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Rat Anti-Human IL-5-BIOT (JES1-5A10)

Cat. No.:
10118-08
Biotin Anti-Human IL-5 antibody for use in flow cytometry, ELISA, and ELISpot assays.
$230.00
Size Price (USD) Quantity
0.5 mg $230.00
More Information
Clone JES1-5A10
Isotype Rat IgG2aκ
Isotype Control Rat IgG2a-BIOT (KLH/G2a-1-1)
Specificity Human/Rhesus/Cynomolgus/Baboon/Sooty Mangabey IL-5
Alternative Names Interleukin-5, eosinophil differentiation factor, EDF, T cell replacing factor-1, TRF-1, eosinophil colony stimulating factor, Eo-CSF, B cell growth factor-2, BCGF-2, B cell differentiation factor for IgM, BCDF-m, IgA enhancing factor, IgA-EF
Immunogen COS-expressed human IL-5
Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Monoclonal
Concentration 0.5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C
Applications ELISA-Capture – Quality tested
ELISA-Detection – Quality tested 1-10
ELISpot-Detection – Reported in literature 4,8,11-14
Flow Cytometry – Reported in literature 6
Neutralization – Reported in literature 1-3

RRID Number AB_2794153
Gene ID 3567 (Human)
710622 (Rhesus)
102125522 (Cynomolgus)
100137220 (Baboon)
105599744 (Sooty Mangabey)
Gene ID Symbol IL5 (Human)
IL5 (Rhesus)
IL5 (Cynomolgus)
IL5 (Baboon)
IL5 (Sooty Mangabey)
Gene ID Aliases EDF; IL-5; TRF
UniProt ID P05113 (Human)
P48093 (Rhesus)
P46685 (Sooty Mangabey)
UniProt Name IL5_HUMAN (Human)
IL5_MACMU (Rhesus)
IL5_CERAT (Sooty Mangabey)

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  1. 1. Abrams JS, Silver JE, Van Dyke RE, Gleich GJ. Eosinophil-active cytokines in human disease: development and use of monoclonal antibodies to IL-3, IL-5 and GM-CSF. In: Gleich GJ, Kay AB, editors. Eosinophils in allergy and inflammation. New York: Marcel Dekker; 1994. p. 133-57. (Immunogen, ELISA-Detection, Neut)
  2. 2. Bacchetta R, de Waal Malefijt R, Yssel H, Abrams J, de Vries JE, Spits H, et al. Host-reactive CD4+ and CD8+ T cell clones isolated from a human chimera produce IL-5, IL-2, IFN-γ and granulocyte/macrophage-colony-stimulating factor but not IL-4. J Immunol. 1990;144:902-8. (ELISA-Detection, Neut)
  3. 3. Abrams JS, Roncarolo M, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992;127:5-24. (ELISA-Detection, Neut)
  4. 4. Mahanty S, Abrams JS, King CL, Limaye AP, Nutman TB. Parallel regulation of IL-4 and IL-5 in human helminth infections. J Immunol. 1992;148:3567-71. (ELISA-Detection, ELISPOT-Detection)
  5. 5. Abrams JS. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001;6.20:1-15. (ELISA-Detection)
  6. 6. Krouwels FH, Nocker RE, Snoek M, Lutter R, van der Zee JS, Weller FR, et al. Immunocytochemical and flow cytofluorimetric detection of intracellular IL-4, IL-5 and IFN-γ: applications using blood- and airway-derived cells. J Immunol Methods. 1997;203:89-101. (ELISA-Detection, FC)
  7. 7. Nyindo M, Kariuki TM, Mola PW, Farah IO, Elson L, Blanton RE, et al. Role of adult worm antigen-specific immunoglobulin E in acquired immunity to Schistosoma mansoni infection in baboons. Infect Immun. 1999;67:636-42. (ELISA-Detection, Baboon Reactivity)
  8. 8. Mäkitalo B, Andersson M, Areström I, Karlén K, Villinger F, Ansari A, et al. ELISpot and ELISA analysis of spontaneous, mitogen-induced and antigen-specific cytokine production in cynomolgus and rhesus macaques. J Immunol Methods. 2002;270:85-97. (ELISA-Detection, ELISPOT-Detection, Rhesus & Cynomolgus Reactivity)
  9. 9. Zhao W, Oskeritzian CA, Pozez AL, Schwartz LB. Cytokine production by skin-derived mast cells: endogenous proteases are responsible for degradation of cytokines. J Immunol. 2005;175:2635-42. (ELISA-Detection)
  10. 10. Janke M, Witsch EJ, Mages HW, Hutloff A, Kroczek RA. Eminent role of ICOS costimulation for T cells interacting with plasmacytoid dendritic cells. Immunology. 2006;118:353-60. (ELISA-Detection)
  11. 11. Guerkov RE, Targoni OS, Kreher CR, Boehm BO, Herrera MT, Tary-Lehmann M, et al. Detection of low-frequency antigen-specific IL-10-producing CD4+ T cells via ELISPOT in PBMC: cognate vs. nonspecific production of the cytokine. J Immunol Methods. 2003;279:111-21. (ELISPOT-Detection)
  12. 12. Wang Z, Metcalf B, Ribeiro RM, McClure H, Kaur A. Th-1-type cytotoxic CD8+ T-lymphocyte responses to simian immunodeficiency virus (SIV) are a consistent feature of natural SIV infection in sooty mangabeys. J Virol. 2006;80:2771-83. (ELISPOT-Detection, Sooty Mangabey Reactivity)
  13. 13. Klinman D. ELISPOT assay to detect cytokine-secreting murine and human cells. Curr Protoc Immunol. 2008;6.19:1-9. (ELISPOT-Detection)
  14. 14. Pichyangkul S, Tongtawe P, Kum-Arb U, Yongvanitchit K, Gettayacamin M, Hollingdale MR, et al. Evaluation of the safety and immunogenicity of Plasmodium falciparum apical membrane antigen 1, merozoite surface protein 1 or RTS,S vaccines with adjuvant system AS02A administered alone or concurrently in rhesus monkeys. Vaccine. 2010;28:452-62. (ELISPOT-Detection, Rhesus Reactivity)
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