Mouse Anti-Rabbit IgG-UNLB

Cat. No.
4090-01  
Size
0.5 mg  
Price (USD)
$130.00 
Clone 2A9
Isotype Mouse (BALB/c) IgG1κ
Isotype Control Mouse IgG1-UNLB
Immunogen Recombinant rabbit IgG Fc fragment
Specificity Rabbit IgG Fc
Minimal reactivity to human, mouse, rat, goat, bovine, horse, donkey, sheep, guinea pig, and chicken serum
Format/Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Concentration 0.5 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
ELISA – Quality tested 1-3
FLISA – Quality tested
Flow Cytometry – Quality tested 4,5
Immunohistochemistry-Paraffin Sections – Reported in literature 6
Western Blot – Reported in literature 7
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795999
  • Lane 1 - Rabbit IgG
    Lane 2 - Rabbit IgG Light Chains
    Lane 3 - Rabbit IgG Heavy Chains
    Rabbit immunoglobulins above were resolved by electrophoresis under reducing conditions, transferred to PVDF membrane, and probed with Mouse Anti-Rabbit IgG-HRP (SB Cat. No. 4090-05). Proteins were visualized using chemiluminescent detection.

Lane 1 - Rabbit IgG
Lane 2 - Rabbit IgG Light Chains
Lane 3 - Rabbit IgG Heavy Chains
Rabbit immunoglobulins above were resolved by electrophoresis under reducing conditions, transferred to PVDF membrane, and probed with Mouse Anti-Rabbit IgG-HRP (SB Cat. No. 4090-05). Proteins were visualized using chemiluminescent detection.
 
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References
1. Zolla-Pazner S, Powell R, Yahyaei S, Williams C, Jiang X, Li W, et al. Rationally designed vaccines targeting the V2 region of HIV-1 gp120 induce a focused, cross-clade-reactive, biologically functional antibody response. J Virol. 2016;90:10993-11006. (ELISA)
2. Yamamoto M, Takahashi T, Serada S, Sugase T, Tanaka K, Miyazaki Y, et al. Overexpression of leucine-rich α2-glycoprotein-1 is a prognostic marker and enhances tumor migration in gastric cancer. Cancer Sci. 2017;108:2052-60. (ELISA)
3. Totrov M, Jiang X, Pan R, Zolla-Pazner S, Kong X, inventors; New York University, Molsoft LLC, Ichan School of Medicine at Mount Sinai, assignees. Immunogenic polypeptides. United States patent application publication US 2018/0071400 A1. 2018 Mar 15. (ELISA)
4. Melcher M, Muhle RA, Henrich PP, Kraemer SM, Avril M, Vigan-Womas I, et al. Identification of a role for the PfEMP1 semi-conserved head structure in protein trafficking to the surface of Plasmodium falciparum infected red blood cells. Cell Microbiol. 2010;12:1446-62. (FC)
5. Starkie DO, Compson JE, Rapecki S, Lightwood DJ. Generation of recombinant monoclonal antibodies from immunised mice and rabbits via flow cytometry and sorting of antigen-ppecific IgG+ memory B cells. PLoS One. 2016;11(3):e0152282. (FC)
6. Galles DP. Importância da relação dos ácidos graxos omega-6-omega-3 na alimentação [thesis]. São Paulo (Brazil): University of São Paulo; 2015. (IHC-PS)
7. Hu C, Gong R, Guo A, Chen H. Protective effect of ligand-binding domain of fibronectin-binding protein on mastitis induced by Staphylococcus aureus in mice. Vaccine. 2010;28:4038-44. (WB)
8. Cederquist KB, Liu B, Grima MR, Dalack PJ, Mahorn JT. Laser-fabricated gold nanoparticles for lateral flow immunoassays. Colloids Surf B Biointerfaces. 2017;149:351-7.