Ensuring timely antibody delivery in the face of supply chain challenges - contact us for solutions

Mouse Anti-Mouse H-2Dk-BIOT (15-5-5)

Cat. No.:
1914-08
Biotin Anti-Mouse H-2Dk antibody for use in flow cytometry, ELISA, and immunoprecipitation assays.
$303.00
Size Price (USD) Quantity
0.5 mg $303.00
More Information
Clone 15-5-5
Isotype Mouse (C3H.SW) IgG2aκ
Isotype Control Mouse IgG2a-BIOT (HOPC-1)
Specificity Mouse H-2Dk
Alternative Names MHC Class I
Description The monoclonal antibody 15-5-5 reacts with the H-2Dk class I alloantigen. It cross-reacts with H-2Kd and with cells from mice with the H-2f haplotype. The antibody does not react with other (e.g., a, b, p, q, r, s) haplotypes.
Immunogen C3H mouse splenocytes
Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Monoclonal
Concentration 0.5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C
Applications Flow Cytometry – Quality tested 6
Immunoprecipitation – Reported in literature 3
Complement Mediated Cell Depletion – Reported in literature 1,2
ELISA – Reported in literature 4
Blocking – Reported in literature 5

RRID Number AB_2795512
Gene ID 14964 (Mouse)
Gene ID Symbol H2-D1 (Mouse)
Gene ID Aliases H-2D; H2-D; H2-K1
UniProt ID P14426 (Mouse
UniProt Name HA13_MOUSE (Mouse)

Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


Need help with this product?
or call 800.722.2255
  1. 1. Ozato K, Mayer N, Sachs DH. Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and Ia antigens. J Immunol. 1980;124:533-40. (Immunogen, CMDC)
  2. 2. Rogers MJ, Gooding LR, Margulies DH, Evans GA. Analysis of a defect in the H-2 genes of SV40 transformed C3H fibroblasts that do not express H-2Kk. J Immunol. 1983;130:2418-22. (CMDC)
  3. 3. Houlden BA, Widacki SM, Bluestone JA. Signal transduction through class I MHC by a monoclonal antibody that detects multiple murine and human class I molecules. J Immunol. 1991;146:425-30. (IP)
  4. 4. Bedford P, Garner K, Knight SC. MHC class II molecules transferred between allogeneic dendritic cells stimulate primary mixed leukocyte reactions. Int Immunol. 1999;11:1739-44. (ELISA)
  5. 5. Desrosiers M, Kielczewska A, Loredo-Osti J, Adam SG, Makrigiannis AP, Lemieux S, et al. Epistasis between mouse Klra and major histocompatibility complex class I loci is associated with a new mechanism of natural killer cell-mediated innate resistance to cytomegalovirus infection. Nat Genet. 2005;37:593-9. (Block)
  6. 6. Matsuoka S, Asano Y, Sano K, Kishimoto H, Yamashita I, Yorifuji H, et al. A novel type of cell death of lymphocytes induced by a monoclonal antibody without participation of complement. J Exp Med. 1995;181:2007-15. (FC)
See All References