Goat Anti-Human IgM-Alexa Fluor® 647

Cat. No.
1.0 mg  
Price (USD)
Specificity Reacts with the heavy chain of human IgM
Source Pooled antisera from goats hyperimmunized with human IgM
Cross Adsorption Human IgG and IgA; may react with IgM from other species
Purification Affinity chromatography on human IgM covalently linked to agarose
Isotype Goat IgG
Isotype Control Goat IgG-Alexa Fluor® 647
Format/Conjugate AF647 (Alexa Fluor® 647)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide
Concentration 1.0 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C; Avoid exposure to light
Please refer to product specific SDS
Quality tested applications for relevant formats include -
ELISA 1-3,15
Flow Cytometry 16-18
Other referenced applications for relevant formats include -
ELISpot 2,4,5,11
Immunohistochemistry-Frozen Sections 6,7
Immunohistochemistry-Paraffin Sections 8,9
Immunocytochemistry 8,10,11
Western Blot 12,13
Immunoprecipitation 13-15
Multiplex 19,20
Stimulation 21-23
Purification 24
Depletion 25
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795608
  • FLISA plate was coated with purified human IgM, IgG, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Human IgM-AF647 (SB Cat. No. 2020-31).
  • Human peripheral blood lymphocytes were stained with Goat Anti-Human IgM-AF647 (SB Cat. 2020-31) and Mouse Anti-Human CD19-FITC (SB Cat. No. 9340-02).

FLISA plate was coated with purified human IgM, IgG, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Human IgM-AF647 (SB Cat. No. 2020-31).

Human peripheral blood lymphocytes were stained with Goat Anti-Human IgM-AF647 (SB Cat. 2020-31) and Mouse Anti-Human CD19-FITC (SB Cat. No. 9340-02).
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2. Traggiai E, Volpi S, Schena F, Gattorno M, Ferlito F, Moretta L, et al. Bone marrow-derived mesenchymal stem cells induce both polyclonal expansion and differentiation of B cells isolated from healthy donors and systemic lupus erythematosus patients. Stem Cells. 2008;26:562-9. (ELISA, ELISPOT)
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7. Forshammar J, Isaksson S, Strid H, Stotzer P, Sjövall H, Simrén M, et al. A pilot study of colonic B cell pattern in irritable bowel syndrome. Scand J Gastroenterol. 2008;43:1461-6. (IHC-FS)
8. Solvason N, Kearney JF. The human fetal omentum: a site of B cell generation. J Exp Med. 1992;175:397-404. (IHC-PS, ICC)
9. Rowley AH, Shulman ST, Mask CA, Finn LS, Terai M, Baker SC, et al. IgA plasma cell infiltration of proximal respiratory tract, pancreas, kidney, and coronary artery in acute Kawasaki disease. J Infect Dis. 2000;182:1183-91. (IHC-PS)
10. Campana D, Farrant J, Inamdar N, Webster AD, Janossy G. Phenotypic features and proliferative activity of B cell progenitors in X-linked agammaglobulinemia. J Immunol. 1990;145:1675-80. (ICC)
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