Rat Anti-Mouse CD107b-UNLB

Cat. No.
1921-01  
Size
0.5 mg  
Price (USD)
$218.00 
Clone ABL-93
Isotype Rat (Sprague Dawley) IgG2aκ
Isotype Control Rat IgG2a-UNLB
Immunogen Glycoproteins purified from BALB/c mouse embryo 3T3 cell line
Specificity Mouse CD107b
Alternalte Name(s) LAMP-2, lysosomal-associated membrane protein 2
Description CD107b, also known as lysosomal-associated membrane protein 2 (LAMP-2), is a heavily glycosylated type I transmembrane protein that constitutes one of the two major sialoglycoproteins on lysosomal membranes that can be used to distinguish lysosomes from other organelles. CD107b is similar in structure to Mac-1 and is expressed primarily on epithelial cells and macrophages in a variety of organs in normal and beige mutant mice.
Format/Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 7
Immunohistochemistry-Frozen Sections – Reported in literature 4
Immunocytochemistry – Reported in literature 1,2,5,6
Electron Microscopy – Reported in literature 1
Immunoprecipitation – Reported in literature 1,2
Western Blot – Reported in literature 2,3
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795535
Gene ID
16784 (Mouse)
Gene Symbol
Lamp2 (Mouse)
Gene Aliases CD107b; LGP-B; Lamp II; Lamp-2; Lamp-2a; Lamp-2b; Lamp-2c; Mac3
Uniprot ID
P17047 (Mouse)
Uniprot Name
  • Mouse Mφ cells were stained with Rat Anti-Mouse CD107b-UNLB (SB Cat. No. 1921-01) followed by Mouse Anti-Rat IgG2a-PE (SB Cat. No. 3065-09).
  • Osteoclasts from ashen mice were stained with AF594 conjugated phalloidin and Rat Anti-Mouse CD107b-UNLB (SB Cat. No. 1921-01) followed by a secondary antibody and DAPI.

    Images from Shimada-Sugawara M, Sakai E, Okamoto K, Fukuda M, Izumi T, Yoshida N, et al. Rab27A regulates transport of cell surface receptors modulating multinucleation and lysosome-related organelles in osteoclasts. Sci Rep. 2015;5:9620. Figure 6(a)

Mouse Mφ cells were stained with Rat Anti-Mouse CD107b-UNLB (SB Cat. No. 1921-01) followed by Mouse Anti-Rat IgG2a-PE (SB Cat. No. 3065-09).
 

Osteoclasts from ashen mice were stained with AF594 conjugated phalloidin and Rat Anti-Mouse CD107b-UNLB (SB Cat. No. 1921-01) followed by a secondary antibody and DAPI.

Images from Shimada-Sugawara M, Sakai E, Okamoto K, Fukuda M, Izumi T, Yoshida N, et al. Rab27A regulates transport of cell surface receptors modulating multinucleation and lysosome-related organelles in osteoclasts. Sci Rep. 2015;5:9620. Figure 6(a)
 
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References
1. Chen JW, Murphy TL, Willingham MC, Pastan I, August JT. Identification of two lysosomal membrane glycoproteins. J Cell Biol. 1985;101:85-95. (Immunogen, ICC, EM, IP)
2. Andrejewski N, Punnonen E, Guhde G, Tanaka Y, Lüllmann-Rauch R, Hartmann D, et al. Normal lysosomal morphology and function in LAMP-1-deficient mice. J Biol Chem. 1999;274:12692-701. (IP, WB, ICC)
3. Via LE, Deretic D, Ulmer RJ, Hibler NS, Huber LA, Deretic V. Arrest of mycobacterial phagosome maturation is caused by a block in vesicle fusion between stages controlled by rab5 and rab7. J Biol Chem. 1997;272:13326-31. (WB)
4. Zhao W, Dumanis SB, Tamboli IY, Rodriguez GA, LaDu MJ, Moussa CE, et al. Human APOE genotype affects intraneuronal Aβ1-42 accumulation in a lentiviral gene transfer model. Hum Mol Genet. 2014;23:1365-75. (IHC-FS)
5. Martina JA, Moriyama K, Bonifacino JS. BLOC-3, a protein complex containing the Hermansky-Pudlak syndrome gene products HPS1 and HPS4. J Biol Chem. 2003;278:29376-84. (ICC)
6. Valk E, Leung R, Kang H, Kaneko K, Rudd CE, Schneider H. T cell receptor-interacting molecule acts as a chaperone to modulate surface expression of the CTLA-4 coreceptor. Immunity. 2006;25:807-21. (ICC)
7. Ramachandra L, Sramkoski RM, Canaday DH, Boom WH, Harding CV. Flow analysis of MHC molecules and other membrane proteins in isolated phagosomes. J Immunol Methods. 1998;213:53-71. (FC)