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Goat Anti-Mouse IgG2a, Human ads-APC

Cat. No.
1080-11L  
Size
0.5 mg  
Price (USD)
$425.00 
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Specificity Reacts with the heavy chain of mouse IgG2a as demonstrated by ELISA, FLISA, and/or flow cytometry. Minimal cross reactivity with human immunoglobulins. May react with immunoglobulins from other species.
Source Pooled antisera from goats hyperimmunized with mouse IgG2a paraproteins
Cross Adsorption Mouse IgM, IgG1, IgG2b, IgG3 and IgA; pooled human sera; purified human paraproteins
Purification Affinity chromatography on mouse IgG2a covalently linked to agarose
Isotype Goat IgG
Isotype Control Goat IgG-APC
Format/Conjugate APC (Allophycocyanin)
Buffer Formulation Supplied in PBS/NaN3 and a stabilizing agent
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling Store at 2-8°C
Please refer to product specific (M)SDS
Applications
Quality tested applications for relevant formats include -
ELISA
FLISA
Flow Cytometry
Other referenced applications for relevant formats include -
Immunohistochemistry-Frozen Sections
Immunohistochemistry-Paraffin Sections
Immunocytochemistry
Immunoblotting
ELISpot
Purification
Recommended Dilutions Please refer to product specific Technical Bulletin
  • FLISA plate was coated with purified mouse IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG2a, Human ads-APC (SB Cat. No. 1080-11L).
  • Human blood peripheral lymphocytes were stained with Mouse Anti-Human CD5-UNLB followed by Goat Anti-Mouse IgG2a, Human ads-APC (Cat. No. 1080-11L).

FLISA plate was coated with purified mouse IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG2a, Human ads-APC (SB Cat. No. 1080-11L).
 

Human blood peripheral lymphocytes were stained with Mouse Anti-Human CD5-UNLB followed by Goat Anti-Mouse IgG2a, Human ads-APC (Cat. No. 1080-11L).
 
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