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Rat Anti-Human IL-10-BIOT

Cat. No.
10110-08  
Size
0.5 mg  
Price (USD)
$230.00 
Qty
Add to Cart
Clone JES3-12G8
Isotype Rat IgG2aκ
Isotype Control Mouse IgG2a-BIOT
Immunogen COS-expressed human IL-10
Specificity Human/Viral/Cynomolgus IL-10
Alternalte Name(s) Interleukin-10, cytokine synthesis inhibitory factor, CSIF, TGIF, B-TCGF
Format/Conjugate BIOT (Biotin)
Buffer Formulation Supplied in PBS/NaN3
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling Store at 2-8°C
Please refer to product specific (M)SDS
Applications
Applications for relevant formats of this clone include -
ELISA-Detection – Quality tested 1-4,6
ELISpot-Detection – Reported in literature 5-7
Immunohistochemistry-Frozen Sections – Reported in literature 8-10
Immunohistochemistry-Paraffin Sections – Reported in literature 11
Immunoblotting – Reported in literature 4,12
Neutralization – Reported in literature 1,12,13
Multiplex-Detection – Reported in literature 14

Note – May be paired with the purified clone JES3-9D7 (SB Cat. No. 10100-01) in a sandwich ELISA
Recommended Dilutions Please refer to product specific Technical Bulletin
  • Standard curve generated with Rat Anti-Human IL-10-UNLB (SB Cat. No. 10100-01; Clone JES3-9D7) and Rat Anti-Human IL-10-BIOT (SB Cat. No. 10110-08; Clone JES3-12G8) followed by Streptavidin-HRP (SB Cat. No. 7100-05)

Standard curve generated with Rat Anti-Human IL-10-UNLB (SB Cat. No. 10100-01; Clone JES3-9D7) and Rat Anti-Human IL-10-BIOT (SB Cat. No. 10110-08; Clone JES3-12G8) followed by Streptavidin-HRP (SB Cat. No. 7100-05)
 
References
1. Abrams JS, Roncarolo M, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992;127:5-24. (ELISA-Detection, Neut)
2. Gotlieb WH, Abrams JS, Watson JM, Velu TJ, Berek JS, Martínez-Maza O. Presence of interleukin 10 (IL-10) in the ascites of patients with ovarian and other intra-abdominal cancers. Cytokine. 1992;4:385-90. (ELISA-Detection, Viral Reactivity)
3. Abrams JS. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001;6.20:1-15. (ELISA-Detection)
4. Kawamoto S, Nitta Y, Tashiro F, Nakano A, Yamato E, Tahara H, et al. Suppression of Th1 cell activation and prevention of autoimmune diabetes in NOD mice by local expression of viral IL-10. Int Immunol. 2001;13:685-94. (ELISA-Detection, WB, Viral Reactivity)
5. Hansson T, Dannæus A, Klareskog L. Cytokine-producing cells in peripheral blood of children with coeliac disease secrete cytokines with a type 1 profile. Clin Exp Immunol. 1999;116:246-50. (ELISPOT-Detection)
6. Guerkov RE, Targoni OS, Kreher CR, Boehm BO, Herrera MT, Tary-Lehmann M, et al. Detection of low-frequency antigen-specific IL-10-producing CD4+ T cells via ELISPOT in PBMC: cognate vs. nonspecific production of the cytokine. J Immunol Methods. 2003;279:111-21. (ELISA-Detection, ELISPOT-Detection)
7. Klinman D. ELISPOT assay to detect cytokine-secreting murine and human cells. Curr Protoc Immunol. 2008;6.19:1-9. (ELISPOT-Detection)
8. Andersson J, Abrams J, Björk L, Funa K, Litton M, Ågren K, et al. Concomitant in vivo production of 19 different cytokines in human tonsils. Immunology. 1994;83:16-24. (IHC-FS)
9. Ulfgren A, Lindblad S, Klareskog L, Andersson J, Andersson U. Detection of cytokine producing cells in the synovial membrane from patients with rheumatoid arthritis. Ann Rheum Dis. 1995;54:654-61. (IHC-FS)
10. Bergeron A, Soler P, Kambouchner M, Loiseau P, Milleron B, Valeyre D, et al. Cytokine profiles in idiopathic pulmonary fibrosis suggest an important role for TGF-β and IL-10. Eur Respir J. 2003;22:69-76. (IHC-FS)
11. Bellone G, Carbone A, Smirne C, Scirelli T, Buffolino A, Novarino A, et al. Cooperative induction of a tolerogenic dendritic cell phenotype by cytokines secreted by pancreatic carcinoma cells. J Immunol. 2006;177:3448-60. (IHC-PS)
12. Brodeur ND, Spencer JV. Antibodies to human IL-10 neutralize ebvIL-10-mediated cytokine suppression but have no effect on cmvIL-10 activity. Virus Res. 2010;153:265-8. (WB, Neut, Viral Reactivity)
13. Groux H, O'Garra A, Bigler M, Rouleau M, Antonenko S, de Vries JE, et al. A CD4+ T-cell subset inhibits antigen-specific T-cell responses and prevents colitis. Nature. 1997;389:737-42. (Neut)
14. Hutchinson KL, Villinger F, Miranda ME, Ksiazek TG, Peters CJ, Rollin PE. Multiplex analysis of cytokines in the blood of cynomolgus macaques naturally infected with Ebola virus (Reston serotype). J Med Virol. 2001;65:561-6. (Multiplex-Detection, Cynomolgus Reactivity)
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