Mouse Anti-Mouse I-Ek-BIOT
Cat. No.
1898-08
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Size
0.5 mg
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Price (USD)
$225.00
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Clone |
17-3-3
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Isotype |
Mouse (C3H.SW) IgG2aκ
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Isotype Control |
Mouse IgG2a-BIOT
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Immunogen |
C3H mouse skin graft and splenocytes
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Specificity |
Mouse I-Ek
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Alternalte Name(s) |
MHC Class II
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Description |
The monoclonal antibody 17-3-3 reacts with the I-Ek class II alloantigen on cells from mice of the H-2r haplotype. Reactivity with H-2d and H-2p haplotypes has not been observed. Cells from mice of the H-2b, H-2f, H-2g, H-2q, and H-2s haplotypes do not express I-E antigen.
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Format/Conjugate |
BIOT (Biotin)
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Buffer Formulation |
Phosphate buffered saline containing 0.1% sodium azide
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Concentration |
0.5 mg/mL
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Volume |
1.0 mL
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Storage & Handling |
2-8°C
Please refer to product specific SDS
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Applications |
Applications for relevant formats of this clone include - Flow Cytometry – Quality tested 3 Immunohistochemistry-Frozen Sections – Reported in literature 2 Complement Mediated Cell Depletion – Reported in literature 1
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Recommended Dilutions |
Please refer to product specific Technical Bulletin
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RRID |
AB_2795460
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Gene ID |
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Gene Symbol |
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Gene Aliases |
Eb; Ia4; H2Eb; Ia-4; H-2Eb
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Uniprot ID |
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Uniprot Name |
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-
AKR mouse splenocytes were stained with Mouse Anti-Mouse I-Ek-FITC (SB Cat. No. 1898-02) and Rat Anti-Mouse CD3ε-PE (SB Cat. No. 1535-09).
AKR mouse splenocytes were stained with Mouse Anti-Mouse I-Ek-FITC (SB Cat. No. 1898-02) and Rat Anti-Mouse CD3ε-PE (SB Cat. No. 1535-09).
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References
1. | Ozato K, Mayer N, Sachs DH. Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and Ia antigens. J Immunol. 1980;124:533-40. (Immunogen, CMDC) | 2. | Colson YL, Lange J, Fowler K, Ildstad ST. Mechanism for cotolerance in nonlethally conditioned mixed chimeras: negative selection of the Vbeta T-cell receptor repertoire by both host and donor bone marrow-derived cells. Blood. 1996;88:4601-10. (IHC-FS) | 3. | Anderson GD, David CS. In vivo expression and function of hybrid Ia dimers (EαAβ) in recombinant and transgenic mice. J Exp Med. 1989;170:1003-8. (FC) |
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