Rat Anti-Mouse CD18-FITC

Cat. No.
1570-02  
Size
0.5 mg  
Price (USD)
$280.00 
Clone C71/16
Isotype Rat (Lewis) IgG2aκ
Isotype Control Rat IgG2a-FITC
Immunogen Cell membrane glycoproteins derived from mouse BW5147 T cell lymphoma cells
Specificity Mouse CD18
Alternalte Name(s) Integrin β2, ITGB2
Description CD18 represents the common β2 integrin subunit that associates non-covalently with the α/β chains of CD11a, CD11b, and CD11c to form various integrin heterodimers. It is expressed strongly on lymphocytes and monocytes and weakly on granulocytes. CD18 mediates a variety of heterotypic and homotypic intercellular adhesion reactions and it regulates the ligand-binding activates of the various CD11/CD18 complexes. The monoclonal antibody C71/16 has not been reported to inhibit cell adhesion.
Format/Conjugate FITC (Fluorescein)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C; Avoid exposure to light
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 2
Immunohistochemistry-Frozen Sections – Reported in literature 3
Immunohistochemistry-Paraffin Sections – Reported in literature 4
Immunocytochemistry – Reported in literature 2
Immunoprecipitation – Reported in literature 1,2
Western Blot – Reported in literature 2
ELISA – Reported in literature 5
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2794941
Gene ID
16414 (Mouse)
Gene Symbol
Itgb2 (Mouse)
Gene Aliases 2E6; LAD; Cd18; Lfa1; MF17; LCAMB; AI528527
Uniprot ID
P11835 (Mouse)
Uniprot Name
  • BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD18-BIOT (SB Cat. No. 1570-08) followed by Streptavidin-FITC (SB Cat. No. (7100-02).

BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD18-BIOT (SB Cat. No. 1570-08) followed by Streptavidin-FITC (SB Cat. No. (7100-02).
 
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References
1. Trowbridge IS, Omary MB. Molecular complexity of leukocyte surface glycoproteins related to the macrophage differentiation antigen Mac-1. J Exp Med. 1981;154:1517-24. (Immunogen, IP)
2. Choi EY, Orlova VV, Fagerholm SC, Nurmi SM, Chang L, Ballantyne CM, et al. Regulation of LFA-1-dependent inflammatory cell recruitment by Cbl-b and 14-3-3 proteins. Blood. 2008;111:3607-14. (IP, WB, ICC, FC)
3. Bullard DC, Scharffetter-Kochanek K, McArthur MJ, Chosay JG, McBride ME, Montgomery CA, et al. A polygenic mouse model of psoriasiform skin disease in CD18-deficient mice. Proc Natl Acad Sci USA. 1996;93:2116-21. (IHC-FS)
4. Gerth AJ, Lin L, Neurath MF, Glimcher LH, Peng SL. An innate cell-mediated, murine ulcerative colitis-like syndrome in the absence of nuclear factor of activated T cells. Gastroenterology. 2004;126:1115-21. (IHC-PS)
5. Shirakabe K, Shibagaki Y, Yoshimura A, Koyasu S, Hattori S. A proteomic approach for the elucidation of the specificity of ectodomain shedding. J Proteomics. 2014;98:233-43. (ELISA)