Ensuring timely antibody delivery in the face of supply chain challenges - contact us for solutions

Rat Anti-Mouse CD40-SPRD (1C10)

Cat. No.:
1645-13
PE/CY5 (SPRD) Anti-Mouse CD40 antibody for use in flow cytometry assays.
$308.00
Size Price (USD) Quantity
0.1 mg $308.00
More Information
Clone 1C10
Isotype Rat (Lewis) IgG2aκ
Isotype Control Rat IgG2a-SPRD (KLH/G2a-1-1)
Specificity Mouse CD40
Alternative Names TNFRSF5, Bp50
Description CD40 is a type I cell surface protein belonging to the tumor necrosis factor superfamily of cell surface receptors. In mice it is expressed on B lineage cells, follicular dendritic cells, thymic epithelium, and interdigitating cells in the T-cell zone of secondary lymphoid organs. CD40 first becomes detectable on a subset of small pre-B II cells in bone marrow with the levels of CD40 expression increasing thereafter during B cell maturation. Immature B cells (IgM+IgDloB220lo) express intermediate levels of CD40, whereas mature B cells (IgM+IgDhiB220hi) express high levels. CD40 has a central role in B cell growth and differentiation, and signaling through CD40 in combination with IL-4 reportedly induces immunoglobulin isotype switching and secretion of IgE. The agonistic 1C10 antibody closely resembles gp39/CD40 ligand in its ability to stimulate proliferation of small, resting B lymphocytes in the absence of other cofactors.
Immunogen sCD40
Conjugate SPRD (R-phycoerythrin/Cyanine 5)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide and a stabilizer
Clonality Monoclonal
Concentration 0.1 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C; Avoid exposure to light; Do not freeze
Trademark Information Spectral Red® is a registered trademark of Southern Biotechnology Associates, Inc.
Spectral Red® is a PE/CY5 tandem conjugate
Cy® is a registered trademark of Global Life Sciences Solutions Germany GmbH
Applications Flow Cytometry – Quality tested 1,7-12
Immunoprecipitation – Reported in literature 1
Activation – Reported in literature 1-4
Blocking – Reported in literature 5,6

RRID Number AB_2795089
Gene ID 21939 (Mouse)
Gene ID Symbol Cd40 (Mouse)
Gene ID Aliases IGM; p50; Bp50; GP39; IMD3; TRAP; HIGM1; T-BAM; Tnfrsf5; AI326936
UniProt ID P27512 (Mouse
UniProt Name TNR5_MOUSE (Mouse)

Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


Need help with this product?
or call 800.722.2255
  1. 1. Heath AW, Wu WW, Howard MC. Monoclonal antibodies to murine CD40 define two distinct functional epitopes. Eur J Immunol. 1994;24:1828-34. (Immunogen, FC, IP, Activ)
  2. 2. Randall TD, Heath AW, Santos-Argumedo L, Howard MC, Weissman IL, Lund FE. Arrest of B lymphocyte terminal differentiation by CD40 signaling: mechanism for lack of antibody-secreting cells in germinal centers. Immunity. 1998;8:733-42. (Activ)
  3. 3. Nojima T, Hayashi K, Goitsuka R, Nakayama K, Nakayama K, Kitamura D. Double knockout mice show BASH and PKCδ have different epistatic relationships in B cell maturation and CD40-mediated activation. Immunol Lett. 2006;105:48-54. (Activ)
  4. 4. Rolph MS, Kaufmann SH. CD40 signaling converts a minimally immunogenic antigen into a potent vaccine against the intracellular pathogen Listeria monocytogenes. J Immunol. 2001;166:5115-21. (Activ)
  5. 5. Kluiver JL, Chen CZ. MicroRNAs regulate B-cell receptor signaling-induced apoptosis. Genes Immun. 2012;13:239-44. (Block)
  6. 6. Baker RL, Wagner DH Jr, Haskins K. CD40 on NOD CD4 T cells contributes to their activation and pathogenicity. J Autoimmun. 2008;31:385-92. (Block)
  7. 7. Brannan CA, Roberts MR. Resident microglia from adult mice are refractory to nitric oxide-inducing stimuli due to impaired NOS2 gene expression. Glia. 2004;48:120-31. (FC)
  8. 8. Zaru R, Mollahan P, Watts C. 3-phosphoinositide-dependent kinase 1 deficiency perturbs toll-like receptor signaling events and actin cytoskeleton dynamics in dendritic cells. J Biol Chem. 2007;283:929-39. (FC)
  9. 9. Brix S, Lund P, Kjaer TM, Straarup EM, Hellgren LI, Frøkiær H. CD4+ T-cell activation is differentially modulated by bacteria-primed dendritic cells, but is generally down-regulated by n-3 polyunsaturated fatty acids. Immunology. 2010;129:338-50. (FC)
  10. 10. Teixeira L, Botelho AS, Mesquita SD, Correia A, Cerca F, Costa R, et al. Plasmacytoid and conventional dendritic cells are early producers of IL-12 in Neospora caninum-infected mice. Immunol Cell Biol. 2010;88:79-86. (FC)
  11. 11. Chen Y, Adams E, Regateiro FS, Vaux DJ, Betz AG, Andersen KG, et al. Activation rather than Foxp3 expression determines that TGF-β-induced regulatory T cells out-compete naïve T cells in dendritic cell clustering. Eur J Immunol. 2012;42:1436-48. (FC)
  12. 12. Jacobsen J, Haabeth OW, Tveita AA, Schjetne KW, Munthe LA, Bogen B. Naive idiotope-specific B and T cells collaborate efficiently in the absence of dendritic cells. J Immunol. 2014;192:4174-83. (FC)
See All References