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Goat Anti-Mouse IgG2a-BIOT

Cat. No.
1081-08  
Size
1.0 mg  
Price (USD)
$161.00 
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Specificity Reacts with the heavy chain of mouse IgG2a
Source Pooled antisera from goats hyperimmunized with mouse IgG2a
Cross Adsorption Mouse IgG1, IgG2b, IgG3, IgM, and IgA; may react with immunoglobulins from other species
Purification Affinity chromatography on mouse IgG2a covalently linked to agarose
Isotype Goat IgG
Isotype Control Goat IgG-BIOT
Format/Conjugate BIOT (Biotin)
Buffer Formulation Supplied in PBS/NaN3
Concentration 0.5 mg/mL
Volume 2.0 mL
Storage & Handling Store at 2-8°C
Please refer to product specific SDS
Applications
Quality tested applications for relevant formats include -
ELISA
FLISA
Other referenced applications for relevant formats include -
Immunohistochemistry-Frozen Sections 1
Immunocytochemistry 1
Western Blot 2
Recommended Dilutions Please refer to product specific Technical Bulletin
  • ELISA plate was coated with purified mouse IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG2a-BIOT (SB Cat. No. 1081-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).

ELISA plate was coated with purified mouse IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG2a-BIOT (SB Cat. No. 1081-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
 
References
1. Piersma B, de Rond S, Werker PM, Boo S, Hinz B, van Beuge MM, et al. YAP1 is a driver of myofibroblast differentiation in normal and diseased fibroblasts. Am J Pathol. 2015;185:3326-37. (IHC-FS, ICC)
2. Bruns SA. Autophagy regulation by the p38 MAP kinase [dissertation]. Magdeburg (Germany): Otto-von-Guericke-Universität Magdeburg; 2017. (WB)
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