Rabbit Anti-Human IgG(H+L)-BIOT

Cat. No.
6140-08  
Size
1.0 mg  
Price (USD)
$70.00 
Specificity Reacts with the heavy and light chains of human IgG and the light chains of human IgM and IgA
Source Pooled antisera from rabbits hyperimmunized with human IgG
Cross Adsorption None; may react with immunoglobulins from other species
Purification Affinity chromatography on human IgG covalently linked to agarose
Isotype Rabbit IgG
Isotype Control Rabbit IgG-BIOT
Format/Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Concentration 0.5 mg/mL
Volume 2.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications
Quality tested applications for relevant formats include -
ELISA 1-3
FLISA
Other referenced applications for relevant formats include -
Flow Cytometry 4
Immunocytochemistry 5
Immunoprecipitation 5
Western Blot 6
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2796198
  • ELISA plate was coated with purified human IgG, IgM, and IgA. Immunoglobulins were detected with Rabbit Anti-Human IgG(H+L)-BIOT (SB Cat. No. 6140-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).

ELISA plate was coated with purified human IgG, IgM, and IgA. Immunoglobulins were detected with Rabbit Anti-Human IgG(H+L)-BIOT (SB Cat. No. 6140-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
 
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References
1. Yang W, Lam P, Kitching R, Kahn H, Yee A, Aubin JE, et al. Breast cancer metastasis in a human bone NOD/SCID mouse model. Cancer Biol Ther. 2007;6:1295-300. (ELISA)
2. Ahmad G, Zhang W, Torben W, Ahrorov A, Damian RT, Wolf RF, et al. Preclinical prophylactic efficacy testing of Sm-p80-based vaccine in a nonhuman primate model of Schistosoma mansoni infection and immunoglobulin G and E responses to Sm-p80 in human serum samples from an area where schistosomiasis is endemic. J Infect Dis. 2011;204:1437-49. (ELISA)
3. Luo W, Lin S, Lo K, Lu C, Hung C, Chen C, et al. Adaptive immune responses elicited by baculovirus and impacts on subsequent transgene expression in vivo. J Virol. 2013;87:4965-73. (ELISA)
4. Sackstein R. Directing stem cell trafficking via GPS. Methods Enzymol. 2010;479:93-105. (FC)
5. Knight AM, Lucocq JM, Prescott AR, Ponnambalam S, Watts C. Antigen endocytosis and presentation mediated by human membrane IgG1 in the absence of the Igα/Igβ dimer. EMBO J. 1997;16:3842-50. (ICC, IP)
6. Di Zenzo G, Calabresi V, Grosso F, Caproni M, Ruffelli M, Zambruno G. The intracellular and extracellular domains of BP180 antigen comprise novel epitopes targeted by pemphigoid gestationis autoantibodies. J Invest Dermatol. 2007;127:864-73. (WB)