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Rat Anti-Mouse CD90.2-PE (30-H12)

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Cat. No.:
PE Anti-Mouse CD90.2 antibody for use in flow cytometry assays.
As low as $110.00
Size Price (USD) Quantity
0.2 mg (Cat. No. 1750-09L) $157.00
0.1 mg (Cat. No. 1750-09) $110.00
More Information
Clone 30-H12
Isotype Rat (LOU/Ws1/M) IgG2bκ
Isotype Control Rat IgG2b-PE (KLH/G2b-1-2)
Specificity Mouse CD90.2
Alternative Names Thy-1.2
Description CD90 (Thy-1) is a GPI-anchored molecule and one of the smallest members of the immunoglobulin superfamily consisting of a single V-set domain. The Thy-1.2 alloantigen is expressed on all thymocytes, peripheral T lymphocytes, and some intraepithelial T cells of most mouse strains. It is also expressed in the brain and at varying levels on other non-lymphoid tissues. The 30-H12 monoclonal antibody does not cross react with mouse and rat strains bearing the Thy-1.1 alloantigen (e.g., AKR/J, PL).
Immunogen Mouse thymus or spleen
Conjugate PE (R-phycoerythrin)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide and a stabilizer
Clonality Monoclonal
Concentration 0.1 mg/mL
Volume 1.0 mL or 2.0 mL
Recommended Storage 2-8°C; Avoid exposure to light; Do not freeze
Applications Flow Cytometry – Quality tested 1,2,9,10
Immunohistochemistry-Frozen Sections – Reported in literature 2,3
Immunocytochemistry – Reported in literature 4
Immunoprecipitation – Reported in literature 1
Depletion – Reported in literature 5,6
Costimulation – Reported in literature 7,8
Apoptosis – Reported in literature 7,8

RRID Number AB_2795234
Gene ID 21838 (Mouse)
Gene ID Symbol Thy1 (Mouse)
Gene ID Aliases T25; CD90; Thy-1; Thy1.1; Thy1.2; Thy-1.2
UniProt ID P01831 (Mouse
UniProt Name THY1_MOUSE (Mouse)

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  1. 1. Ledbetter JA, Herzenberg LA. Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens. Immunol Rev. 1979;47:63-90. (Immunogen, FC, IP)
  2. 2. Ledbetter JA, Rouse RV, Micklem HS, Herzenberg LA. T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views. J Exp Med. 1980;152:280-95. (FC, IHC-FS)
  3. 3. Folgosa L, Zellner HB, El Shikh ME, Conrad DH. Disturbed follicular architecture in B cell A disintegrin and metalloproteinase (ADAM)10 knockouts is mediated by compensatory increases in ADAM17 and TNF-α shedding. J Immunol. 2013;191:5951-8. (IHC-FS)
  4. 4. Sharma N, Suzuki H, He Q, Sharma RP. Tumor necrosis factor α-mediated activation of c-Jun NH2-terminal kinase as a mechanism for fumonisin B1 induced apoptosis in murine primary hepatocytes. J Biochem Mol Toxicol. 2005;19:359-67. (ICC)
  5. 5. Sharma N, He Q, Sharma RP. Amelioration of fumonisin B1 hepatotoxicity in mice by depletion of T cells with anti-Thy-1.2. Toxicology. 2006;223:191-201. (Depletion)
  6. 6. Stevenson MM, Tam MF. Differential induction of helper T cell subsets during blood-stage Plasmodium chabaudi AS infection in resistant and susceptible mice. Clin Exp Immunol. 1993;92:77-83. (Depletion)
  7. 7. Nakashima I, Zhang Y, Rahman SM, Yoshida T, Isobe K, Ding L, et al. Evidence of synergy between Thy-1 and CD3/TCR complex in signal delivery to murine thymocytes for cell death. J Immunol. 1991;147:1153-62. (Costim, Apop)
  8. 8. Nakashima I, Pu M, Hamaguchi M, Iwamoto T, Rahman SM, Zhang Y, et al. Pathway of signal delivery to murine thymocytes triggered by co-crosslinking CD3 and Thy-1 for cellular DNA fragmentation and growth inhibition. J Immunol. 1993;151:3511-20. (Costim, Apop)
  9. 9. Morecki S, Yacovlev E, Gelfand Y, Eizik O, Slavin S. Pretransplant treatment of donors with immunomodulators to control graft-versus-host disease (GVHD) in transplant recipients. Exp Hematol. 2007;35:748-56. (FC)
  10. 10. Chaimowitz NS, Martin RK, Cichy J, Gibb DR, Patil P, Kang D, et al. A disintegrin and metalloproteinase 10 regulates antibody production and maintenance of lymphoid architecture. J Immunol. 2011;187:5114-22. (FC)
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