Goat Anti-Rat IgG(H+L), Mouse ads-BIOT

Cat. No.
3050-08  
Size
1.0 mg  
Price (USD)
$165.00 
Specificity Reacts with the heavy and light chains of rat IgG and the light chains of rat IgM
Source Pooled antisera from goats hyperimmunized with rat IgG
Cross Adsorption Mouse immunoglobulins and pooled sera; may react with immunoglobulins from other species
Purification Affinity chromatography on pooled rat IgG covalently linked to agarose
Isotype Goat IgG
Isotype Control Goat IgG-BIOT
Format/Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 2.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Quality tested applications for relevant formats include -
ELISA 1-4
FLISA
Flow Cytometry 5-10
Other referenced applications for relevant formats include -
Immunohistochemistry-Frozen Sections 11-17
Immunohistochemistry-Paraffin Sections 18-20
Imuunocytochemistry 21,22
Western Blot 23-25
Separation 4
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795833
  • Frozen BALB/c mouse intestine tissue was stained with Rat IgG2a-UNLB isotype control (SB Cat. No. 0117-01; left) and Rat Anti-Mouse CD104-UNLB (SB Cat. No. 1855-01; right) followed by Goat Anti-Rat IgG(H+L), Mouse ads-BIOT (SB Cat. No. 3050-08), Streptavidin-FITC (SB Cat. No. 7100-02), and DAPI.
  • C57BL/6 mouse splenocytes were stained with Rat Anti-Mouse CD45-UNLB (SB Cat. No. 1660-01) followed by Goat Anti-Rat IgG(H+L), Mouse ads-BIOT (SB Cat. No. 3050-08) followed by Streptavidin-FITC (SB Cat. No. 7100-02).
  • Paraffin embedded mouse adductor muscle day 3 post femoral artery ligation was stained with anti-Mac-3 followed by Goat Anti-Rat IgG(H+L), Mouse ads-BIOT (SB Cat. No. 3050-08), Streptavidin-HRP (SB Cat. No. 7100-05), hematoxylin, and AEC.

    Image from van den Borne P, Rygiel TP, Hoogendoorn A, Westerlaken GH, Boon L, Quax PH, et al. The CD200-CD200 receptor inhibitory axis controls arteriogenesis and local T lymphocyte influx. PLoS One. 2014;9(6):e98820. Figure 2(f)
  • Paraffin embedded mouse adductor muscle day 7 post femoral artery ligation was stained with anti-Mac-3 followed by Goat Anti-Rat IgG(H+L), Mouse ads-BIOT (SB Cat. No. 3050-08), Streptavidin-HRP (SB Cat. No. 7100-05), hematoxylin, and AEC.

    Image from van den Borne P, Rygiel TP, Hoogendoorn A, Westerlaken GH, Boon L, Quax PH, et al. The CD200-CD200 receptor inhibitory axis controls arteriogenesis and local T lymphocyte influx. PLoS One. 2014;9(6):e98820. Figure 2(f)

Frozen BALB/c mouse intestine tissue was stained with Rat IgG2a-UNLB isotype control (SB Cat. No. 0117-01; left) and Rat Anti-Mouse CD104-UNLB (SB Cat. No. 1855-01; right) followed by Goat Anti-Rat IgG(H+L), Mouse ads-BIOT (SB Cat. No. 3050-08), Streptavidin-FITC (SB Cat. No. 7100-02), and DAPI.
 

C57BL/6 mouse splenocytes were stained with Rat Anti-Mouse CD45-UNLB (SB Cat. No. 1660-01) followed by Goat Anti-Rat IgG(H+L), Mouse ads-BIOT (SB Cat. No. 3050-08) followed by Streptavidin-FITC (SB Cat. No. 7100-02).
 

Paraffin embedded mouse adductor muscle day 3 post femoral artery ligation was stained with anti-Mac-3 followed by Goat Anti-Rat IgG(H+L), Mouse ads-BIOT (SB Cat. No. 3050-08), Streptavidin-HRP (SB Cat. No. 7100-05), hematoxylin, and AEC.

Image from van den Borne P, Rygiel TP, Hoogendoorn A, Westerlaken GH, Boon L, Quax PH, et al. The CD200-CD200 receptor inhibitory axis controls arteriogenesis and local T lymphocyte influx. PLoS One. 2014;9(6):e98820. Figure 2(f)
 

Paraffin embedded mouse adductor muscle day 7 post femoral artery ligation was stained with anti-Mac-3 followed by Goat Anti-Rat IgG(H+L), Mouse ads-BIOT (SB Cat. No. 3050-08), Streptavidin-HRP (SB Cat. No. 7100-05), hematoxylin, and AEC.

Image from van den Borne P, Rygiel TP, Hoogendoorn A, Westerlaken GH, Boon L, Quax PH, et al. The CD200-CD200 receptor inhibitory axis controls arteriogenesis and local T lymphocyte influx. PLoS One. 2014;9(6):e98820. Figure 2(f)
 
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References
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