Mouse Anti-Mouse CD72.1-FITC

Cat. No.
1725-02  
Size
0.5 mg  
Price (USD)
$330.00 
Clone 10.1.D2
Isotype Mouse (C57BL/6) IgG2bκ
Isotype Control Mouse IgG2b-FITC
Immunogen DBA/2 mouse spleen cells
Specificity Mouse CD72.1
Alternalte Name(s) Lyb-2.1, CD72a, Lyb-2a
Description Murine CD72.1 (Lyb-2.1), a type II integral membrane glycoprotein and a member of the C-lectin family of cell surface receptors, is a differentiation antigen of B cells and is found in mouse strains expressing the Lyb-2.1 allotype. CD72.1 is the ligand of CD5 which is distributed on all T cells and a small number of B cells. The 10.1.D2 monoclonal antibody blocks binding of CD5 to CD72 on the B cell surface which leads to inhibition of the positive signal resulting from CD5/CD72 pairing. However, 10.1.D2 can itself trigger a positive signal by binding CD72.
Format/Conjugate FITC (Fluorescein)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C; Avoid exposure to light
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 1,4-6
Immunoprecipitation – Reported in literature 2
Activation – Reported in literature 1
Blocking – Reported in literature 5
Costimulation – Reported in literature 3
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795196
Gene ID
12517 (Mouse)
Gene Symbol
Cd72 (Mouse)
Gene Aliases CD72c; Ly-19; Ly-32; Lyb-2; Ly-m19
Uniprot ID
P21855 (Mouse)
Uniprot Name
  • DBA/2 mouse splenocytes were stained with Mouse Anti-Mouse CD72.1-FITC (SB Cat. No. 1725-02) and Rat Anti-Mouse CD19-PE (SB Cat. No. 1575-09).

DBA/2 mouse splenocytes were stained with Mouse Anti-Mouse CD72.1-FITC (SB Cat. No. 1725-02) and Rat Anti-Mouse CD19-PE (SB Cat. No. 1575-09).
 
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References
1. Subbarao B, Mosier DE. Induction of B lymphocyte proliferation by monoclonal anti-Lyb 2 antibody. J Immunol. 1983;130:2033-7. (Immunogen, FC, Activ)
2. Robinson WH, Landolfi MM, Schäfer H, Parnes JR. Biochemical identity of the mouse Ly-19.2 and Ly-32.2 alloantigens with the B cell differentiation antigen Lyb-2/CD72. J Immunol. 1993;151:4764-72. (IP)
3. Snow EC, Mond JJ, Subbarao B. Enhancement by monoclonal anti-Lyb-2 antibody of antigen-specific B lymphocyte expansion stimulated by TNP-Ficoll and T lymphocyte-derived factors. J Immunol. 1986;137:1793-6. (Costim)
4. Robinson WH, Ying H, Miceli MC, Parnes JR. Extensive polymorphism in the extracellular domain of the mouse B cell differentiation antigen Lyb-2/CD72. J Immunol. 1992;149:880-6. (FC)
5. Luo W, van de Velde H, von Hoegen I, Parnes JR, Thielemans K. Ly-1 (CD5), a membrane glycoprotein of mouse T lymphocytes and a subset of B cells, is a natural ligand of the B cell surface protein Lyb-2 (CD72). J Immunol. 1992;148:1630-4. (Block, FC)
6. Zhu Z, Li R, Li H, Zhou T, Davis RS. FCRL5 exerts binary and compartment-specific influence on innate-like B-cell receptor signaling. Proc Natl Acad Sci USA. 2013;110:E1282-90. (FC)