Rat Anti-Mouse CD90-SPRD

Cat. No.
1740-13  
Size
0.1 mg  
Price (USD)
$270.00 
Clone G7
Isotype Rat (Lewis) IgG2cκ
Immunogen Mouse T cell hybridoma C6/G8
Specificity Mouse CD90 (Thy-1.1 and Thy1.2 alloantigens/CD90.1 and CD90.2)
Alternalte Name(s) Thy-1
Description CD90 (Thy-1), a GPI-anchored molecule and one of the smallest members of the immunoglobulin superfamily of cell surface receptors, consists of a single V-set domain. It is expressed on thymocytes, peripheral T lymphocytes, some intraepithelial T lymphocytes, and neurons of all mouse strains. The monoclonal antibody G7 stimulates T-cell proliferation and IL-2 secretion via signaling through the T-cell receptor/CD3 complex. G7 has also been reported to promote apoptosis of thymocytes and CTL clones and to mediate adhesion of thymocytes to thymic stroma.
Format/Conjugate SPRD (R-phycoerythrin/Cyanine 5)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide and a stabilizer
Concentration 0.1 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C; Avoid exposure to light; Do not freeze
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 1,6-8
Immunohistochemistry-Paraffin Sections – Reported in literature 4
Immunoprecipitation – Reported in literature 1
Stimulation – Reported in literature 1
Activation – Reported in literature 1,5
Apoptosis – Reported in literature 2,3
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795228
Gene ID
21838 (Mouse)
Gene Symbol
Thy1 (Mouse)
Gene Aliases T25; CD90; Thy-1; Thy1.1; Thy1.2; Thy-1.2
Uniprot ID
P01831 (Mouse)
Uniprot Name
  • BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD90-SPRD (SB Cat. No. 1740-13).
  • C57BL/6 mouse splenocytes were stained with Rat Anti-Mouse CD90-SPRD (SB Cat. No. 1740-13) and Rat Anti-Mouse CD3ε-PE (SB Cat. No. 1535-09).

BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD90-SPRD (SB Cat. No. 1740-13).
 

C57BL/6 mouse splenocytes were stained with Rat Anti-Mouse CD90-SPRD (SB Cat. No. 1740-13) and Rat Anti-Mouse CD3ε-PE (SB Cat. No. 1535-09).
 
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References
1. Gunter KC, Malek TR, Shevach EM. T cell-activating properties of an anti-Thy-1 monoclonal antibody. Possible analogy to OKT3/Leu-4. J Exp Med. 1984;159:716-30. (Immunogen, FC, IP, Stim, Activ)
2. Ucker DS, Meyers J, Obermiller PS. Activation-driven T cell death. II. Quantitative differences alone distinguish stimuli triggering nontransformed T cell proliferation or death. J Immunol. 1992;149:1583-92. (Apop)
3. Hueber A, Raposo G, Pierres M, He H. Thy-1 triggers mouse thymocyte apoptosis through a bcl-2-resistant mechanism. J Exp Med. 1994;179:785-96. (Apop)
4. Whiteland JL, Nicholls SM, Shimeld C, Easty DL, Williams NA, Hill TJ. Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies. J Histochem Cytochem. 1995;43:313-20. (IHC-PS)
5. Kojima H, Toda M, Sitkovsky MV. Comparison of Fas- versus perforin-mediated pathways of cytotoxicity in TCR- and Thy-1-activated murine T cells. Int Immunol. 2000;12:365-74. (Activ)
6. Kucic T, Copland IB, Cuerquis J, Coutu DL, Chalifour LE, Gagnon RF, et al. Mesenchymal stromal cells genetically engineered to overexpress IGF-I enhance cell-based gene therapy of renal failure-induced anemia. Am J Physiol Renal Physiol. 2008;295:F488-96. (FC)
7. Eliopoulos N, Stagg J, Lejeune L, Pommey S, Galipeau J. Allogeneic marrow stromal cells are immune rejected by MHC class I- and class II-mismatched recipient mice. Blood. 2005;106:4057-65. (FC)
8. Xiao J, Angsana J, Wen J, Smith SV, Park PW, Ford ML, et al. Syndecan-1 displays a protective role in aortic aneurysm formation by modulating T cell-mediated responses. Arterioscler Thromb Vasc Biol. 2012;32:386-96. (FC)
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