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Mouse Anti-Human CD16-UNLB (GRM1)

Cat. No.:
9570-01
Purified Anti-Human CD16 antibody for use in flow cytometry, immunohistochemistry, western blot, immunoprecipitation, ELISA, and CMCD assays.
$108.00
Size Price (USD) Quantity
0.1 mg $108.00
More Information
Clone GRM1
Isotype Mouse (BALB/c) IgG2aκ
Isotype Control Mouse IgG2a-UNLB (HOPC-1)
Specificity Human CD16
Alternative Names FcγRIII, low affinity Fc receptor
Description CD16, a member of the immunoglobulin superfamily, is a 50-65 kDa glycoprotein found as both a transmembrane and GPI-linked form. The transmembrane form of CD16 is expressed on NK cells, granulocytes, macrophages, and mast cells but not on eosinophils. The GPI-anchored type of CD16 is found only on neutrophils. CD16 is involved in NK activation and signal transduction.
Immunogen Mononuclear cells from a prolymphocytic B-leukemia
Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Clonality Monoclonal
Concentration 0.1 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C
Applications Flow Cytometry – Quality tested 6
Immunohistochemistry-Frozen Sections – Reported in literature 3
Immunoprecipitation – Reported in literature 1,2
Western Blot – Reported in literature 3
ELISA – Reported in literature 4,5
Complement Mediated Cell Depletion – Reported in literature 1

RRID Number AB_2796953
Gene ID 2214 (Human)
Gene ID Symbol FCGR3A (Human)
Gene ID Aliases CD16; CD16A; FCG3; FCGR3; FCGRIII; FCR-10; FCRIII; FCRIIIA; IGFR3; IMD20
UniProt ID P08637 (Human)
UniProt Name FCG3A_HUMAN (Human)

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  1. 1. Lopez-Nevot MA, Cabrera T, Huelin C, Ruiz-Cabello F, Garrido F. A study of mAb GRM1 which reacts with natural killer cells and granulocytes. In: McMichael AJ, Beverley PC, Cobbold S, Crumpton MJ, Gilks W, Gotch FM, et al, editors. Leukocyte Typing III: White Cell Differentiation Antigens. Oxford: Oxford University Press; 1987. p. 707. (Immunogen, IP, CMDC)
  2. 2. Uggla CK, Jondal M, Kaplan D, Flomenberg N, Knowles RW. Enhancement of natural killer cell activity by unique antibodies within the CD2 (sheep-RBC receptor) and CD16 (Fc receptor) clusters. In: McMichael AJ, Beverley PC, Cobbold S, Crumpton MJ, Gilks W, Gotch FM, et al, editors. Leukocyte Typing III: White Cell Differentiation Antigens. Oxford: Oxford University Press; 1987. p. 134-7. (IP)
  3. 3. Wainwright SD, Holmes CH. Distribution of Fcγ receptors on trophoblast during human placental development: an immunohistochemical and immunoblotting study. Immunology. 1993;80:343-51. (IHC-FS, WB)
  4. 4. Masuda M, Miyoshi H, Kobatake S, Nishimura N, Dong XH, Komiyama Y, et al. Increased soluble FcγRIIIa in plasma from patients with coronary artery diseases. Artherosclerosis. 2006;188:377-83. (ELISA)
  5. 5. Masuda M, Amano K, Hong SY, Nishimura N, Fukui M, Yoshika M, et al. Soluble FcγRIIIa levels in plasma correlate with carotid maximum intima-media thickness (IMT) in subjects undergoing an annual medical checkup. Mol Med. 2008;14:436-42. (ELISA)
  6. 6. Pilling D, Vakil V, Gomer RH. Improved serum-free culture conditions for the differentiation of human and murine fibrocytes. J Immunol Methods. 2009;351:62-70. (FC)
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