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Human IgG1 Kappa-LE/AF

Cat. No.
0151K-14  
Size
0.5 mg  
Price (USD)
$120.00 
Qty

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Source Human myeloma serum
Format/Conjugate LE/AF (Low Endotoxin/Azide Free)
Buffer Formulation Supplied in PBS
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling Store at -20°C
Please refer to product specific (M)SDS
Applications
Quality tested applications for relevant formats include -
ELISA 1,2
FLISA
Other referenced applications for relevant formats include -
Flow Cytometry 5
Immunoprecipitation 3
In vitro Control 4
In vivo Control 1
Surface Plasmon Resonance 1
Recommended Dilutions Please refer to product specific Technical Bulletin
  • ELISA plate was coated with Human IgG1 Kappa-UNLB (SB Cat. No. 0151K-01). Immunoglobulin was detected with serially diluted Mouse Anti-Human IgG1 Hinge-BIOT (SB Cat. No. 9052-08), Mouse Anti-Human IgG2 Fc-BIOT (SB Cat. No. 9060-08), Mouse Anti-Human IgG3 Hinge-BIOT (SB Cat. No. 9210-08), Mouse Anti-Human IgG4 pFc’-BIOT (SB Cat. No. 9190-08), Mouse Anti-Human IgA1-BIOT (SB Cat. No. 9130-08), Mouse Anti-Human IgA2-BIOT (SB Cat. No. 9140-08), Mouse Anti-Human IgD-BIOT (SB Cat. No. 9030-08), Mouse Anti-Human IgE Fc-BIOT (SB Cat. No. 9160-08), Mouse Anti-Human IgM-BIOT (SB Cat. No. 9020-08), Mouse Anti-Human Kappa-BIOT (SB Cat. No. 9230-08), and Mouse Anti-Human Lambda-BIOT (SB Cat. No. 9180-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).

ELISA plate was coated with Human IgG1 Kappa-UNLB (SB Cat. No. 0151K-01). Immunoglobulin was detected with serially diluted Mouse Anti-Human IgG1 Hinge-BIOT (SB Cat. No. 9052-08), Mouse Anti-Human IgG2 Fc-BIOT (SB Cat. No. 9060-08), Mouse Anti-Human IgG3 Hinge-BIOT (SB Cat. No. 9210-08), Mouse Anti-Human IgG4 pFc’-BIOT (SB Cat. No. 9190-08), Mouse Anti-Human IgA1-BIOT (SB Cat. No. 9130-08), Mouse Anti-Human IgA2-BIOT (SB Cat. No. 9140-08), Mouse Anti-Human IgD-BIOT (SB Cat. No. 9030-08), Mouse Anti-Human IgE Fc-BIOT (SB Cat. No. 9160-08), Mouse Anti-Human IgM-BIOT (SB Cat. No. 9020-08), Mouse Anti-Human Kappa-BIOT (SB Cat. No. 9230-08), and Mouse Anti-Human Lambda-BIOT (SB Cat. No. 9180-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
 
References
1. Bock F, Onderka J, Dietrich T, Bachmann B, Kruse FE, Paschke M, et al. Bevacizumab as a potent inhibitor of inflammatory corneal angiogenesis and lymphangiogenesis. Invest Ophthalmol Vis Sci. 2007;48:2545-52. (ELISA, in vivo control, Surface plasmon resonance)
2. Zhang J, Jacobi AM, Wang T, Berlin R, Volpe BT, Diamond B. Polyreactive autoantibodies in systemic lupus erythematosus have pathogenic potential. J Autoimmun. 2009;33:270-4. (ELISA)
3. Yang G, Holl TM, Liu Y, Li Y, Lu X, Nicely NI, et al. Identification of autoantigens recognized by the 2F5 and 4E10 broadly neutralizing HIV-1 antibodies. J Exp Med. 2013;210:241-56. (IP)
4. Ackler S, Mitten MJ, Foster K, Oleksijew A, Refici M, Tahir SK, et al. The Bcl-2 inhibitor ABT-263 enhances the response of multiple chemotherapeutic regimens in hematologic tumors in vivo. Cancer Chemother Pharmacol. 2010;66:869-80. (In vitro control)
5. Hotard AL, Lee S, Currier MG, Crowe JE Jr, Sakamoto K, Newcomb DC, et al. Identification of residues in the human respiratory syncytial virus fusion protein that modulate fusion activity and pathogenesis. J Virol. 2015;89:512-22. (FC)
6. Liu H, Chumsae C, Gaza-Bulseco G, Hurkmans K, Radziejewski CH. Ranking the susceptibility of disulfide bonds in human IgG1 antibodies by reduction, differential alkylation, and LC-MS analysis. Anal Chem. 2010;82:5219-26. (Mass spectrometry)
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