Rat Anti-Mouse CD5-SPRD

Cat. No.
1545-13  
Size
0.1 mg  
Price (USD)
$225.00 
Clone B19.1
Isotype Rat IgG2aκ
Isotype Control Rat IgG2a-SPRD
Specificity Mouse CD5
Alternalte Name(s) Lyt-1, Ly-1, Lymphocyte antigen 1, Ly-12, Ly-A, T1, Tp67
Description CD5, also known as Lyt-1, is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T lymphocytes, and a subset of B lymphocytes, but not on natural killer (NK) cells. It has been identified as the major ligand of the B-cell antigen CD72. The frequency of CD5+ B cells exhibits strain-dependent variation, and the phenotypic, anatomical, functional, developmental, and pathological characteristics of the CD5+ B cells suggest that they may represent a distinct lineage, known as B-1 cells. Binding of CD5 on the T cell surface can augment alloantigen- or mitogen-induced lymphocyte proliferation and induces increased cytosolic free calcium, IL-2 secretion, and IL-2R expression. It has been proposed that CD5 negatively regulates signal transduction mediated by the T-cell and B-cell receptors.
Format/Conjugate SPRD (R-phycoerythrin/Cyanine 5)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide and a stabilizer
Concentration 0.1 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C; Avoid exposure to light; Do not freeze
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 1-4
Immunoprecipitation – Reported in literature 5
Western Blot – Reported in literature 5
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2794854
Gene ID
12507 (Mouse)
Gene Symbol
Cd5 (Mouse)
Gene Aliases Ly-1; Ly-12; Ly-A; Lyt-1
Uniprot ID
P13379 (Mouse)
Uniprot Name
  • C57BL/6 mouse splenocytes were stained with Rat Anti-Mouse CD5-UNLB (SB Cat. No. 1545-01) followed by Mouse Anti-Rat IgG2a-FITC (SB Cat. No. 3065-02).

C57BL/6 mouse splenocytes were stained with Rat Anti-Mouse CD5-UNLB (SB Cat. No. 1545-01) followed by Mouse Anti-Rat IgG2a-FITC (SB Cat. No. 3065-02).
 
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References
1. Seagal J, Edry E, Keren Z, Leider N, Benny O, Machluf M, et al. A fail-safe mechanism for negative selection of isotype-switched B cell precursors is regulated by the Fas/FasL pathway. J Exp Med. 2003;198:1609-19. (FC)
2. Berge T, Grønningsæter IH, Lorvik KB, Abrahamsen G, Granum S, Sundvold-Gjerstad V, et al. SH2D2A modulates T cell mediated protection to a B cell derived tumor in transgenic mice. PLoS One. 2012;7(10):e48239. (FC)
3. Zhu Z, Li R, Li H, Zhou T, Davis RS. FCRL5 exerts binary and compartment-specific influence on innate-like B-cell receptor signaling. Proc Natl Acad Sci USA. 2013;110:E1282-90. (FC)
4. Almena M, Andrada E, Liebana R, Merida I. Diacylglycerol metabolism attenuates T-cell receptor signaling and alters thymocyte differentiation. Cell Death Dis. 2013;4:e912. (FC)
5. Personal communication (IP, WB)
Spectral Red® is a registered trademark of Southern Biotechnology Associates, Inc.
Spectral Red® is a PE/CY5 tandem conjugate
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