Mouse Anti-Cytokeratin 19-HRP

Cat. No.
10090-05  
Size
1.0 mL  
Price (USD)
$300.00 
Clone SB39g
Isotype Mouse (BALB/c) IgG2aκ
Isotype Control Mouse IgG2a-HRP
Immunogen Recombinant C-terminal cytokeratin 19
Specificity Cytokeratin 19
Alternalte Name(s) CK 19, keratin 19
Description Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1-8). The two keratin types share only 30% sequence homology. Keratins demonstrate tissue- and differentiation-specific expression profiles. Cytokeratin 19 is the smallest human keratin. It is found in many simple and some non-keratinizing stratified squamous epithelia and is often co-expressed with keratin 7. Cytokeratin 19 has been implicated as a marker for stem cells in some tissues such as hair follicles in the skin. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification. The degree of cytokeratin 19 positivity in breast cancer distinguishes malignant from benign tumors. Cytokeratin 19 is also associated with malignant transformation in esophageal and pancreatic cancers.
Format/Conjugate HRP (Horseradish Peroxidase)
Buffer Formulation Supplied in a stock solution of 50% glycerol/50% PBS, pH 7.4; No preservative added
Volume 1.0 mL
Storage & Handling Store at 2-8°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
ELISA – Quality tested
FLISA – Quality tested
Western Blot – Quality tested
Immunohistochemistry-Paraffin Sections 2
Immunocytochemistry 2
Flow Cytometry – Reported in literature 1
Immunoprecipitation 3
Recommended Dilutions Please refer to product specific Technical Bulletin
  • Paraffin embedded human gastric cancer tissue was stained with Mouse IgG2a-HRP isotype control (SB Cat. No. 0103-05; left) and Mouse Anti-Cytokeratin 19-HRP (SB Cat. No. 10090-05; right) followed by DAB and hematoxylin.

Paraffin embedded human gastric cancer tissue was stained with Mouse IgG2a-HRP isotype control (SB Cat. No. 0103-05; left) and Mouse Anti-Cytokeratin 19-HRP (SB Cat. No. 10090-05; right) followed by DAB and hematoxylin.
 
References
1. Berglin L, Björkström NK, Bergquist A. Primary sclerosing cholangitis is associated with autoreactive IgA antibodies against biliary epithelial cells. Scand J Gastroenterol. 2013;48:719-28. (FC)
2. SouthernBiotech published data (IHC-PS, ICC)
3. SouthernBiotech unpublished data (IP)