Goat F(ab')2 Anti-Rabbit IgG(H+L), Mouse/Rat/Human ads-BIOT

Cat. No.
4054-08  
Size
0.5 mg  
Price (USD)
$95.00 
Specificity Reacts with the heavy and light chains of rabbit IgG and the light chains of rabbit IgM
Source Pepsin digest of Goat Anti-Rabbit IgG(H+L), Mouse/Rat/Human ads (SB Cat. No. 4049)
Cross Adsorption Mouse, rat, and human immunoglobulins and pooled sera; may react with immunoglobulins from other species
Isotype Goat F(ab')2 IgG
Isotype Control Goat F(ab')2 IgG-BIOT
Format/Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Quality tested applications for relevant formats include -
ELISA
FLISA
Flow Cytometry 1,2
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795977
  • ELISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Goat F(ab')2 Anti-Rabbit IgG(H+L), Mouse/Rat/Human ads-BIOT (SB Cat. No. 4054-08) followd by Streptavidin-HRP (SB Cat. No. 7100-05).

ELISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Goat F(ab')2 Anti-Rabbit IgG(H+L), Mouse/Rat/Human ads-BIOT (SB Cat. No. 4054-08) followd by Streptavidin-HRP (SB Cat. No. 7100-05).
 
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References
1. Huang Y, Sojka DK, Fowell DJ. Cutting edge: Regulatory T cells selectively attenuate, not terminate, T cell signaling by disrupting NF-κB nuclear accumulation in CD4 T cells. J Immunol. 2012;188:947-51. (FC)
2. Leonard A, Marando C, Rahman A, Fazal F. Thrombin selectively engages LIM kinase 1 and slingshot-1L phosphatase to regulate NF-κB activation and endothelial cell inflammation. Am J Physiol Lung Cell Mol Physiol. 2013;305:L651-64. (FC)