Ensuring timely antibody supply during the COVID-19 pandemic - contact us for solutions

Rat Anti-Mouse TNF-α-LE/AF (MP6-XT3)

Cat. No:
10229-14
Low Endotoxin/Azide Free Anti-Mouse TNF-α antibody for use in flow cytometry, immunohistochemistry, western blot, ELISA, ELISpot, and neutralization assays.
$280.00
Size Price (USD) Quantity
0.5 mg $280.00
More Information
Clone MP6-XT3
Isotype Rat IgG1κ
Isotype Control Rat IgG1-LE/AF (KLH/G1-2-2)
Specificity Mouse TNF-α
Alternative Names Tumor necrosis factor-α, macrophage cytotoxic factor , MCF, differentiation inducing factor, DIF, cachectin, necrosin, TNFSF-2
Immunogen E. coli-expressed mouse TNF-α
Conjugate LE/AF (Low Endotoxin/Azide Free)
Buffer Formulation Phosphate buffered saline, pH 7.4
Clonality Monoclonal
Concentration 0.5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C; Handle under aseptic conditions
Applications ELISA-Detection – Quality tested 2
ELISA-Capture – Reported in literature 1,3
ELISpot-Capture – Reported in literature 4
ELISpot-Detection – Reported in literature 5
Flow Cytometry – Reported in literature 5-7
Immunohistochemsitry-Frozen Sections – Reported in literature 8-13
Immunohistochemsitry-Paraffin Sections – Reported in literature 14
Western Blot – Reported in literature 15
Neutralization – Reported in literature 1,16,17
Multiplex-Detection – Reported in literature 2

RRID Number AB_2794272
Gene ID 21926 (Mouse)
Gene ID Symbol Tnf (Mouse)
Gene ID Aliases DIF; Tnfa; TNF-a; TNFSF2; Tnlg1f; Tnfsf1a; TNFalpha; TNF-alpha
UniProt ID P06804 (Mouse)
UniProt Name TNFA_MOUSE (Mouse)

Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


Need help with this product?
or call 800.722.2255
  1. 1. Abrams JS, Roncarolo M, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992;127:5-24. (ELISA-Capture, Neut)
  2. 2. Carson RT, Vignali DA. Simultaneous quantitation of 15 cytokines using a multiplexed flow cytometric assay. J Immunol Methods. 1999;227:41-52. (ELISA-Detection, Multiplex-Detection)
  3. 3. Abrams JS. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001;6.20:1-15. (ELISA-Capture)
  4. 4. Bordmann G, Rudin W, Favre N. Immunization of mice with phosphatidylcholine drastically reduces the parasitaemia of subsequent Plasmodium chabaudi chabaudi blood-stage infections. Immunology. 1998;94:35-40.(ELISPOT-Capture)
  5. 5. Korten S, Anderson RJ, Hannan CM, Sheu EG, Sinden R, Gadola S, et al. Invariant Vα14 chain NKT cells promote Plasmodium berghei circumsporozoite protein-specific gamma interferon- and tumor necrosis factor alpha-producing CD8+ T cells in the liver after poxvirus vaccination of mice. Infect Immun. 2005;73:849-58. (ELISPOT-Detection, FC)
  6. 6. Zajac AJ, Quinn DG, Cohen PL, Frelinger JA. Fas-dependent CD4+ cytotoxic T-cell-mediated pathogenesis during virus infection. Proc Natl Acad Sci USA. 1996;93:14730-5. (FC)
  7. 7. Feng H, Zhang D, Palliser D, Zhu P, Cai S, Schlesinger A, et al. Listeria-infected myeloid dendritic cells produce IFN-β, priming T cell activation. J Immunol. 2005;175:421-32. (FC)
  8. 8. Henke A, Huber S, Stelzner A, Whitton JL. The role of CD8+ T lymphocytes in coxsackievirus B3-induced myocarditis. J Virol. 1995;69:6720-8. (IHC-FS)
  9. 9. Martinelli TM, Van Driel IR, Alderuccio F, Gleeson PA, Toh B. Analysis of mononuclear cell infiltrate and cytokine production in murine autoimmune gastritis. Gastroenterology. 1996;110:1791-802. (IHC-FS)
  10. 10. Morris MM, Dyson H, Baker D, Harbige LS, Fazakerley JK, Amor S. Characterization of the cellular and cytokine response in the central nervous system following Semliki Forest virus infection. J Neuroimmunol. 1997;74:185-97. (IHC-FS)
  11. 11. Hersmann GH, Kriegsmann J, Simon J, Hüttich C, Bräuer R. Expression of cell adhesion molecules and cytokines in murine antigen-induced arthritis. Cell Adhes Commun. 1998;6:69-82. (IHC-FS)
  12. 12. Tsao N, Kanakamma PP, Luh T, Chou C, Lei H. Inhibition of Escherichia coli-induced meningitis by carboxyfullerence. Antimicrob Agents Chemother. 1999;43:2273-7. (IHC-FS)
  13. 13. Tsao N, Hsu HP, Wu CM, Liu CC, Lei HY. Tumour necrosis factor-α causes an increase in blood-brain barrier permeability during sepsis. J Med Microbiol. 2001;50:812-21. (IHC-FS)
  14. 14. Huang T, Tsai S, Liu L, Liu YL, Liu H, Chuang KP. Effect of Arctium lappa L. in the dextran sulfate sodium colitis mouse model. World J Gastroenterol. 2010;16:4193-9. (IHC-PS)
  15. 15. Rogove AD, Lu W, Tsirka SE. Microglial activation and recruitment, but not proliferation, suffice to mediate neurodegeneration. Cell Death Differ. 2002;9:801-6. (WB)
  16. 16. Grohmann U, Van Snick J, Campanile F, Silla S, Giampietri A, Vacca C, et al. IL-9 protects mice from Gram-negative bacterial shock: suppression of TNF-α, IL-12, and IFN-γ, and induction of IL-10. J Immunol. 2000;164:4197-203. (Neut)
  17. 17. Ghorpade DS, Holla S, Kaveri SV, Bayry J, Patil SA, Balaji KN. Sonic hedgehog-dependent induction of microRNA 31 and microRNA 150 regulates Mycobacterium bovis BCG-driven toll-like receptor 2 signaling. Mol Cell Biol. 2013;33:543-56. (Neut)
See All References