Goat F(ab')2 Anti-Rabbit IgG(H+L), Mouse/Human ads-Alexa Fluor® 647

Cat. No.
0.5 mg  
Price (USD)
Specificity Reacts with the heavy and light chains of rabbit IgG and the light chains of rabbit IgM
Source Pepsin digest of Goat Anti-Rabbit IgG(H+L), Mouse/Human ads (SB Cat. No. 4050)
Cross Adsorption Mouse and human immunoglobulins and pooled sera; may react with immunoglobulins from other species
Isotype Goat F(ab')2 IgG
Format/Conjugate AF647 (Alexa Fluor® 647)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C; Avoid exposure to light
Please refer to product specific SDS
Quality tested applications for relevant formats include -
Flow Cytometry 3-10
Other referenced applications for relevant formats include -
Immunohistochemistry-Frozen Sections 11-14
Immunohistochemistry-Paraffin Sections 15-17
Imuunocytochemistry 6,9,14,18-20
Western Blot 21-24
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795972
  • FLISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Goat F(ab')2 Anti-Rabbit IgG(H+L), Mouse/Human ads-AF647 (SB Cat. No. 4052-31).

FLISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Goat F(ab')2 Anti-Rabbit IgG(H+L), Mouse/Human ads-AF647 (SB Cat. No. 4052-31).
Related Products (13)
1. Pilling D, Buckley CD, Salmon M, Gomer RH. Inhibition of fibrocyte differentiation by serum amyloid P. J Immunol. 2003;171:5537-46. (ELISA)
2. Koehler NK, Stransky E, Meyer M, Gaertner S, Shing M, Schnaidt M, et al. Alpha-synuclein levels in blood plasma decline with healthy aging. PLoS One. 2015;10(4):e0123444. (ELISA)
3. Kumar S, Tarleton RL. Antigen-specific Th1 but not Th2 cells provide protection from lethal Trypanosoma cruzi infection in mice. J Immunol. 2001;166:4596-603. (FC)
4. Xi H, Kersh GJ. Sustained early growth response gene 3 expression inhibits the survival of CD4/CD8 double-positive thymocytes. J Immunol. 2004;173:340-8. (FC)
5. Fulton BE Jr, Portella M, Radke K. Dissemination of bovine leukemia virus-infected cells from a newly infected sheep lymph node. J Virol. 2006;80:7873-84. (FC)
6. Flores-Borja F, Kabouridis PS, Jury EC, Isenberg DA, Mageed RA. Altered lipid raft-associated proximal signaling and translocation of CD45 tyrosine phosphatase in B lymphocytes from patients with systemic lupus erythematosus. Arthritis Rheum. 2007;56:291-302. (FC, ICC)
7. Espinassous Q, Garcia-de-Paco E, Garcia-Verdugo I, Synguelakis M, von Aulock S, Sallenave J, et al. IL-33 enhances lipopolysaccharide-induced inflammatory cytokine production from mouse macrophages by regulating lipopolysaccharide receptor complex. J Immunol. 2009;183:1446-55. (FC)
8. Zhou Q, Yan J, Putheti P, Wu Y, Sun X, Toxavidis V, et al. Isolated CD39 expression on CD4+ T cells denotes both regulatory and memory populations. Am J Transplant. 2009;9:2303-11. (FC)
9. Crawford JR, Pilling D, Gomer RH. Improved serum-free culture conditions for spleen-derived murine fibrocytes. J Immunol Methods. 2010;363:9-20. (FC, ICC)
10. Le Gall SM, Legrand J, Benbijja M, Safya H, Benihoud K, Kanellopoulos JM, et al. Loss of P2X7 receptor plasma membrane expression and function in pathogenic B220+ double-negative T lymphocytes of autoimmune MRL/lpr mice. PLoS One. 2012;7(12):e52161. (FC)
11. Heeringa P, Van den Born J, Brouwer E, Dolman KM, Klok PA, Huitema MG, et al. Elastase, but not proteinase 3 (PR3), induces proteinuria associated with loss of glomerular basement membrane heparan sulphate after in vivo renal perfusion in rats. Clin Exp Immunol. 1996;105:321-9. (IHC-FS)
12. Pilling D, Roife D, Wang M, Ronkainen SD, Crawford JR, Travis EL, et al. Reduction of bleomycin-induced pulmonary fibrosis by serum amyloid P. J Immunol. 2007;179:4035-44. (IHC-FS)
13. Gomer RH, Pilling D, Kauvar LM, Ellsworth S, Ronkainen SD, Roife D, et al. A serum amyloid P-binding hydrogel speeds healing of partial thickness wounds in pigs. Wound Repair Regen. 2009;17:397-404. (IHC-FS)
14. Knight JS, Zhao W, Luo W, Subramanian V, O'Dell AA, Yalavarthi S, et al. Peptidylarginine deiminase inhibition is immunomodulatory and vasculoprotective in murine lupus. J Clin Invest. 2013;123:2981-93. (IHC-FS, ICC)
15. van Ginkel FW, McGhee JR, Watt JM, Campos-Torres A, Parish LA, Briles DE. Pneumococcal carriage results in ganglioside-mediated olfactory tissue infection. Proc Natl Acad Sci USA. 2003;100:14363-7. (IHC-PS)
16. Ludvigsen E, Olsson R, Stridsberg M, Janson ET, Sandler S. Expression and distribution of somatostatin receptor subtypes in the pancreatic islets of mice and rats. J Histochem Cytochem. 2004;52:391-400. (IHC-PS)
17. Hassounah NB, Nagle R, Saboda K, Roe DJ, Dalkin BL, McDermott KM. Primary cilia are lost in preinvasive and invasive prostate cancer. PLoS One. 2013;8(7):e68521. (IHC-PS)
18. Rasmussen UB, Schreiber V, Schultz H, Mischler F, Schughart K. Tumor cell-targeting by phage-displayed peptides. Cancer Gene Ther. 2002;9:606-12. (ICC)
19. Shao DD, Suresh R, Vakil V, Gomer RH, Pilling D. Pivotal Advance: Th-1 cytokines inhibit, and Th-2 cytokines promote fibrocyte differentiation. J Leukoc Biol. 2008;83:1323-33. (ICC)
20. Crawford JR, Pilling D, Gomer RH. FcγRI mediates serum amyloid P inhibition of fibrocyte differentiation. J Leukoc Biol. 2012;92:699-711. (ICC)
21. Perez-Polo JR, Reilly CB, Rea HC. Oxygen resuscitation after hypoxia ischemia stimulates prostaglandin pathway in rat cortex. Int J Dev Neurosci. 2011;29:639-44. (WB)
22. King RG, Herrin BR, Justement LB. Differential expression of the adaptor protein HSH2 controls the quantitative and qualitative nature of the humoral response. J Immunol. 2011;187:3565-77. (WB)
23. Lee-Durham JC, Wu Y, Mokkapati VU, Paulucci-Holthauzen AA, Nesic O. Induction of angiopoietin-2 after spinal cord injury. Neuroscience. 2012;202:454-64. (WB)
24. Schröder W, Goerke C, Wolz C. Opposing effects of aminocoumarins and fluoroquinolones on the SOS response and adaptability in Staphylococcus aureus. J Antimicrob Chemother. 2013;68:529-38. (WB)
Alexa Fluor® is a registered trademark of Life Technologies Corporation