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Rat Anti-Mouse CD86-APC (2D10)

Cat. No.:
1915-11
APC Anti-Mouse CD86 antibody for use in flow cytometry assays.
$235.00
Size Price (USD) Quantity
0.1 mg $235.00
More Information
Clone 2D10
Isotype Rat (Lewis) IgG2bκ
Isotype Control Rat IgG2b-APC (KLH/G2b-1-2)
Specificity Mouse CD86
Alternative Names B7-2, B70, Ly-58
Description CD86 (B7-2) is an 80 kDa type I integral membrane protein and a member of the immunoglobulin superfamily of cell surface receptors. Along with CD80 (B7-1), CD86 participates in T cell costimulation via interaction with CD28 and CD152 (CTLA-4). It is expressed at very low density in normal tissues but is rapidly upregulated on stimulated B cells, monocytes, and dendritic cells. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response.
Immunogen Mouse B cell lymphoma cell line 5C2
Conjugate APC (Allophycocyanin)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide and a stabilizer
Clonality Monoclonal
Concentration 0.1 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C; Avoid exposure to light; Do not freeze
Applications Flow Cytometry – Quality tested 1,2,4,6,7
Immunohistochemistry-Frozen Sections – Reported in literature 4
Immunoprecipitation – Reported in literature 1
Blocking – Reported in literature 1-3,5
Separation – Reported in literature 2
Complement Mediated Cell Depletion – Reported in literature 2
ELISA – Reported in literature 3

RRID Number AB_2795518
Gene ID 12524 (Mouse)
Gene ID Symbol Cd86 (Mouse)
Gene ID Aliases B7; B7-2; B7.2; B70; CLS1; Cd28l2; ETC-1; Ly-58; Ly58; MB7; MB7-2; TS/A-2
UniProt ID P42082 (Mouse)
UniProt Name CD86_MOUSE (Mouse)

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  1. 1. Chen C, Faherty DA, Gault A, Connaughton SE, Powers GD, Godfrey DI, et al. Monoclonal antibody 2D10 recognizes a novel T cell costimulatory molecule on activated murine B lymphocytes. J Immunol. 1994;152:2105-14. (Immunogen, FC, IP, Block)
  2. 2. Roth R, Nakamura T, Mamula MJ. B7 costimulation and autoantigen specificity enable B cells to activate autoreactive T cells. J Immunol. 1996;157:2924-31. (FC, Sep, CMDC, Block)
  3. 3. Bagenstose LM, Class R, Salgame P, Monestier M. B7-1 and B7-2 co-stimulatory molecules are required for mercury-induced autoimmunity. Clin Exp Immunol. 2002;127:12-19. (Block, ELISA)
  4. 4. Issazadeh S, Navikas V, Schaub M, Sayegh M, Khoury S. Kinetics of expression of costimulatory molecules and their ligands in murine relapsing experimental autoimmune encephalomyelitis in vivo. J Immunol. 1998;161:1104-12. (FC, IHC-FS)
  5. 5. Shanafelt M, Kang I, Barthold SW, Bockenstedt LK. Modulation of murine Lyme borreliosis by interruption of the B7/CD28 T-cell costimulatory pathway. Infect Immun. 1998;66:266-71. (Block)
  6. 6. Grodeland G, Mjaaland S, Roux KH, Fredriksen AB, Bogen B. DNA vaccine that targets hemagglutinin to MHC class II molecules rapidly induces antibody-mediated protection against influenza. J Immunol. 2013;191:3221-31. (FC)
  7. 7. Matsuo H, Yoshimoto N, Iijima M, Niimi T, Jung J, Jeong S, et al. Engineered hepatitis B virus surface antigen L protein particles for in vivo active targeting of splenic dendritic cells. Int J Nanomedicine. 2012;7:3341-50. (FC)
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