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Mouse Anti-Human IgG4 pFc'-AF488 (HP6023)

Cat. No.:
Alexa Fluor® 488 Anti-Human IgG4 pFc' antibody for use in flow cytometry, immunohistochemistry, and western blot assays.
Size Price (USD) Quantity
0.1 mg $289.00
More Information
Clone HP6023
Isotype Mouse (BALB/c) IgG3κ
Isotype Control 0105-30
Specificity Human IgG4 pFc'
Immunogen Human IgG4 myeloma protein
Conjugate AF488 (Alexa Fluor® 488)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Monoclonal
Concentration 0.5 mg/mL
Volume 0.2 mL
Recommended Storage 2-8°C; Avoid exposure to light
Trademark Information Alexa Fluor® is a registered trademark of Thermo Fisher Scientific, Inc. or its subsidiaries
Applications ELISA – Quality tested 2-9
FLISA – Quality tested
Flow Cytometry – Reported in literature 12-14
Immunohistochemistry-Frozen Sections – Reported in literature 10
Western Blot – Reported in literature 11
Immunoprecipitation – Reported in literature 1
Surface Plasmon Resonance – Reported in literature 15

RRID Number AB_2796687
Gene ID 3503 (Human)
Gene ID Symbol IGHG4 (Human)
UniProt ID P01861 (Human
UniProt Name IGHG4_HUMAN (Human)

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  1. 1. Reimer CB, Phillips DJ, Aloisio CH, Moore DD, Galland GG, Wells TW, et al. Evaluation of thirty-one mouse monoclonal antibodies to human IgG epitopes. Hybridoma. 1984;3:263-75. (Immunogen, IP)
  2. 2. Tomana M, Novak J, Julian BA, Matousovic K, Konecny K, Mestecky J. Circulating immune complexes in IgA nephropathy consist of IgA1 with galactose-deficient hinge region and antiglycan antibodies. J Clin Invest. 1999;104:73-81. (ELISA)
  3. 3. Whary MT, Sundina N, Bravo LE, Correa P, Quinones F, Caro F, et al. Intestinal helminthiasis in Colombian children promotes a Th2 response to Helicobacter pylori: possible implications for gastric carcinogenesis. Cancer Epidemiol Biomarkers Prev. 2005;14:1464-9. (ELISA)
  4. 4. Sabbatini P, Tsuji T, Ferran L, Ritter E, Sedrak C, Tuballes K, et al. Phase I trial of overlapping long peptides from a tumor self-antigen and poly-ICLC shows rapid induction of integrated immune response in ovarian cancer patients. Clin Cancer Res. 2012;18:6497-508. (ELISA)
  5. 5. Itoh M, Weerasooriya MV, Yahathugoda TC, Takagi H, Samarawickrema WA, Nagaoka F, et al. Effects of 5 rounds of mass drug administration with diethylcarbamazine and albendazole on filaria-specific IgG4 titers in urine: 6-year follow-up study in Sri Lanka. Parasitol Int. 2011;60:393-7. (ELISA)
  6. 6. Olaru F, Wang X, Luo W, Ge L, Miner JH, Kleinau S, et al. Proteolysis breaks tolerance toward intact α345(IV) collagen, eliciting novel anti-glomerular basement membrane autoantibodies specific for α345NC1 hexamers. J Immunol. 2013;190:1424-32. (ELISA)
  7. 7. Ferreira AR, Singh B, Cabrera-Mora M, Magri De Souza AC, Queiroz Marques MT, Porto LC, et al. Evaluation of naturally acquired IgG antibodies to a chimeric and non-chimeric recombinant species of Plasmodium vivax reticulocyte binding protein-1: lack of association with HLA-DRB1*/DQB1* in malaria exposed individuals from the Brazilian Amazon. PLoS One. 2014;9(8):e105828. (ELISA)
  8. 8. Haag S, Schneider N, Mason DE, Tuncel J, Andersson IE, Peters EC, et al. Identification of new citrulline-specific autoantibodies, which bind to human arthritic cartilage, by mass spectrometric analysis of citrullinated type II collagen. Arthritis Rheumatol. 2014;66:1440-9. (ELISA)
  9. 9. Lin J, Markowitz GS, Valeri AM, Kambham N, Sherman WH, Appel GB, et al. Renal monoclonal immunoglobulin deposition disease: the disease spectrum. J Am Soc Nephrol. 2001;12:1482-92. (IHC-FS)
  10. 10. de Vos AF, Melief M, van Riel D, Boon L, van Eijk M, de Boer M, et al. Antagonist anti-human CD40 antibody inhibits germinal center formation in cynomolgus monkeys. Eur J Immunol. 2004;34:3446-55. (IHC-FS)
  11. 11. Fischer PU, Curtis KC, Folk SM, Wilkins PP, Marcos LA, Weil GJ. Serological diagnosis of North American Paragonimiasis by Western blot using Paragonimus kellicotti adult worm antigen. Am J Trop Med Hyg. 2013;88:1035-40. (WB)
  12. 12. Liles WC, Dale DC, Price TH, Gaviria JM, Turner T, Saoud J, et al. Inhibition of in vivo neutrophil transmigration by a novel humanized anti-CD11/CD18 monoclonal antibody. Cytokines Cell Mol Ther. 2000;6:121-6. (FC)
  13. 13. Verbon A, Dekkers PE, ten Hove T, Hack CE, Pribble JP, Turner T, et al. IC14, an anti-CD14 antibody, inhibits endotoxin-mediated symptoms and inflammatory responses in humans. J Immunol. 2001;166:3599-605. (FC)
  14. 14. Toyoda C, Suzuki Y, Tsuneyama H, Onodera T, Masuno A, Yabe R, et al. Production of human monoclonal anti-Jk3, recognising an epitope including the Jka/Jkb polymorphic site of the Kidd glycoprotein. Transfus Med. 2014;24:286-91. (FC)
  15. 15. Lewis KB, Hughes RJ, Epstein MS, Josephson NC, Kempton CL, Kessler CM, et al. Phenotypes of allo- and autoimmune antibody responses to FVIII characterized by surface plasmon resonance. PLoS One. 2013;8(5):e61120. (Surface Plasmon Resonance)
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