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Rat Anti-Mouse CD8α-LE/AF (53-6.7)

Cat. No.:
1550-14
Low Endotoxin/Azide Free Anti-Mouse CD8α antibody for use in flow cytometry, immunohistochemistry / immunocytochemistry, immunoprecipitation, depletion, and blocking assays.
$192.00
Size Price (USD) Quantity
0.5 mg $192.00
More Information
Clone 53-6.7
Isotype Rat (LOU/Ws1/M) IgG2aκ
Isotype Control Rat IgG2a-LE/AF (KLH/G2a-1-1)
Specificity Mouse CD8α
Alternative Names Ly-2, Lyt-2, Ly-35, Ly-B, T8
Description In the mouse, CD8 exists in two forms – (i) a CD8 heterodimer composed of an α chain (CD8α) and a β chain (CD8β); and (ii) a homodimer of two α chains. The heterodimer is found on the surface of essentially all thymocytes and the “suppressor/cytotoxic” subpopulation of mature T lymphocytes. Subsets of intestinal intraepithelial lymphocytes express CD8α without CD8β. It has been suggested that CD8+β- T cells mature extrathymically, while development of the CD8α+β+ population of T cells is thymus-dependent. CD8 acts as a coreceptor with MHC Class I-restricted T cell receptors in antigen recognition and positive selection of MHC class I-restricted CD8+ T cells. In vivo and in vitro treatment with the 53-6.7 monoclonal antibody effectively depletes CD8α+ cells. The 53-6.7 monoclonal antibody also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2.
Immunogen Spleen cells or thymocyte membranes
Conjugate LE/AF (Low Endotoxin/Azide Free)
Buffer Formulation Phosphate buffered saline, pH 7.4
Clonality Monoclonal
Concentration 0.5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C; Handle under aseptic conditions
Applications Flow Cytometry – Quality tested 1,6,7,9-15
Immunohistochemistry-Frozen Sections – Reported in literature 2-5
Immunocytochemistry – Reported in literature 7
Immunoprecipitation – Reported in literature 1,6
Depletion – Reported in literature 8
Blocking – Reported in literature 6

RRID Number AB_2794879
Gene ID 12525 (Mouse)
Gene ID Symbol Cd8a (Mouse)
Gene ID Aliases Ly-2; Ly-B; Ly-35; Lyt-2; BB154331
UniProt ID P01731 (Mouse)
UniProt Name CD8A_MOUSE (Mouse)

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  2. 2. Konno A, Takada K, Saegusa J, Takiguchi M. Presence of B7-2+ dendritic cells and expression of Th1 cytokines in the early development of sialodacryoadenitis in the IQI/Jic mouse model of primary Sjörgren's syndrome. Autoimmunity. 2003;36:247-54. (IHC-FS)
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  8. 8. Hathcock KS. T cell depletion by cytotoxic elimination. Curr Protoc Immunol. 1991;3:4.1. (Depletion)
  9. 9. Lagrota-Candido J, Vasconcellos R, Cavalcanti M, Bozza M, Savino W, Quirico-Santos T. Resolution of skeletal muscle inflammation in mdx dystrophic mouse is accompanied by increased immunoglobulin and interferon-γ production. Int J Exp Path. 2002;83:121-32. (FC)
  10. 10. Parmo-Cabañas M, García-Bernal D, García-Verdugo R, Kremer L, Márquez G, Teixidó J. Intracellular signaling required for CCL25-stimulated T cell adhesion mediated by the integrin α4β1. J Leukoc Biol. 2007;82:380-91. (FC)
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  14. 14. Domingos-Pereira S, Decrausaz L, Derré L, Bobst M, Romero P, Schiller JT, et al. Intravaginal TLR agonists increase local vaccine-specific CD8 T cells and human papillomavirus-associated genital-tumor regression in mice. Mucosal Immunol. 2013;6:393-404. (FC)
  15. 15. Grodeland G, Mjaaland S, Roux KH, Fredriksen AB, Bogen B. DNA vaccine that targets hemagglutinin to MHC class II molecules rapidly induces antibody-mediated protection against influenza. J Immunol. 2013;191:3221-31. (FC)
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