Rat Anti-Mouse CD11b-FITC

Cat. No.
1560-02  
Size
0.5 mg   		Price (USD)
$220.00 
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Clone 3A33
Isotype Rat (Lewis) IgG2aκ
Isotype Control Rat IgG2a-FITC
Immunogen Peritoneal macrophages from B6D2 hybrid mice
Specificity Mouse CD11b
Alternalte Name(s) Integrin αM, Mac-1α, Mac-1, Mo1, CR3, C3biR
Description CD11b (integrin αM subunit) combines with CD18 (integrin β2 subunit) to form the integrin Mac-1, also known as complement receptor 3 (CR3), which mediates adhesion to C3bi and ICAM-1 (CD54). Mac-1 is expressed at varying levels on granulocytes, macrophages, dendritic cells, NK cells, and B-1 cells in the peritoneal and pleural cavities. In addition to its role in binding C3bi on opsonized targets and mediation of the subsequent ingestion process, Mac-1 is important as an adhesion molecule in the transendothelial migration of monocytes and neutrophils.
Format/Conjugate FITC (Fluorescein)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C; Avoid exposure to light
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 1,4-13
Immunohistochemistry-Frozen Sections – Reported in literature 14
Immunocytochemistry – Reported in literature 2
Immunoprecipitation – Reported in literature 1
Blocking – Reported in literature 3
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2794897
Gene ID
16409 (Mouse)
Gene Symbol
Itgam (Mouse)
Gene Aliases CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik
Uniprot ID
P05555 (Mouse)
Uniprot Name
ITAM_MOUSE 
  • Rat Anti-Mouse CD11b-FITC
    BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD11b-FITC (SB Cat. No. 1560-02) and Rat Anti-Mouse Ly-6G/Ly-6C-PE (SB Cat. No. 1900-09).
  • Rat Anti-Mouse CD11b-FITC
    Frozen thermally burned mouse tissue section was stained with Rat Anti-Mouse CD11b-FITC (SB Cat. No. 1560-02) and Rat Anti-Mouse Ly-6G/Ly-6C-PE (SB Cat. No. 1900-09) followed by mounting with DAPI containing medium.

    Image from Everett J, Turner K, Cai Q, Gordon V, Whiteley M, Rumbaugh K. Arginine is a critical substrate for the pathogenesis of Pseudomonas aeruginosa in burn wound infections. MBio. 2017;8:e02160-16. Figure S1
  • Rat Anti-Mouse CD11b-FITC
    Frozen thermally burned mouse tissue section was stained with Rat Anti-Mouse CD11b-FITC (SB Cat. No. 1560-02) and mounted with DAPI containing medium.

    Image from Everett J, Turner K, Cai Q, Gordon V, Whiteley M, Rumbaugh K. Arginine is a critical substrate for the pathogenesis of Pseudomonas aeruginosa in burn wound infections. MBio. 2017;8:e02160-16. Figure 1(c)

BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD11b-FITC (SB Cat. No. 1560-02) and Rat Anti-Mouse Ly-6G/Ly-6C-PE (SB Cat. No. 1900-09).
 

Frozen thermally burned mouse tissue section was stained with Rat Anti-Mouse CD11b-FITC (SB Cat. No. 1560-02) and Rat Anti-Mouse Ly-6G/Ly-6C-PE (SB Cat. No. 1900-09) followed by mounting with DAPI containing medium.

Image from Everett J, Turner K, Cai Q, Gordon V, Whiteley M, Rumbaugh K. Arginine is a critical substrate for the pathogenesis of Pseudomonas aeruginosa in burn wound infections. MBio. 2017;8:e02160-16. Figure S1
 

Frozen thermally burned mouse tissue section was stained with Rat Anti-Mouse CD11b-FITC (SB Cat. No. 1560-02) and mounted with DAPI containing medium.

Image from Everett J, Turner K, Cai Q, Gordon V, Whiteley M, Rumbaugh K. Arginine is a critical substrate for the pathogenesis of Pseudomonas aeruginosa in burn wound infections. MBio. 2017;8:e02160-16. Figure 1(c)
 
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References
1. Martin A, Le Corre R, Pellen P, Bourel D, Merdrignac G, Genetet B, et al. A monoclonal antibody (3A33) that reacts with a mouse-specific epitope of Mac-1 antigen. Tissue Antigens. 1986;28:15-23. (Immunogen, IP, FC)
2. Wang M, Shakhatreh MK, James D, Liang S, Nishiyama S, Yoshimura F, et al. Fimbrial proteins of porphyromonas gingivalis mediate in vivo virulence and exploit TLR2 and complement receptor 3 to persist in macrophages. J Immunol. 2007;179:2349-58. (ICC)
3. Collet B, Martin A, Merdrignac G, Genetet B, Toujas L. Modulation of expression of mouse macrophage surface antigens by monoclonal antibodies. Immunology. 1985;56:543-8. (Block)
4. Jang Y, Collector MI, Baylin SB, Diehl AM, Sharkis SJ. Hematopoietic stem cells convert into liver cells within days without fusion. Nat Cell Biol. 2004;6:532-9. (FC)
5. Lee Y, Kim J, Choi K, Choi I, Kim H, Cho S, et al. Enhanced antitumor effect of oncolytic adenovirus expressing interleukin-12 and B7-1 in an immunocompetent murine model. Clin Cancer Res. 2006;12:5859-68. (FC)
6. Choi K, Kim J, Lee Y, Kim J, Suh B, Kim H, et al. Concurrent delivery of GM-CSF and B7-1 using an oncolytic adenovirus elicits potent antitumor effect. Gene Ther. 2006;13:1010-20. (FC)
7. Pevsner-Fischer M, Morad V, Cohen-Sfady M, Rousso-Noori L, Zanin-Zhorov A, Cohen S, et al. Toll-like receptors and their ligands control mesenchymal stem cell functions. Blood. 2007;109:1422-32. (FC)
8. Morad V, Pevsner-Fischer M, Barnees S, Samokovlisky A, Rousso-Noori L, Rosenfeld R, et al. The myelopoietic supportive capacity of mesenchymal stromal cells is uncoupled from multipotency and is influenced by lineage determination and interference with glycosylation. Stem Cells. 2008;26:2275-86. (FC)
9. Avagyan S, Glouchkova L, Choi J, Snoeck H. A quantitative trait locus on chromosome 4 affects cycling of hematopoietic stem and progenitor cells through regulation of TGF-β2 responsiveness. J Immunol. 2008;181:5904-11. (FC)
10. You Y, Zhao H, Wang Y, Carter RH. Cutting edge: Primary and secondary effects of CD19 deficiency on cells of the marginal zone. J Immunol. 2009;182:7343-47. (FC)
11. Shainer R, Azar Y, Almogi-Hazan O, Bringer R, Compton SR, Paidas MJ, et al. Immune regulation and oxidative stress reduction by preimplantation factor following syngeneic or allogenic bone marrow transplantation. Conference Papers in Medicine. 2013;11:1-8. (FC)
12. Azar Y, Shainer R, Almogi-Hazan O, Bringer R, Compton SR, Paidas MJ, et al. Preimplantation factor reduces graft-versus-host disease by regulating immune response and lowering oxidative stress (murine model). Biol Blood Marrow Transplant. 2013;19:519-28. (FC)
13. Knight DA, Ngiow SF, Li M, Parmenter T, Mok S, Cass A, et al. Host immunity contributes to the anti-melanoma activity of BRAF inhibitors. J Clin Invest. 2013;123:1371-81. (FC)
14. Everett J, Turner K, Cai Q, Gordon V, Whiteley M, Rumbaugh K. Arginine is a critical substrate for the pathogenesis of Pseudomonas aeruginosa in burn wound infections. MBio. 2017;8:e02160-16. (IHC-FS)