Mouse Anti-Porcine CD21-Alexa Fluor® 647

Cat. No.
4530-31  
Size
0.1 mg  
Price (USD)
$235.00 
Clone BB6-11C9.6
Isotype Mouse (BALB/c) IgG1κ
Isotype Control Mouse IgG1-Alexa Fluor® 647
Specificity Porcine CD21
Description CD21, also known as complement receptor 2, is expressed on all mature circulating B lymphocytes but not on T lymphocytes or natural killer cells. Low density CD21 has also been reported on granulocytes and monocytes. The monoclonal antibody BB6-11C9.6 stains the following percentages of surface immunoglobulin (sIg) positive cells in porcine lymphoid tissues - 92-96% in peripheral blood, 74-98% in mesenteric lymph nodes, 97-100% in ileal Peyer’s patches (ILPP), 93-97% in spleen, and 0% in thymus. The antibody does not stain B cells in cord blood or spleen from pig fetuses (105 days gestation).
Format/Conjugate AF647 (Alexa Fluor® 647)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 0.2 mL
Storage & Handling 2-8°C; Avoid exposure to light
Please refer to product specific SDS
Applications
Applications for relevant formats of this clone include -
Flow Cytometry – Quality tested 4,8-21
Immunohistochemistry-Frozen Sections – Reported in literature 1-3
Immunoprecipitation – Reported in literature 4
Separation – Reported in literature 5-8
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2796047
  • Porcine peripheral blood lymphocytes were stained with Mouse Anti-Porcine CD21-AF647 (SB Cat. No. 4530-31) and Mouse Anti-Porcine CD3ε-FITC (SB Cat. No. 4510-02).

Porcine peripheral blood lymphocytes were stained with Mouse Anti-Porcine CD21-AF647 (SB Cat. No. 4530-31) and Mouse Anti-Porcine CD3ε-FITC (SB Cat. No. 4510-02).
 
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References
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