Rat Anti-Mouse IL-3-BIOT

Cat. No.
10213-08  
Size
0.5 mg   		Price (USD)
$275.00 
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Technical Bulletin Certificate of
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Clone MP2-43D11
Isotype Rat IgG2aκ
Isotype Control Rat IgG2a-BIOT
Immunogen COS-expressed mouse IL-3
Specificity Mouse IL-3
Alternalte Name(s) Interleukin-3, burst promoting activity, BP/BPA, eosinophil colony stimulating factor, Eo-CSF, hematopoietic cell growth factor, HCGF, mast cell growth factor, MGF, MCGF, multi-colony stimulating factor, multi-CSF, P cell stimulating activity, PCSA
Format/Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
ELISA-Detection – Quality tested 2-5
ELISpot-Detection – Reported in literature 6,7
Immunohistochemsitry-Frozen Sections – Reported in literature 8,9
Immunocytochemistry – Reported in literature 3
Western Blot – Reported in literature 1
Neutralization – Reported in literature 1,3
Multiplex-Detection – Reported in literature 4

Note – May be paired with the purified clone MP2-8F8 (SB Cat. No. 10212-01) in a sandwich ELISA
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2794253
Gene ID
16187 (Mouse)
Gene Symbol
Il3 (Mouse)
Gene Aliases BPA; PSF; HCGF; Il-3; MCGF; Csfmu
Uniprot ID
P01586 (Mouse)
Uniprot Name
IL3_MOUSE 
  • Rat Anti-Mouse IL-3-BIOT
    Standard curve generated with Rat Anti-Mouse IL-3-UNLB (SB Cat. No. 10212-01; Clone MP2-8F8) and Rat Anti-Mouse IL-3-BIOT (SB Cat. No. 10213-08; Clone MP2-43D11) followed by Streptavidin-HRP (SB Cat. No. 7100-05)

Standard curve generated with Rat Anti-Mouse IL-3-UNLB (SB Cat. No. 10212-01; Clone MP2-8F8) and Rat Anti-Mouse IL-3-BIOT (SB Cat. No. 10213-08; Clone MP2-43D11) followed by Streptavidin-HRP (SB Cat. No. 7100-05)
 
Related Products (5)
References
1. Abrams JS, Pearce MK. Development of rat anti-mouse interleukin 3 monoclonal antibodies which neutralize bioactivity in vitro. J Immunol. 1988;140:131-7. (Immunogen, WB, Neut)
2. Abrams JS, Roncarolo M, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992;127:5-24. (ELISA-Detection)
3. Sander B, Höidén I, Andersson U, Möller E, Abrams JS. Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining. J Immunol Methods. 1993;166:201-14. (ELISA-Detection, ICC, Neut)
4. Carson RT, Vignali DA. Simultaneous quantitation of 15 cytokines using a multiplexed flow cytometric assay. J Immunol Methods. 1999;227:41-52. (ELISA-Detection, Multiplex-Detection)
5. Abrams JS. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001;6.20:1-15. (ELISA-Detection)
6. Karulin AY, Hesse MD, Tary-Lehmann M, Lehmann PV. Single-cytokine-producing CD4 memory cells predominate in type 1 and type 2 immunity. J Immunol. 2000;164:1862-72. (ELISPOT-Detection)
7. Klinman D. ELISPOT assay to detect cytokine-secreting murine and human cells. Curr Protoc Immunol. 2008;6.19:1-9. (ELISPOT-Detection)
8. Martinelli TM, Van Driel IR, Alderuccio F, Gleeson PA, Toh B. Analysis of mononuclear cell infiltrate and cytokine production in murine autoimmune gastritis. Gastroenterology. 1996;110:1791-802. (IHC-FS)
9. Morris MM, Dyson H, Baker D, Harbige LS, Fazakerley JK, Amor S. Characterization of the cellular and cytokine response in the central nervous system following Semliki Forest virus infection. J Neuroimmunol. 1997;74:185-97. (IHC-FS)