Ensuring timely antibody delivery in the face of supply chain challenges - contact us for solutions

Rat Anti-Mouse CD49e-PE (5H10.27)

Cat. No.:
PE Anti-Mouse CD49e antibody for use in flow cytometry assays.
Size Price (USD) Quantity
0.1 mg $231.00
More Information
Clone 5H10.27
Isotype Rat (Lewis) IgG2aκ
Isotype Control Rat IgG2a-PE (KLH/G2a-1-1)
Specificity Mouse CD49e
Alternative Names Integrin α5, VLA-5α
Description The monoclonal antibody 5H10.27 reacts with the α5 chain (VLA-5) of the CD49e/CD29 heterodimeric fibronectin receptor. It is expressed on thymocytes, activated T cells, mast cells, and a variety of mouse cell lines. Soluble 5H10.27 antibody inhibits VLA-5-mediated functions in vitro. Immobilized 5H10.27 has also been demonstrated to costimulate the proliferative response of CD8+ T cells to plate-bound anti-CD3 monoclonal antibody.
Immunogen C57BL/6 x A/J F1 mouse mast cell line MC/9
Conjugate PE (R-phycoerythrin)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide and a stabilizer
Clonality Monoclonal
Concentration 0.1 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C; Avoid exposure to light; Do not freeze
Applications Flow Cytometry – Quality tested 1,2
Immunohistochemistry-Frozen Sections – Reported in literature 3
Blocking – Reported in literature 1,2
Costimulation – Reported in literature 2
Adhesion – Reported in literature 1,2

RRID Number AB_2795147
Gene ID 16402 (Mouse)
Gene ID Symbol Itga5 (Mouse)
Gene ID Aliases Fnra; VLA5; Cd49e
UniProt ID P11688 (Mouse
UniProt Name ITA5_MOUSE (Mouse)

Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view

Need help with this product?
or call 800.722.2255
  1. 1. Kinashi T, Springer TA. Adhesion molecules in hematopoietic cells. Blood Cells. 1994;20:25-44. (Immunogen, FC, Block, Adhesion)
  2. 2. Rich S, Van Nood N, Lee H. Role of α5β1 integrin in TGF-β1-costimulated CD8+ T cell growth and apoptosis. J Immunol. 1996;157:2916-23. (Costim, Block, Adhesion, FC)
  3. 3. Schmieder AH, Caruthers SD, Zhang H, Williams TA, Robertson JD, Wickline SA, et al. Three-dimensional MR mapping of angiogenesis with α5β1Vβ3)-targeted theranostic nanoparticles in the MDA-MB-435 xenograft mouse model. FASEB J. 2008;22:4179-89. (IHC-FS)