Rat Anti-Mouse CD90.2-Alexa Fluor® 647

Cat. No.
1750-31  
Size
0.1 mg  
Price (USD)
$190.00 
Clone 30-H12
Isotype Rat (LOU/Ws1/M) IgG2bκ
Isotype Control Rat IgG2b-Alexa Fluor® 647
Immunogen Mouse thymus or spleen
Specificity Mouse CD90.2
Alternalte Name(s) Thy-1.2
Description CD90 (Thy-1) is a GPI-anchored molecule and one of the smallest members of the immunoglobulin superfamily consisting of a single V-set domain. The Thy-1.2 alloantigen is expressed on all thymocytes, peripheral T lymphocytes, and some intraepithelial T cells of most mouse strains. It is also expressed in the brain and at varying levels on other non-lymphoid tissues. The 30-H12 monoclonal antibody does not cross react with mouse and rat strains bearing the Thy-1.1 alloantigen (e.g., AKR/J, PL).
Format/Conjugate AF647 (Alexa Fluor® 647)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 0.2 mL
Storage & Handling 2-8°C; Avoid exposure to light
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 1,2,9,10
Immunohistochemistry-Frozen Sections – Reported in literature 2,3
Immunocytochemistry – Reported in literature 4
Immunoprecipitation – Reported in literature 1
Depletion – Reported in literature 5,6
Costimulation – Reported in literature 7,8
Apoptosis – Reported in literature 7,8
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795242
Gene ID
21838 (Mouse)
Gene Symbol
Thy1 (Mouse)
Gene Aliases T25; CD90; Thy-1; Thy1.1; Thy1.2; Thy-1.2
Uniprot ID
P01831 (Mouse)
Uniprot Name
  • BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD90.2-AF647 (SB Cat. No. 1750-31) and Rat Anti-Mouse CD19-FITC (SB Cat. No. 1575-02).

BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD90.2-AF647 (SB Cat. No. 1750-31) and Rat Anti-Mouse CD19-FITC (SB Cat. No. 1575-02).
 
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References
1. Ledbetter JA, Herzenberg LA. Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens. Immunol Rev. 1979;47:63-90. (Immunogen, FC, IP)
2. Ledbetter JA, Rouse RV, Micklem HS, Herzenberg LA. T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views. J Exp Med. 1980;152:280-95. (FC, IHC-FS)
3. Folgosa L, Zellner HB, El Shikh ME, Conrad DH. Disturbed follicular architecture in B cell A disintegrin and metalloproteinase (ADAM)10 knockouts is mediated by compensatory increases in ADAM17 and TNF-α shedding. J Immunol. 2013;191:5951-8. (IHC-FS)
4. Sharma N, Suzuki H, He Q, Sharma RP. Tumor necrosis factor α-mediated activation of c-Jun NH2-terminal kinase as a mechanism for fumonisin B1 induced apoptosis in murine primary hepatocytes. J Biochem Mol Toxicol. 2005;19:359-67. (ICC)
5. Sharma N, He Q, Sharma RP. Amelioration of fumonisin B1 hepatotoxicity in mice by depletion of T cells with anti-Thy-1.2. Toxicology. 2006;223:191-201. (Depletion)
6. Stevenson MM, Tam MF. Differential induction of helper T cell subsets during blood-stage Plasmodium chabaudi AS infection in resistant and susceptible mice. Clin Exp Immunol. 1993;92:77-83. (Depletion)
7. Nakashima I, Zhang Y, Rahman SM, Yoshida T, Isobe K, Ding L, et al. Evidence of synergy between Thy-1 and CD3/TCR complex in signal delivery to murine thymocytes for cell death. J Immunol. 1991;147:1153-62. (Costim, Apop)
8. Nakashima I, Pu M, Hamaguchi M, Iwamoto T, Rahman SM, Zhang Y, et al. Pathway of signal delivery to murine thymocytes triggered by co-crosslinking CD3 and Thy-1 for cellular DNA fragmentation and growth inhibition. J Immunol. 1993;151:3511-20. (Costim, Apop)
9. Morecki S, Yacovlev E, Gelfand Y, Eizik O, Slavin S. Pretransplant treatment of donors with immunomodulators to control graft-versus-host disease (GVHD) in transplant recipients. Exp Hematol. 2007;35:748-56. (FC)
10. Chaimowitz NS, Martin RK, Cichy J, Gibb DR, Patil P, Kang D, et al. A disintegrin and metalloproteinase 10 regulates antibody production and maintenance of lymphoid architecture. J Immunol. 2011;187:5114-22. (FC)
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