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Rat Anti-Mouse CD49e-AF647 (5H10.27)

Cat. No.:
1695-31
Alexa Fluor® 647 Anti-Mouse CD49e antibody for use in flow cytometry and immunohistochemistry assays.
$219.00
Size Price (USD) Quantity
0.1 mg $219.00
More Information
Clone 5H10.27
Isotype Rat (Lewis) IgG2aκ
Isotype Control Rat IgG2a-AF647 (KLH/G2a-1-1)
Specificity Mouse CD49e
Alternative Names Integrin α5, VLA-5α
Description The monoclonal antibody 5H10.27 reacts with the α5 chain (VLA-5) of the CD49e/CD29 heterodimeric fibronectin receptor. It is expressed on thymocytes, activated T cells, mast cells, and a variety of mouse cell lines. Soluble 5H10.27 antibody inhibits VLA-5-mediated functions in vitro. Immobilized 5H10.27 has also been demonstrated to costimulate the proliferative response of CD8+ T cells to plate-bound anti-CD3 monoclonal antibody.
Immunogen C57BL/6 x A/J F1 mouse mast cell line MC/9
Conjugate AF647 (Alexa Fluor® 647)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Monoclonal
Concentration 0.5 mg/mL
Volume 0.2 mL
Recommended Storage 2-8°C; Avoid exposure to light
Trademark Information Alexa Fluor® is a registered trademark of Thermo Fisher Scientific, Inc. or its subsidiaries
Applications Flow Cytometry – Quality tested 1,2
Immunohistochemistry-Frozen Sections – Reported in literature 3
Blocking – Reported in literature 1,2
Costimulation – Reported in literature 2
Adhesion – Reported in literature 1,2

RRID Number AB_2795150
Gene ID 16402 (Mouse)
Gene ID Symbol Itga5 (Mouse)
Gene ID Aliases Fnra; VLA5; Cd49e
UniProt ID P11688 (Mouse
UniProt Name ITA5_MOUSE (Mouse)

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  1. 1. Kinashi T, Springer TA. Adhesion molecules in hematopoietic cells. Blood Cells. 1994;20:25-44. (Immunogen, FC, Block, Adhesion)
  2. 2. Rich S, Van Nood N, Lee H. Role of α5β1 integrin in TGF-β1-costimulated CD8+ T cell growth and apoptosis. J Immunol. 1996;157:2916-23. (Costim, Block, Adhesion, FC)
  3. 3. Schmieder AH, Caruthers SD, Zhang H, Williams TA, Robertson JD, Wickline SA, et al. Three-dimensional MR mapping of angiogenesis with α5β1(αVβ3)-targeted theranostic nanoparticles in the MDA-MB-435 xenograft mouse model. FASEB J. 2008;22:4179-89. (IHC-FS)