Rat Anti-Mouse CD49e-Alexa Fluor® 488

Cat. No.
1695-30  
Size
0.1 mg  
Price (USD)
$190.00 
Clone 5H10.27
Isotype Rat (Lewis) IgG2aκ
Isotype Control Rat IgG2a-Alexa Fluor® 488
Immunogen C57BL/6 x A/J F1 mouse mast cell line MC/9
Specificity Mouse CD49e
Alternalte Name(s) Integrin α5, VLA-5α
Description The monoclonal antibody 5H10.27 reacts with the α5 chain (VLA-5) of the CD49e/CD29 heterodimeric fibronectin receptor. It is expressed on thymocytes, activated T cells, mast cells, and a variety of mouse cell lines. Soluble 5H10.27 antibody inhibits VLA-5-mediated functions in vitro. Immobilized 5H10.27 has also been demonstrated to costimulate the proliferative response of CD8+ T cells to plate-bound anti-CD3 monoclonal antibody.
Format/Conjugate AF488 (Alexa Fluor® 488)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 0.2 mL
Storage & Handling 2-8°C; Avoid exposure to light
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 1,2
Immunohistochemistry-Frozen Sections – Reported in literature 3
Blocking – Reported in literature 1,2
Costimulation – Reported in literature 2
Adhesion – Reported in literature 1,2
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795149
Gene ID
16402 (Mouse)
Gene Symbol
Itga5 (Mouse)
Gene Aliases Fnra; VLA5; Cd49e
Uniprot ID
P11688 (Mouse)
Uniprot Name
  • C57BL/6 mouse bone marrow cells were stained with Rat Anti-Mouse CD49e-AF488 (SB Cat. No. 1695-30).

C57BL/6 mouse bone marrow cells were stained with Rat Anti-Mouse CD49e-AF488 (SB Cat. No. 1695-30).
 
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References
1. Kinashi T, Springer TA. Adhesion molecules in hematopoietic cells. Blood Cells. 1994;20:25-44. (Immunogen, FC, Block, Adhesion)
2. Rich S, Van Nood N, Lee H. Role of α5β1 integrin in TGF-β1-costimulated CD8+ T cell growth and apoptosis. J Immunol. 1996;157:2916-23. (Costim, Block, Adhesion, FC)
3. Schmieder AH, Caruthers SD, Zhang H, Williams TA, Robertson JD, Wickline SA, et al. Three-dimensional MR mapping of angiogenesis with α5β1Vβ3)-targeted theranostic nanoparticles in the MDA-MB-435 xenograft mouse model. FASEB J. 2008;22:4179-89. (IHC-FS)
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