Neutralite Avidin-HRP

Cat. No.
7200-05  
Size
1.0 mL  
Price (USD)
$115.00 
Source Neutralized (pI = 6.3 ± 0.3), deglycosylated avidin purified from egg white
Specificity Biotin
Format/Conjugate HRP (Horseradish Peroxidase)
Buffer Formulation 50% Glycerol/50% Phosphate buffered saline, pH 7.4
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications ELISA – Quality tested 1-9
FLISA – Quality tested
Flow Cytometry – Quality tested 2,10,12-14
ELISpot – Reported in literature 3,10,11
Immunohistochemistry-Frozen Sections – Reported in literature 10,15-18
Immunohistochemistry-Paraffin Sections – Reported in literature 19
Immunocytochemistry – Reported in literature 20,21
Western Blot – Reported in literature 4,22-24
Microarray – Reported in literature 25
Recommended Dilutions Please refer to product specific Technical Bulletin
  • ELISA plate was coated with Goat Anti-Human IgG-BIOT (SB Cat. No. 2040-08) and purified/unlabeled Rat IgG2bκ. Biotin conjugated antibody and purified immunoglobulin were detected with serially diluted Neutralite Avidin-HRP (SB Cat. No. 7200-05).

ELISA plate was coated with Goat Anti-Human IgG-BIOT (SB Cat. No. 2040-08) and purified/unlabeled Rat IgG2bκ. Biotin conjugated antibody and purified immunoglobulin were detected with serially diluted Neutralite Avidin-HRP (SB Cat. No. 7200-05).
 
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References
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2. Reinagel ML, Taylor RP. Transfer of immune complexes from erythrocyte CR1 to mouse macrophages. J Immunol. 2000;164:1977-85. (ELISA, FC)
3. Hodge LM, Marinaro M, Jones HP, McGhee JR, Kiyono H, Simecka JW. Immunoglobulin A (IgA) responses and IgE-associated inflammation along the respiratory tract after mucosal but not systemic immunization. Infect Immun. 2001;69:2328-38. (ELISA, ELISPOT)
4. Wang S, Hirschberg R. BMP7 antagonizes TGF-β-dependent fibrogenesis in mesangial cells. Am J Physiol Renal Physiol. 2003;284:1006-13. (ELISA, WB)
5. Darniot M, Pitoiset C, Petrella T, Aho S, Pothier P, Manoha C. Age-associated aggravation of clinical disease after primary metapneumovirus infection of BALB/c mice. J Virol. 2009;83:3323-32. (ELISA)
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7. Weinstein JS, Delano MJ, Xu Y, Kelly-Scumpia KM, Nacionales DC, Li Y, et al. Maintenance of anti-Sm/RNP autoantibody production by plasma cells residing in ectopic lymphoid tissue and bone marrow memory B cells. J Immunol. 2013;190:3916-27. (ELISA)
8. Buckner LR, Amedee AM, Albritton HL, Kozlowski PA, Lacour N, McGowin CL, et al. Chlamydia trachomatis infection of endocervical epithelial cells enhances early HIV transmission events. PLoS One. 2016;11(1):e0146663. (ELISA)
9. Odeh AN, Simecka JW. Regulatory CD4+CD25+ T cells dampen inflammatory disease in murine mycoplasma pneumonia and promote IL-17 and IFN-γ responses. PLoS One. 2016;11(5):e0155648. (ELISA)
10. van Ginkel FW, Gulley SL, Lammers A, Hoerr FJ, Gurjar R, Toro H. Conjunctiva-associated lymphoid tissue in avian mucosal immunity. Dev Comp Immunol. 2012;36:289-97. (ELISPOT, FC, IHC-FS)
11. Latham LB, Lee MJ, Lincoln JA, Ji N, Forsthuber TG, Lindsey JW. Antivirus immune activity in multiple sclerosis correlates with MRI activity. Acta Neurol Scand. 2016;133:17-24. (ELISPOT)
12. Bowman L, Grossmann M, Rill D, Brown M, Zhong W, Alexander B, et al. IL-2 adenovector-transduced autologous tumor cells induce antitumor immune responses in patients with neuroblastoma. Blood. 1998;92:1941-9. (FC)
13. Flynn S, Toellner K, Raykundalia C, Goodall M, Lane P. CD4 T cell cytokine differentiation: the B cell activation molecule, OX40 ligand, instructs CD4 T cells to express interleukin 4 and upregulates expression of the chemokine receptor, Blr-1. J Exp Med. 1998;188:297-304. (FC)
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15. Ettinger R, Munson SH, Chao C, Vadeboncoeur M, Toma J, McDevitt HO. A critical role for lymphotoxin-β receptor in the development of diabetes in nonobese diabetic mice. J Exp Med. 2001;193:1333-9. (IHC-FS)
16. Bénard A, Ceredig R, Rolink AG. Regulatory T cells control autoimmunity following syngeneic bone marrow transplantation. Eur J Immunol. 2006;36:2324-35. (IHC-FS)
17. van Ginkel FW, Tang DC, Gulley SL, Toro H. Induction of mucosal immunity in the avian Harderian gland with a replication-deficient Ad5 vector expressing avian influenza H5 hemagglutinin. Dev Comp Immunol. 2009;33:28-34. (IHC-FS)
18. Pérez CV, Pellizzari EH, Cigorraga SB, Galardo MN, Naito M, Lustig L, et al. IL17A impairs blood-testis barrier integrity and induces testicular inflammation. Cell Tissue Res. 2014;358:885-98. (IHC-FS)
19. Kivisäkk P, Mahad DJ, Callahan MK, Sikora K, Trebst C, Tucky B, et al. Expression of CCR7 in multiple sclerosis: implications for CNS immunity. Ann Neurol. 2004;55:627-38. (IHC-PS)
20. Wong CW, Wiedle G, Ballestrem C, Wehrle-Haller B, Etteldorf S, Bruckner M, et al. PECAM-1/CD31 trans-homophilic binding at the intercellular junctions is independent of its cytoplasmic domain; evidence for heterophilic interaction with integrin αvβ3 in Cis. Mol Biol Cell. 2000;11:3109-21. (ICC)
21. Sorbarzo CM, Rosana Nde M, Livia L, Berta D, Schteingart HF. Mono-(2-ethylhexyl) phthalate (MEHP) affects intercellular junctions of Sertoli cell: A potential role of oxidative stress. Reprod Toxicol. 2015;58:203-12. (ICC)
22. Clancy JP, Ruiz FE, Sorscher EJ. Adenosine and its nucleotides activate wild-type and R117H CFTR through an A2B receptor-coupled pathway. Am J Physiol. 1999;276:C361-9. (WB)
23. Bresalier RS, Byrd JC, Tessler D, Lebel J, Koomen J, Hawke D, et al. A circulating ligand for galectin-3 is a haptoglobin-related glycoprotein elevated in individuals with colon cancer. Gastroenterology. 2004;127:741-8. (WB)
24. Polyak MJ, Li H, Shariat N, Deans JP. CD20 homo-oligomers physically associate with the B cell antigen receptor. Dissociation upon receptor engagement and recruitment of phosphoproteins and calmodulin-binding proteins. J Biol Chem. 2008;283:18545-52. (WB)
25. Jeon M, Kang M, Park KH. 7-Triazolylcoumarin-based fluorescent tag system for stepwise, comparative assessment of small molecule microarrays. Tetrahedron. 2012;68:6038-53. (Microarray)