ELISA plate was coated with serially diluted Rat IgG2c-UNLB (SB Cat. No. 0119-01). Immunoglobulin was detected with Mouse Anti-Rat IgG1-BIOT (SB Cat. No. 3060-08), Mouse Anti-Rat IgG2a-BIOT (SB Cat. No. 3065-08), Mouse Anti-Rat IgG2b-BIMA (SB Cat. No. 3070-28), Mouse Anti-Rat IgG2c-BIOT (SB Cat. No. 3075-08), and Mouse Anti-Rat IgM-BIOT (SB Cat. No. 3080-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.