Cat. No.
1175-08

Size
0.5 mg

Price (USD)
$185.00

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Technical Bulletin Certificate of
Analysis Safety Data Sheet Bulk/Custom
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Clone	JC5-1
Isotype	Rat IgG_2bκ
Isotype Control	Rat IgG_2b-BIOT
Specificity	Mouse Lambda
Format/Conjugate	BIOT (Biotin)
Buffer Formulation	Phosphate buffered saline containing < 0.1% sodium azide
Concentration	0.5 mg/mL
Volume	1.0 mL
Storage & Handling	2-8°C Please refer to product specific SDS
Applications	Applications for relevant formats of this clone include - Flow Cytometry – Quality tested ^1-14 ELISA – Quality tested FLISA – Quality tested Immunohistochemistry-Frozen Sections – Reported in literature ^15,16 Western Blot-Non-Reducing ¹⁸ Separation – Reported in literature ¹⁷
Recommended Dilutions	Please refer to product specific Technical Bulletin
RRID	AB_2794674

Gene ID	111519 (Mouse)
Gene Symbol	Igl (Mouse)
Gene Aliases	Igl-x

ELISA plate was coated with purified mouse IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Rat Anti-Mouse Lambda-BIOT (SB Cat. No. 1175-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
BALB/c mouse splenocytes were stained with Rat Anti-Mouse Lambda-BIOT (SB Cat. No. 1175-08) and Rat Anti-Mouse CD19-PE (SB Cat. No. 1575-09) followed by Streptavidin-FITC (SB Cat. No. 7100-02).

ELISA plate was coated with purified mouse IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Rat Anti-Mouse Lambda-BIOT (SB Cat. No. 1175-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).

BALB/c mouse splenocytes were stained with Rat Anti-Mouse Lambda-BIOT (SB Cat. No. 1175-08) and Rat Anti-Mouse CD19-PE (SB Cat. No. 1575-09) followed by Streptavidin-FITC (SB Cat. No. 7100-02).

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References

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3.	O'Neill SK, Shlomchik MJ, Glant TT, Cao Y, Doodes PD, Finnegan A. Antigen-specific B cells are required as APCs and autoantibody-producing cells for induction of severe autoimmune arthritis. J Immunol. 2005;174:3781-8. (FC)
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9.	Rayamajhi M, Delgado C, Condon TV, Riches DW, Lenz LL. Lung B cells promote early pathogen dissemination and hasten death from inhalation anthrax. Mucosal Immunol. 2012;5:444-54. (FC)
10.	Andrews SF, Zhang Q, Lim S, Li L, Lee J, Zheng N, et al. Global analysis of B cell selection using an immunoglobulin light chain-mediated model of autoreactivity. J Exp Med. 2013;210:125-42. (FC)
11.	Peng C, Eckhardt LA. Role of the Igh intronic enhancer Eμ in clonal selection at the pre-B to immature B cell transition. J Immunol. 2013;191:4399-411. (FC)
12.	Nechanitzky R, Akbas D, Scherer S, Györy I, Hoyler T, Ramamoorthy S, et al. Transcription factor EBF1 is essential for the maintenance of B cell identity and prevention of alternative fates in committed cells. Nat Immunol. 2013;14:867-75. (FC)
13.	Lee E, Liang Q, Ali H, Bayliss L, Beasley A, Bloomfield-Gerdes T, et al. Complete humanization of the mouse immunoglobulin loci enables efficient therapeutic antibody discovery. Nat Biotechnol. 2014;32:356-63. (FC)
14.	Hu J, Oda SK, Shotts K, Donovan EE, Strauch P, Pujanauski LM, et al. Lysophosphatidic acid receptor 5 inhibits B cell antigen receptor signaling and antibody response. J Immunol. 2014;193:85-95. (FC)
15.	Källberg E, Leanderson T. A subset of dendritic cells express joining chain (J-chain) protein. Immunology. 2008;123:590-9. (IHC-FS)
16.	Swanson CL, Wilson TJ, Strauch P, Colonna M, Pelanda R, Torres RM. Type I IFN enhances follicular B cell contribution to the T cell-independent antibody response. J Exp Med. 2010;207:1485-1500. (IHC-FS)
17.	Xiang Y, Garrard WT. The downstream transcriptional enhancer, Ed, positively regulates mouse Ig κ gene expression and somatic hypermutation. J Immunol. 2008;180:6725-32. (Sep)
18.	SouthernBiotech unpublished data (WB-NR)

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