Mouse Anti-Mouse Ly-49F-LE/AF
Cat. No.
1803-14
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Size
0.5 mg
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Price (USD)
$305.00
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Clone |
HBF-719
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Isotype |
Mouse (BALB/c) IgG1κ
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Isotype Control |
Mouse IgG1-LE/AF
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Immunogen |
CHO-K1 cells transfected with B6 allele of Ly49F gene
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Specificity |
Mouse Ly-49F
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Alternalte Name(s) |
Ly-49
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Description |
The monoclonal antibody HBF-719 reacts with the B6 allele of Ly49F, a member of the lectin-like homodimeric Ly49 family of cell surface receptors. Mouse Ly49 receptors exhibit allelic specificity for MHC class I Ia molecules and are thought to serve to prevent natural killer (NK) cells from attacking normal cells while allowing them to attack infected or transformed cells in which class I molecules have been downregulated. These inhibitory receptors are also expressed on subpopulations of mouse CD8+ T cells. The HBF-719 antibody stains ~11% of NK1.1+CD3- splenocytes, and 3.5% (2.5 month old) to 10.0% (11 month old) of CD8+ spleen cells from C57BL/6 mice.
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Format/Conjugate |
LE/AF (Low Endotoxin/Azide Free)
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Buffer Formulation |
Phosphate buffered saline, pH 7.4
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Concentration |
0.5 mg/mL
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Volume |
1.0 mL
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Storage & Handling |
-20°C
Please refer to product specific SDS
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Applications |
Applications for relevant formats of this clone include - Flow Cytometry – Quality tested 1,2
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Recommended Dilutions |
Please refer to product specific Technical Bulletin
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RRID |
AB_2795339
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Gene ID |
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Gene Symbol |
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Gene Aliases |
Ly49f; ly-49f
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Uniprot ID |
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Uniprot Name |
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-
C57BL/6 mouse splenocytes were stained with Mouse Anti-Mouse Ly-49F-PE (SB Cat. No. 1803-09) and Mouse Anti-Mouse NK1.1-FITC (SB Cat. No. 1805-02).
C57BL/6 mouse splenocytes were stained with Mouse Anti-Mouse Ly-49F-PE (SB Cat. No. 1803-09) and Mouse Anti-Mouse NK1.1-FITC (SB Cat. No. 1805-02).
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References
1. | Coles MC, McMahon CW, Takizawa H, Raulet DH. Memory CD8 T lymphocytes express inhibitory MHC-specific Ly49 receptors. Eur J Immunol. 2000;30:236-44. (Immunogen, FC) | 2. | Denning TL, Granger S, Mucida D, Graddy R, Leclercq G, Zhang W, et al. Mouse TCRαβ+CD8αα intraepithelial lymphocytes express genes that down-regulate their antigen reactivity and suppress immune responses. J Immunol. 2007;178:4230-9. (FC) |
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