Goat Anti-Human IgM-Alexa Fluor® 647

Cat. No.
2020-31  
Size
1.0 mg  
Price (USD)
$115.00 
Specificity Reacts with the heavy chain of human IgM
Source Pooled antisera from goats hyperimmunized with human IgM
Cross Adsorption Human IgG and IgA; may react with IgM from other species
Purification Affinity chromatography on human IgM covalently linked to agarose
Isotype Goat IgG
Isotype Control Goat IgG-Alexa Fluor® 647
Format/Conjugate AF647 (Alexa Fluor® 647)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide
Concentration 1.0 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C; Avoid exposure to light
Please refer to product specific SDS
Applications
Quality tested applications for relevant formats include -
ELISA 1-3,15
FLISA
Flow Cytometry 16-18
Other referenced applications for relevant formats include -
ELISpot 2,4,5,11
Immunohistochemistry-Frozen Sections 6,7
Immunohistochemistry-Paraffin Sections 8,9
Immunocytochemistry 8,10,11
Western Blot 12,13
Immunoprecipitation 13-15
Multiplex 19,20
Stimulation 21-23
Purification 24
Depletion 25
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795608
  • FLISA plate was coated with purified human IgM, IgG, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Human IgM-AF647 (SB Cat. No. 2020-31).
  • Human peripheral blood lymphocytes were stained with Goat Anti-Human IgM-AF647 (SB Cat. 2020-31) and Mouse Anti-Human CD19-FITC (SB Cat. No. 9340-02).

FLISA plate was coated with purified human IgM, IgG, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Human IgM-AF647 (SB Cat. No. 2020-31).
 

Human peripheral blood lymphocytes were stained with Goat Anti-Human IgM-AF647 (SB Cat. 2020-31) and Mouse Anti-Human CD19-FITC (SB Cat. No. 9340-02).
 
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References
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