Donkey Anti-Rabbit IgG(H+L), Mouse/Rat/Human SP ads-Alexa Fluor® 555

Cat. No.
6440-32  
Size
1.0 mg  
Price (USD)
$225.00 
Specificity Reacts with the heavy and light chains of rabbit IgG and the light chains of rabbit IgM
Source Pooled antisera from donkeys hyperimmunized with rabbit IgG
Cross Adsorption Mouse, rat, and human serum proteins (SP); may react with immunoglobulins from other species and the light chains of other rabbit immunoglobulins
Purification Affinity chromatography on rabbit IgG covalently linked to agarose
Isotype Donkey IgG
Format/Conjugate AF555 (Alexa Fluor® 555)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide
Concentration 1.0 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C; Avoid exposure to light
Please refer to product specific SDS
Applications
Quality tested applications for relevant formats include -
ELISA 1
FLISA
Other referenced applications for relevant formats include -
Flow Cytometry 2,3
Immunohistochemistry-Paraffin Sections 4,5
Immunocytochemistry 6
Western Blot 7,8
Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2796370
  • Paraffin embedded human gastric cancer tissue was stained with Rabbit IgG-UNLB isotype control (SB Cat. No. 0111-01; left) and Rabbit Anti-Human IgG(H+L), Mouse ads-UNLB (SB Cat. No. 6145-01; right) followed by Donkey Anti-Rabbit IgG(H+L), Mouse/Rat/Human SP ads-AF555 (SB Cat. No. 6440-32) and DAPI.
  • Human hepatocellular carcinoma cell line Hep G2 was stained with Rabbit IgG-UNLB isotype control (SB Cat. No. 0111-01; left) and Rabbit Anti-Human DR5-UNLB (SB Cat. No. 6600-01; right) followed by Donkey Anti-Rabbit IgG(H+L), Mouse/Rat/Human SP ads-AF555 (SB Cat. No. 6440-32) and DAPI.

Paraffin embedded human gastric cancer tissue was stained with Rabbit IgG-UNLB isotype control (SB Cat. No. 0111-01; left) and Rabbit Anti-Human IgG(H+L), Mouse ads-UNLB (SB Cat. No. 6145-01; right) followed by Donkey Anti-Rabbit IgG(H+L), Mouse/Rat/Human SP ads-AF555 (SB Cat. No. 6440-32) and DAPI.
 

Human hepatocellular carcinoma cell line Hep G2 was stained with Rabbit IgG-UNLB isotype control (SB Cat. No. 0111-01; left) and Rabbit Anti-Human DR5-UNLB (SB Cat. No. 6600-01; right) followed by Donkey Anti-Rabbit IgG(H+L), Mouse/Rat/Human SP ads-AF555 (SB Cat. No. 6440-32) and DAPI.
 
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References
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2. So L, Yea SS, Oak JS, Lu M, Manmadhan A, Ke QH, et al. Selective inhibition of phosphoinositide 3-kinase p110 α preserves lymphocyte function. J Biol Chem. 2013;288:5718-31. (FC)
3. Limon JJ, So L, Jellbauer S, Chiu H, Corado J, Sykes SM, et al. mTOR kinase inhibitors promote antibody class switching via mTORC2 inhibition. Proc Natl Acad Sci USA. 2014;111:E5076-85. (FC)
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7. Rasley A, Marriott I, Halberstadt CR, Bost KL, Anguita J. Substance P augments Borrelia burgdorferi-induced prostaglandin E2 production by murine microglia. J Immunol. 2004;172:5707-13. (WB)
8. Hadler-Olsen E, Kanapathippillai P, Berg E, Svineng G, Winberg J, Uhlin-Hansen L. Gelatin in situ zymography on fixed, paraffin-embedded tissue: zinc and ethanol fixation preserve enzyme activity. J Histochem Cytochem. 2010;58:29-39. (WB)
Alexa Fluor® is a registered trademark of Life Technologies Corporation