Mouse Anti-Mouse CD72.1-PE
Cat. No.
1725-11
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Size
0.1 mg
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Price (USD)
$245.00
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Clone |
10.1.D2
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Isotype |
Mouse (C57BL/6) IgG2bκ
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Isotype Control |
Mouse IgG2b-APC
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Immunogen |
DBA/2 mouse spleen cells
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Specificity |
Mouse CD72.1
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Alternalte Name(s) |
Lyb-2.1, CD72a, Lyb-2a
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Description |
Murine CD72.1 (Lyb-2.1), a type II integral membrane glycoprotein and a member of the C-lectin family of cell surface receptors, is a differentiation antigen of B cells and is found in mouse strains expressing the Lyb-2.1 allotype. CD72.1 is the ligand of CD5 which is distributed on all T cells and a small number of B cells. The 10.1.D2 monoclonal antibody blocks binding of CD5 to CD72 on the B cell surface which leads to inhibition of the positive signal resulting from CD5/CD72 pairing. However, 10.1.D2 can itself trigger a positive signal by binding CD72.
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Format/Conjugate |
APC (Allophycocyanin)
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Buffer Formulation |
Phosphate buffered saline containing 0.1% sodium azide and a stabilizer
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Concentration |
0.1 mg/mL
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Volume |
1.0 mL
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Storage & Handling |
2-8°C; Avoid exposure to light; Do not freeze
Please refer to product specific SDS
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Applications |
Applications for relevant formats of this clone include - Flow Cytometry – Quality tested 1,4-6 Immunoprecipitation – Reported in literature 2 Activation – Reported in literature 1 Blocking – Reported in literature 5 Costimulation – Reported in literature 3
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Recommended Dilutions |
Please refer to product specific Technical Bulletin
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RRID |
AB_2795199
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Gene ID |
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Gene Symbol |
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Gene Aliases |
CD72c; Ly-19; Ly-32; Lyb-2; Ly-m19
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Uniprot ID |
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Uniprot Name |
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-
DBA/2 mouse splenocytes were stained with Mouse Anti-Mouse CD72.1-APC (SB Cat. No. 1725-11) and Rat Anti-Mouse CD19-FITC (SB Cat. No. 1575-02).
DBA/2 mouse splenocytes were stained with Mouse Anti-Mouse CD72.1-APC (SB Cat. No. 1725-11) and Rat Anti-Mouse CD19-FITC (SB Cat. No. 1575-02).
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References
1. | Subbarao B, Mosier DE. Induction of B lymphocyte proliferation by monoclonal anti-Lyb 2 antibody. J Immunol. 1983;130:2033-7. (Immunogen, FC, Activ) | 2. | Robinson WH, Landolfi MM, Schäfer H, Parnes JR. Biochemical identity of the mouse Ly-19.2 and Ly-32.2 alloantigens with the B cell differentiation antigen Lyb-2/CD72. J Immunol. 1993;151:4764-72. (IP) | 3. | Snow EC, Mond JJ, Subbarao B. Enhancement by monoclonal anti-Lyb-2 antibody of antigen-specific B lymphocyte expansion stimulated by TNP-Ficoll and T lymphocyte-derived factors. J Immunol. 1986;137:1793-6. (Costim) | 4. | Robinson WH, Ying H, Miceli MC, Parnes JR. Extensive polymorphism in the extracellular domain of the mouse B cell differentiation antigen Lyb-2/CD72. J Immunol. 1992;149:880-6. (FC) | 5. | Luo W, van de Velde H, von Hoegen I, Parnes JR, Thielemans K. Ly-1 (CD5), a membrane glycoprotein of mouse T lymphocytes and a subset of B cells, is a natural ligand of the B cell surface protein Lyb-2 (CD72). J Immunol. 1992;148:1630-4. (Block, FC) | 6. | Zhu Z, Li R, Li H, Zhou T, Davis RS. FCRL5 exerts binary and compartment-specific influence on innate-like B-cell receptor signaling. Proc Natl Acad Sci USA. 2013;110:E1282-90. (FC) |
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