/Rhesus/Cynomolgus/Chimpanzee/Baboon CD11b
Alternalte Name(s) Mac-1α, Mo-1, CR3, integrin αM, C3biR, ITGAM, CR3A, p170
Description CD11b is the 165 kDa αM chain which combines with the CD18 β2 integrin subunit to form the Mac-1 heterodimer. It is expressed on activated lymphocytes, monocytes, granulocytes and a subset of NK cells. CD11b functions in cell-cell and cell-substrate interactions and is a ligand for C3bi, CD50 (ICAM-3), CD54 (ICAM-1) and CD102 (ICAM-2).
Format/Conjugate FITC (Fluorescein)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide
Volume 1.0 mL
Storage & Handling 2-8°C; Avoid exposure to light
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 7,9
Immunohistochemistry-Frozen Sections – Reported in literature 3
Immunocytochemistry – Reported in literature 4
Immunoprecipitation – Reported in literature 1
Western Blot – Reported in literature 2
Blocking – Reported in literature 5,6
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2796901
Gene ID
3684 (Human)
397459 (Porcine)
714832 (Rhesus)
101866035 (Cynomolgus)
454069 (Chimpanzee)
101004087 (Baboon)
Gene Symbol
ITGAM (Human)
ITGAM (Porcine)
ITGAM (Rhesus)
ITGAM (Cynomolgus)
ITGAM (Chimpanzee)
ITGAM (Baboon)
Gene Aliases CD11B; CR3A; MAC-1; MAC1A; MO1A; SLEB6; CK820_G0050589
Uniprot ID
P11215 (Human)
Uniprot Name
  • Human peripheral blood granulocytes were stained with Mouse Anti-Human CD11b-FITC (SB Cat. No. 9546-02).

Human peripheral blood granulocytes were stained with Mouse Anti-Human CD11b-FITC (SB Cat. No. 9546-02).
 
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References
1. Feng C, Zhang L, Almulki L, Faez S, Whitford M, Hafezi-Moghadam A, et al. Endogenous PMN sialidase activity exposes activation epitope on CD11b/CD18 which enhances its binding interaction with ICAM-1. J Leukoc Biol. 2011;90:313-21. (IP)
2. David A, Kacher Y, Specks U, Aviram I. Interaction of proteinase 3 with CD11b/CD18 (β2 integrin) on the cell membrane of human neutrophils. J Leukoc Biol. 2003;74:551-7. (WB)
3. Mayer WJ, Klaproth OK, Hengerer FH, Kook D, Dirisamer M, Priglinger S, et al. In vitro immunohistochemical and morphological observations of penetrating corneal incisions created by a femtosecond laser used for assisted intraocular lens surgery. J Cataract Refract Surg. 2014;40:632-8. (IHC-FS)
4. Friedenberger M, Bode M, Krusche A, Schubert W. Fluorescence detection of protein clusters in individual cells and tissue sections by using toponome imaging system: sample preparation and measuring procedures. Nat Protoc. 2007;2:2285-94. (ICC)
5. Malhotra V, Hogg N, Sim RB. Ligand binding by the p150,95 antigen of U937 monocytic cells: properties in common with complement receptor type 3 (CR3). Eur J Immunol. 1986;16:1117-23. (Block)
6. Nasr AB, Haithcoat J, Masterson JE, Gunn JS, Eaves-Pyles T, Klimpel GR. Critical role for serum opsonins and complement receptors CR3 (CD11b/CD18) and CR4 (CD11c/CD18) in phagocytosis of Francisella tularensis by human dendritic cells (DC): uptake of Francisella leads to activation of immature DC and intracellular survival of the bacteria. J Leukoc Biol. 2006;80:774-86. (Block)
7. Lester EA, Babensee JE. Proinflammatory phenotype of endothelial cells after coculture with biomaterial-treated blood cells. J Biomed Mater Res A. 2003;64:397-410. (FC)
8. Hérodin F, Thullier P, Garin M, Drouet M. Nonhuman primates are relevant models for research in hematology, immunology and virology. Eur Cytokine Netw. 2005;16:104-16. (Chimpanzee, Baboon, Cynomolgus, Rhesus Reactivity)
9. Singh S, Kloss FR, Brunauer R, Schimke M, Jamnig A, Greiderer-Kleinlercher B, et al. Mesenchymal stem cells show radioresistance in vivo. J Cell Mol Med. 2012;16:877-87. (FC, Porcine Reactivity)