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Rat Anti-Mouse TNF-α-BIOT (MP6-XT22)

Cat. No.:
10230-08
Biotin Anti-Mouse TNF-α antibody for use in flow cytometry, immunohistochemistry / immunocytochemistry, western blot, ELISA, and multiplex assays.
$230.00
Size Price (USD) Quantity
0.5 mg $230.00
More Information
Clone MP6-XT22
Isotype Rat IgG1κ
Isotype Control Rat IgG1-BIOT (KLH/G1-2-2)
Specificity Mouse/Chicken TNF-α
Alternative Names Tumor necrosis factor-α, macrophage cytotoxic factor , MCF, differentiation inducing factor, DIF, cachectin, necrosin, TNFSF-2
Immunogen E. coli-expressed mouse TNF-α
Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Monoclonal
Concentration 0.5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C
Applications ELISA-Capture – Quality tested 2,3
ELISA-Detection – Quality tested 1,4
Flow Cytometry – Quality tested 6-12
ELISpot-Capture – Reported in literature 5
Immunohistochemsitry-Frozen Sections – Reported in literature 13-19
Immunohistochemsitry-Paraffin Sections – Reported in literature 16
Immunocytochemistry – Reported in literature 20,21
Western Blot – Reported in literature 21
Neutralization – Reported in literature 1,22-24
Multiplex-Detection – Reported in literature 25

RRID Number AB_2794275
Gene ID 21926 (Mouse)
Gene ID Symbol Tnf (Mouse)
Gene ID Aliases DIF; Tnfa; TNF-a; TNFSF2; Tnlg1f; Tnfsf1a; TNFalpha; TNF-alpha
UniProt ID P06804 (Mouse)
UniProt Name TNFA_MOUSE (Mouse)

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  1. 1. Abrams JS, Roncarolo M, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992;127:5-24. (ELISA-Detection, Neut)
  2. 2. Heinzel FP, Rerko RM, Ahmed F, Hujer AM. IFN-γ-independent production of IL-12 during murine endotoxemia. J Immunol. 1996;157:4521-8. (ELISA-Capture)
  3. 3. Bordmann G, Rudin W, Favre N. Immunization of mice with phosphatidylcholine drastically reduces the parasitaemia of subsequent Plasmodium chabaudi chabaudi blood-stage infections. Immunology. 1998;94:35-40. (ELISA-Capture)
  4. 4. Abrams JS. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001;6.20:1-15. (ELISA-Detection)
  5. 5. Favre N, Bordmann G, Rudin W. Comparison of cytokine measurements using ELISA, ELISPOT and semi-quantitative RT-PCR. J Immunol Methods. 1997;204:57-66. (ELISPOT-Capture)
  6. 6. Tripp RA, Moore D, Jones L, Sullender W, Winter J, Anderson LJ. Respiratory syncytial virus G and/or SH protein alters Th1 cytokines, natural killer cells, and neutrophils responding to pulmonary infection in BALB/c mice. J Virol. 1999;73:7099-107. (FC)
  7. 7. Asseman C, Mauze S, Leach MW, Coffman RL, Powrie F. An essential role for interleukin 10 in the function of regulatory T cells that inhibit intestinal inflammation. J Exp Med. 1999;190:995-1003. (FC)
  8. 8. Satoh M, Yasunami Y, Matsuoka N, Nakano M, Itoh T, Nitta T, et al. Successful islet transplantation to two recipients from a single donor by targeting proinflammatory cytokines in mice. Transplantation. 2007;83:1085-92. (FC)
  9. 9. Hamada H, Garcia-Hernandez Mde L, Reome JB, Misra SK, Strutt TM, McKinstry KK, et al. Tc17, a unique subset of CD8 T cells that can protect against lethal influenza challenge. J Immunol. 2009;182:3469-81. (FC)
  10. 10. Voß EV, Škuljec J, Gudi V, Skripuletz R, Pul R, Trebst C, et al. Characterisation of microglia during de- and remyelination: can they create a repair promoting environment?. Neurobiol Dis. 2012;45:519-28. (FC)
  11. 11. Le Huu D, Matsushita T, Jin G, Hamaguchi Y, Hasegawa M, Takehara K, et al. Donor-derived regulatory B cells are important for suppression of murine sclerodermatous chronic graft-versus-host disease. Blood. 2013;121:3274-83. (FC)
  12. 12. Nakamura T, Nakao T, Yoshimura N, Ashihara E. Rapamycin prolongs cardiac allograft survival in a mouse model by inducing myeloid-derived suppressor cells. Am J Transplant. 2015 May 5. doi: 10.1111/ajt.13276. [Epub ahead of print]. (FC)
  13. 13. Hunter CA, Litton MJ, Remington JS, Abrams JS. Immunocytochemical detection of cytokines in the lymph nodes and brains of mice resistant or susceptible to toxoplasmic encephalitis. J Infect Dis. 1994;170:939-45. (IHC-FS)
  14. 14. Sunnemark D, Ulfgren A, Örn A, Harris RA. Cytokine production in hearts of Trypanosoma cruzi-infected CBA mice: Do cytokine patterns in chronic stage reflect the establishment of myocardial pathology?. Scand J Immunol. 1996;44:421-9. (IHC-FS)
  15. 15. Marinova-Mutafchieva L, Williams RO, Mason LJ, Mauri C, Feldmann M, Maini RN. Dynamics of proinflammatory cytokine expression in the joints of mice with collagen-induced arthritis (CIA). Clin Exp Immunol. 1997;107:507-12. (IHC-FS)
  16. 16. Whiteland JL, Shimeld C, Nicholls SM, Easty DL, Williams NA, Hill TJ. Immunohistochemical detection of cytokines in paraffin-embedded mouse tissues. J Immunol Methods. 1997;210:103-8. (IHC-FS, IHC-PS)
  17. 17. Issazadeh S, Abdallah K, Chitnis T, Chandraker A, Wells AD, Turka LA, et al. Role of passive T-cell death in chronic experimental autoimmune encephalomyelitis. J Clin Invest. 2000;105:1109-16. (IHC-FS)
  18. 18. Jacobs M, Brown N, Allie N, Gulert R, Ryffel B. Increased resistance to mycobacterial infection in the absence of interleukin-10. Immunology. 2000;100:494-501. (IHC-FS)
  19. 19. Williams RO, Marinova-Mutafchieva L, Feldmann M, Maini RN. Evaluation of TNF-α and IL-1 blockade in collagen-induced arthritis and comparison with combined anti-TNF-α/anti-CD4 therapy. J Immunol. 2000;165:7240-5. (IHC-FS)
  20. 20. Sanders EJ, Prasad S, Hu N, Wride MA. Cell death in the gastrulating chick embryo: potential roles for tumor necrosis factor-alpha (TNF-α). Cell Death Differ. 1997;4:188-99. (ICC, Chicken Reactivity)
  21. 21. Wride MA, Sanders EJ. Expression of tumor necrosis factor-α (TNFα)-cross-reactive proteins during early chick embryo development. Dev Dyn. 1993;198:225-39. (ICC, WB, Chicken Reactivity)
  22. 22. Scanga CA, Mohan VP, Joseph H, Yu K, Chan J, Flynn JL. Reactivation of latent tuberculosis: variations on the Cornell murine model. Infect Immun. 1999;67:4531-8. (Neut)
  23. 23. Via CS, Shustov A, Rus V, Lang T, Nguyen P, Finkelman FD. In vivo neutralization of TNF-α promotes humoral autoimmunity by preventing the induction of CTL. J Immunol. 2001;167:6821-6. (Neut)
  24. 24. Zhou R, Wei H, Sun R, Tian Z. Recognition of double-stranded RNA by TLR3 induces severe small intestinal injury in mice. J Immunol. 2007;178:4548-56. (Neut)
  25. 25. Muir BW, Barden MC, Rylatt DB, Maeji NJ, Hillyard CJ, Gorse AJ, et al, inventors. Bio-Layer Pty Limited, assignee. Use of metal complexes. United States patent US 8,168,445 B2.2012 May 1. (Multiplex-Detection)
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