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  • Goat Anti-Human IgG(H+L)-AF647

Goat Anti-Human IgG(H+L)-AF647

Cat. No.:
2015-31
Goat Anti-Human IgG(H+L)-Alexa Fluor® 647 antibody for use in fluorescent assays.
$115.00
Size Price (USD) Quantity
1.0 mg $115.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Goat IgG
Isotype Control Goat IgG-AF647
Specificity Reacts with the heavy and light chains of human IgG and the light chains of human IgM and IgA
Source Pooled antisera from goats hyperimmunized with human IgG
Cross Adsorption None; may react with immunoglobulins from other species
Purification Method Affinity chromatography on human IgG covalently linked to agarose
Conjugate AF647 (Alexa Fluor® 647)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Polyclonal
Concentration 1.0 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C; Avoid exposure to light
Trademark Information Alexa Fluor® is a registered trademark of Thermo Fisher Scientific, Inc. or its subsidiaries
Applications Quality tested applications for relevant formats include -
ELISA
FLISA

RRID Number AB_2795591

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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  1. FLISA plate was coated with Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-UNLB (SB Cat. No. 6300-01). Serially diluted Goat IgG-AF647 (SB Cat. No. 0109-31) was captured and fluorescence intensity quantified.
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  2. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  3. ELISA plate was coated with purified human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Human IgG(H+L), Multi-Species SP ads-BIOT (SB Cat. No. 2087-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat Anti-Human IgG(H+L), Multi-Species SP ads-BIOT
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  4. FLISA plate was coated with purified human IgG, IgM, and IgA. Immunoglobulins were detected with Rabbit Anti-Human IgG(H+L)-FITC (SB Cat. No. 6140-02).
    Rabbit Anti-Human IgG(H+L)-FITC
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  5. Human IgG-UNLB (SB Cat. No. 0150-01) was resolved by electrophoresis, transferred to PVDF membrane, and visualized using Mouse Anti-Human IgG Fc-UNLB (SB Cat. No. 9040-01) followed by Goat Anti-Mouse IgG, Human ads-HRP (SB Cat. No. 1030-05) secondary antibody and chemiluminescent detection.
    Human IgG-UNLB
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  6. FLISA plate was coated with purified human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Human IgG(H+L), Mouse/Bovine/Horse SP ads-AF647 (SB Cat. No. 2016-31).
    Goat Anti-Human IgG(H+L), Mouse/Bovine/Horse SP ads-AF647
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  7. FLISA plate was coated with purified human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Human IgG-AF647 (SB Cat. No. 2040-31).
    Goat Anti-Human IgG-AF647
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  8. ELISA plate was coated with purified human IgG, IgM, and IgA and mouse, bovine, and horse serum. Immunoglobulins and sera were detected with Rabbit Anti-Human IgG(H+L), Mouse/Bovine/Horse SP ads-UNLB (SB Cat. No. 6146-01) followed by Goat Anti-Rabbit IgG(H+L), Mouse/Human ads-HRP (SB Cat. No. 4050-05).
    Rabbit Anti-Human IgG(H+L), Mouse/Bovine/Horse SP ads-UNLB
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  9. FLISA plate was coated with purified human IgM, IgG, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Human IgM-AF647 (SB Cat. No. 2020-31).
    Goat Anti-Human IgM-AF647
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  10. FLISA plate was coated with purified human IgG, IgM, and IgA. Immunoglobulins were detected with Rabbit Anti-Human IgG(H+L), Mouse ads-FITC (SB Cat. No. 6145-02).
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  11. ELISA plate was coated with serially diluted Human IgG<sub>1</sub> Kappa-UNLB (SB Cat. No. 0151K-01). Immunoglobulin was detected with Mouse Anti-Human IgG<sub>1</sub> Hinge-BIOT (SB Cat. No. 9052-08), Mouse Anti-Human IgG<sub>2</sub> Fc-BIOT (SB Cat. No. 9060-08), Mouse Anti-Human IgG<sub>3</sub> Hinge-BIOT (SB Cat. No. 9210-08), Mouse Anti-Human IgG<sub>4</sub> pFc'-BIOT (SB Cat. No. 9190-08), Mouse Anti-Human IgA<sub>1</sub>-BIOT (SB Cat. No. 9130-08), Mouse Anti-Human IgA<sub>2</sub>-BIOT (SB Cat. No. 9140-08), Mouse Anti-Human IgD-BIOT (SB Cat. No. 9030-08), Mouse Anti-Human IgE Fc-BIOT (SB Cat. No. 9160-08), Mouse Anti-Human IgM-BIOT (SB Cat. No. 9020-08), Mouse Anti-Human Kappa-BIOT (SB Cat. No. 9230-08), and Mouse Anti-Human Lambda-BIOT (SB Cat. No. 9180-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05) and quantified.
    Human IgG1 Kappa-UNLB
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  12. ELISA plate was coated with serially diluted Human IgG<sub>1</sub> Lambda-UNLB (SB Cat. No. 0151L-01). Immunoglobulin was detected with Mouse Anti-Human IgG<sub>1</sub> Hinge-BIOT (SB Cat. No. 9052-08), Mouse Anti-Human IgG<sub>2</sub> Fc-BIOT (SB Cat. No. 9060-08), Mouse Anti-Human IgG<sub>3</sub> Hinge-BIOT (SB Cat. No. 9210-08), Mouse Anti-Human IgG<sub>4</sub> pFc'-BIOT (SB Cat. No. 9190-08), Mouse Anti-Human IgA<sub>1</sub>-BIOT (SB Cat. No. 9130-08), Mouse Anti-Human IgA<sub>2</sub>-BIOT (SB Cat. No. 9140-08), Mouse Anti-Human IgD-BIOT (SB Cat. No. 9030-08), Mouse Anti-Human IgE Fc-BIOT (SB Cat. No. 9160-08), Mouse Anti-Human IgM-BIOT (SB Cat. No. 9020-08), Mouse Anti-Human Kappa-BIOT (SB Cat. No. 9230-08), and Mouse Anti-Human Lambda-BIOT (SB Cat. No. 9180-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05) and quantified.
    Human IgG1 Lambda-UNLB
    0151L-01
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  13. Human pancreatic carcinoma cell line MIA PaCa-2 was blocked with 10% Normal Goat Serum (SB Cat. No. 0060-01) and stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat Anti-Mouse IgG(H+L), Human ads-AF555 (SB Cat. No. 1031-32) and DAPI.
    Normal Goat Serum
    0060-01
    $47.00
  14. FLISA plate was coated with purified human IgA, IgG, and IgM. Immunoglobulins were detected with serially diluted Goat Anti-Human IgA-FITC (SB Cat. No. 2050-02).
    Goat Anti-Human IgA-FITC
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    $104.00